Diagnosis of Parapneumonia Pleural Effusion with Serum and Pleural Fluid Cell-Free DNA
Objective. As cell-free DNA levels in the pleural fluid and serum of parapneumonic pleural effusion (PPE) patients have not been thoroughly explored, we evaluated their diagnostic potential. Methods. Twenty-two PPE and 16 non-PPE patients were evaluated. Serum and pleural fluids were collected, and...
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| Veröffentlicht in: | BioMed research international 2019-01, Vol.2019 (2019), p.1-8 |
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| creator | Su, Yu-Jih Lu, Cheng-Hsien Cheng, Ben-Chung Wang, Hung-Chen Huang, Chih-Cheng Lai, Yun-Ru Tsai, Nai-Wen Hsiao, Sheng-Yuan Kung, Chia-Te Su, Chih-Min Lin, Wei-Che |
| description | Objective. As cell-free DNA levels in the pleural fluid and serum of parapneumonic pleural effusion (PPE) patients have not been thoroughly explored, we evaluated their diagnostic potential. Methods. Twenty-two PPE and 16 non-PPE patients were evaluated. Serum and pleural fluids were collected, and cell-free DNA was quantified. All biomarkers were assessed for correlation with days after admission. Receiver operating characteristic (ROC) curve analysis was used to determine diagnostic accuracy and optimal cut-off point. Results. Nuclear and mitochondrial DNA levels in the pleural fluid and nuclear DNA levels in serum of PPE patients were significantly higher than in those of the non-PPE patients. However, only cell-free DNA levels in pleural fluid correlated with days after admission among PPE patients (r= 0.464, 0.538, respectively). ROC curve analysis showed that nuclear and mitochondrial DNA in pleural fluid had AUCs of 0.945 and 0.889, respectively. With cut-off values of 134.9 and 17.8 ng/ml for nuclear and mitochondrial DNA in pleural fluid, respectively, 96% sensitivity and 81% specificity were observed for PPE diagnosis. Conclusion. Nuclear and mitochondrial DNA in pleural fluid possess PPE diagnostic potential and correlated with disease severity. Serum nuclear DNA could also be used to distinguish freshly admitted PPE patients (Day 1) from non-PPE patients, but with less accuracy. |
| doi_str_mv | 10.1155/2019/5028512 |
| format | Article |
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As cell-free DNA levels in the pleural fluid and serum of parapneumonic pleural effusion (PPE) patients have not been thoroughly explored, we evaluated their diagnostic potential. Methods. Twenty-two PPE and 16 non-PPE patients were evaluated. Serum and pleural fluids were collected, and cell-free DNA was quantified. All biomarkers were assessed for correlation with days after admission. Receiver operating characteristic (ROC) curve analysis was used to determine diagnostic accuracy and optimal cut-off point. Results. Nuclear and mitochondrial DNA levels in the pleural fluid and nuclear DNA levels in serum of PPE patients were significantly higher than in those of the non-PPE patients. However, only cell-free DNA levels in pleural fluid correlated with days after admission among PPE patients (r= 0.464, 0.538, respectively). ROC curve analysis showed that nuclear and mitochondrial DNA in pleural fluid had AUCs of 0.945 and 0.889, respectively. With cut-off values of 134.9 and 17.8 ng/ml for nuclear and mitochondrial DNA in pleural fluid, respectively, 96% sensitivity and 81% specificity were observed for PPE diagnosis. Conclusion. Nuclear and mitochondrial DNA in pleural fluid possess PPE diagnostic potential and correlated with disease severity. Serum nuclear DNA could also be used to distinguish freshly admitted PPE patients (Day 1) from non-PPE patients, but with less accuracy.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2019/5028512</identifier><identifier>PMID: 30949501</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Aged ; Antibiotics ; Bioindicators ; Biomarkers ; Biomedical research ; Blood ; Cell-Free Nucleic Acids - blood ; Dehydrogenases ; Deoxyribonucleic acid ; Diagnosis ; Diagnostic systems ; Disease ; DNA ; DNA, Mitochondrial - blood ; Emergency medical care ; Female ; Fluids ; Hospitals ; Humans ; Laboratories ; Male ; Middle Aged ; Mitochondrial DNA ; Mortality ; Patients ; Pleura - metabolism ; Pleural effusion ; Pleural Effusion - blood ; Pleural Effusion - diagnosis ; Pleural effusions ; Pleural fluid ; Pneumonia ; Pneumonia - blood ; Pneumonia - diagnosis ; Proteins ; Quantitative analysis</subject><ispartof>BioMed research international, 2019-01, Vol.2019 (2019), p.1-8</ispartof><rights>Copyright © 2019 Chih-Min Su et al.