Relevance of Oxygen Concentration in Stem Cell Culture for Regenerative Medicine
The key hallmark of stem cells is their ability to self-renew while keeping a differentiation potential. Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. One of these factors is oxygen concentratio...
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| Veröffentlicht in: | International journal of molecular sciences 2019-03, Vol.20 (5), p.1195 |
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| creator | Mas-Bargues, Cristina Sanz-Ros, Jorge Román-Domínguez, Aurora Inglés, Marta Gimeno-Mallench, Lucia El Alami, Marya Viña-Almunia, José Gambini, Juan Viña, José Borrás, Consuelo |
| description | The key hallmark of stem cells is their ability to self-renew while keeping a differentiation potential. Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. One of these factors is oxygen concentration, which has been closely linked to the maintenance of stemness. The widely used environmental 21% O₂ concentration represents a hyperoxic non-physiological condition, which can impair stem cell behaviour by many mechanisms. The goal of this review is to understand these mechanisms underlying the oxygen signalling pathways and their negatively-associated consequences. This may provide a rationale for culturing stem cells under physiological oxygen concentration for stem cell therapy success, in the field of tissue engineering and regenerative medicine. |
| doi_str_mv | 10.3390/ijms20051195 |
| format | Article |
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Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. One of these factors is oxygen concentration, which has been closely linked to the maintenance of stemness. The widely used environmental 21% O₂ concentration represents a hyperoxic non-physiological condition, which can impair stem cell behaviour by many mechanisms. The goal of this review is to understand these mechanisms underlying the oxygen signalling pathways and their negatively-associated consequences. This may provide a rationale for culturing stem cells under physiological oxygen concentration for stem cell therapy success, in the field of tissue engineering and regenerative medicine.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20051195</identifier><identifier>PMID: 30857245</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Bibliographic literature ; Bone marrow ; Cell culture ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Self Renewal ; Cellular Senescence ; Growth factors ; Humans ; Lungs ; Organs ; Oxidation-Reduction ; Oxygen - metabolism ; Physiology ; Regeneration ; Regenerative medicine ; Regenerative Medicine - methods ; Replication ; Retina ; Review ; Signal Transduction ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Tissue engineering ; Tissue Engineering - methods ; Vascularization</subject><ispartof>International journal of molecular sciences, 2019-03, Vol.20 (5), p.1195</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). 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Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. One of these factors is oxygen concentration, which has been closely linked to the maintenance of stemness. The widely used environmental 21% O₂ concentration represents a hyperoxic non-physiological condition, which can impair stem cell behaviour by many mechanisms. The goal of this review is to understand these mechanisms underlying the oxygen signalling pathways and their negatively-associated consequences. 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| subjects | Animals Bibliographic literature Bone marrow Cell culture Cell Culture Techniques - methods Cell Differentiation Cell Self Renewal Cellular Senescence Growth factors Humans Lungs Organs Oxidation-Reduction Oxygen - metabolism Physiology Regeneration Regenerative medicine Regenerative Medicine - methods Replication Retina Review Signal Transduction Stem cells Stem Cells - cytology Stem Cells - metabolism Tissue engineering Tissue Engineering - methods Vascularization |
| title | Relevance of Oxygen Concentration in Stem Cell Culture for Regenerative Medicine |
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