</rights><rights>COPYRIGHT 2019 John Wiley & Sons, Inc.</rights><rights>Copyright © 2019 Chih-Min Su et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Chih-Min Su et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-cf9360a9fbc78f89769795f3f411f35d295a2537ea7c85d9de9049bb9a5e79533</citedby><cites>FETCH-LOGICAL-c499t-cf9360a9fbc78f89769795f3f411f35d295a2537ea7c85d9de9049bb9a5e79533</cites><orcidid>0000-0003-2960-824X ; 0000-0003-3962-1958 ; 0000-0002-6881-5121 ; 0000-0002-5258-8569 ; 0000-0002-7274-3458 ; 0000-0002-5716-3930 ; 0000-0002-6743-7579</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425321/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425321/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30949501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tulkens, Paul M.</contributor><contributor>Paul M Tulkens</contributor><creatorcontrib>Su, Yu-Jih</creatorcontrib><creatorcontrib>Lu, Cheng-Hsien</creatorcontrib><creatorcontrib>Cheng, Ben-Chung</creatorcontrib><creatorcontrib>Wang, Hung-Chen</creatorcontrib><creatorcontrib>Huang, Chih-Cheng</creatorcontrib><creatorcontrib>Lai, Yun-Ru</creatorcontrib><creatorcontrib>Tsai, Nai-Wen</creatorcontrib><creatorcontrib>Hsiao, Sheng-Yuan</creatorcontrib><creatorcontrib>Kung, Chia-Te</creatorcontrib><creatorcontrib>Su, Chih-Min</creatorcontrib><creatorcontrib>Lin, Wei-Che</creatorcontrib><title>Diagnosis of Parapneumonia Pleural Effusion with Serum and Pleural Fluid Cell-Free DNA</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Objective. As cell-free DNA levels in the pleural fluid and serum of parapneumonic pleural effusion (PPE) patients have not been thoroughly explored, we evaluated their diagnostic potential. Methods. Twenty-two PPE and 16 non-PPE patients were evaluated. Serum and pleural fluids were collected, and cell-free DNA was quantified. All biomarkers were assessed for correlation with days after admission. Receiver operating characteristic (ROC) curve analysis was used to determine diagnostic accuracy and optimal cut-off point. Results. Nuclear and mitochondrial DNA levels in the pleural fluid and nuclear DNA levels in serum of PPE patients were significantly higher than in those of the non-PPE patients. However, only cell-free DNA levels in pleural fluid correlated with days after admission among PPE patients (r= 0.464, 0.538, respectively). ROC curve analysis showed that nuclear and mitochondrial DNA in pleural fluid had AUCs of 0.945 and 0.889, respectively. With cut-off values of 134.9 and 17.8 ng/ml for nuclear and mitochondrial DNA in pleural fluid, respectively, 96% sensitivity and 81% specificity were observed for PPE diagnosis. Conclusion. Nuclear and mitochondrial DNA in pleural fluid possess PPE diagnostic potential and correlated with disease severity. Serum nuclear DNA could also be used to distinguish freshly admitted PPE patients (Day 1) from non-PPE patients, but with less accuracy.</description><subject>Aged</subject><subject>Antibiotics</subject><subject>Bioindicators</subject><subject>Biomarkers</subject><subject>Biomedical research</subject><subject>Blood</subject><subject>Cell-Free Nucleic Acids - blood</subject><subject>Dehydrogenases</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Disease</subject><subject>DNA</subject><subject>DNA, Mitochondrial - blood</subject><subject>Emergency medical care</subject><subject>Female</subject><subject>Fluids</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mitochondrial DNA</subject><subject>Mortality</subject><subject>Patients</subject><subject>Pleura - metabolism</subject><subject>Pleural effusion</subject><subject>Pleural Effusion - blood</subject><subject>Pleural Effusion - diagnosis</subject><subject>Pleural effusions</subject><subject>Pleural fluid</subject><subject>Pneumonia</subject><subject>Pneumonia - blood</subject><subject>Pneumonia - diagnosis</subject><subject>Proteins</subject><subject>Quantitative analysis</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9rFDEUx4NYbFl78ywBL4KOzc-ZyUVYtt0qFC1YvYbszMtuykyyTTYW__tm2e3WejKXBN6HT957X4TeUPKJUinPGKHqTBLWSspeoBPGqahqKujLw5vzY3Sa0i0pp6U1UfUrdMyJEkoSeoJ-nTuz9CG5hIPF1yaatYc8Bu8Mvh4gRzPgC2tzcsHje7dZ4R8Q84iN7w_1-ZBdj2cwDNU8AuDzb9PX6MiaIcHp_p6gn_OLm9mX6ur75dfZ9KrqhFKbqrOK18Qou-ia1raqqVWjpOVWUGq57JmShknegGm6VvaqB0WEWiyUkVBAzifo8867zosR-g78pnSk19GNJv7RwTj9vOLdSi_Db12L4mW0CN7vBTHcZUgbPbrUlVGMh5CTZoyIWlHSyoK--we9DTn6Mp5mVPGy6Va2T9TSDKCdt6H8222lelozIWgjCjxBH3dUF0NKEeyhZUr0Nlm9TVbvky3427_HPMCPORbgww5YOd-be_efOigMWPNEUyabWvAHyVmyTA</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Su, Yu-Jih</creator><creator>Lu, Cheng-Hsien</creator><creator>Cheng, Ben-Chung</creator><creator>Wang, Hung-Chen</creator><creator>Huang, Chih-Cheng</creator><creator>Lai, Yun-Ru</creator><creator>Tsai, Nai-Wen</creator><creator>Hsiao, Sheng-Yuan</creator><creator>Kung, Chia-Te</creator><creator>Su, Chih-Min</creator><creator>Lin, Wei-Che</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2960-824X</orcidid><orcidid>https://orcid.org/0000-0003-3962-1958</orcidid><orcidid>https://orcid.org/0000-0002-6881-5121</orcidid><orcidid>https://orcid.org/0000-0002-5258-8569</orcidid><orcidid>https://orcid.org/0000-0002-7274-3458</orcidid><orcidid>https://orcid.org/0000-0002-5716-3930</orcidid><orcidid>https://orcid.org/0000-0002-6743-7579</orcidid></search><sort><creationdate>20190101</creationdate><title>Diagnosis of Parapneumonia Pleural Effusion with Serum and Pleural Fluid Cell-Free DNA</title><author>Su, Yu-Jih ; Lu, Cheng-Hsien ; Cheng, Ben-Chung ; Wang, Hung-Chen ; Huang, Chih-Cheng ; Lai, Yun-Ru ; Tsai, Nai-Wen ; Hsiao, Sheng-Yuan ; Kung, Chia-Te ; Su, Chih-Min ; Lin, Wei-Che</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-cf9360a9fbc78f89769795f3f411f35d295a2537ea7c85d9de9049bb9a5e79533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aged</topic><topic>Antibiotics</topic><topic>Bioindicators</topic><topic>Biomarkers</topic><topic>Biomedical research</topic><topic>Blood</topic><topic>Cell-Free Nucleic Acids - blood</topic><topic>Dehydrogenases</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Disease</topic><topic>DNA</topic><topic>DNA, Mitochondrial - blood</topic><topic>Emergency medical care</topic><topic>Female</topic><topic>Fluids</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Laboratories</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mitochondrial DNA</topic><topic>Mortality</topic><topic>Patients</topic><topic>Pleura - metabolism</topic><topic>Pleural effusion</topic><topic>Pleural Effusion - blood</topic><topic>Pleural Effusion - diagnosis</topic><topic>Pleural effusions</topic><topic>Pleural fluid</topic><topic>Pneumonia</topic><topic>Pneumonia - blood</topic><topic>Pneumonia - diagnosis</topic><topic>Proteins</topic><topic>Quantitative analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Yu-Jih</creatorcontrib><creatorcontrib>Lu, Cheng-Hsien</creatorcontrib><creatorcontrib>Cheng, Ben-Chung</creatorcontrib><creatorcontrib>Wang, Hung-Chen</creatorcontrib><creatorcontrib>Huang, Chih-Cheng</creatorcontrib><creatorcontrib>Lai, Yun-Ru</creatorcontrib><creatorcontrib>Tsai, Nai-Wen</creatorcontrib><creatorcontrib>Hsiao, Sheng-Yuan</creatorcontrib><creatorcontrib>Kung, Chia-Te</creatorcontrib><creatorcontrib>Su, Chih-Min</creatorcontrib><creatorcontrib>Lin, Wei-Che</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Yu-Jih</au><au>Lu, Cheng-Hsien</au><au>Cheng, Ben-Chung</au><au>Wang, Hung-Chen</au><au>Huang, Chih-Cheng</au><au>Lai, Yun-Ru</au><au>Tsai, Nai-Wen</au><au>Hsiao, Sheng-Yuan</au><au>Kung, Chia-Te</au><au>Su, Chih-Min</au><au>Lin, Wei-Che</au><au>Tulkens, Paul M.</au><au>Paul M Tulkens</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnosis of Parapneumonia Pleural Effusion with Serum and Pleural Fluid Cell-Free DNA</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Objective. As cell-free DNA levels in the pleural fluid and serum of parapneumonic pleural effusion (PPE) patients have not been thoroughly explored, we evaluated their diagnostic potential. Methods. Twenty-two PPE and 16 non-PPE patients were evaluated. Serum and pleural fluids were collected, and cell-free DNA was quantified. All biomarkers were assessed for correlation with days after admission. Receiver operating characteristic (ROC) curve analysis was used to determine diagnostic accuracy and optimal cut-off point. Results. Nuclear and mitochondrial DNA levels in the pleural fluid and nuclear DNA levels in serum of PPE patients were significantly higher than in those of the non-PPE patients. However, only cell-free DNA levels in pleural fluid correlated with days after admission among PPE patients (r= 0.464, 0.538, respectively). ROC curve analysis showed that nuclear and mitochondrial DNA in pleural fluid had AUCs of 0.945 and 0.889, respectively. With cut-off values of 134.9 and 17.8 ng/ml for nuclear and mitochondrial DNA in pleural fluid, respectively, 96% sensitivity and 81% specificity were observed for PPE diagnosis. Conclusion. Nuclear and mitochondrial DNA in pleural fluid possess PPE diagnostic potential and correlated with disease severity. Serum nuclear DNA could also be used to distinguish freshly admitted PPE patients (Day 1) from non-PPE patients, but with less accuracy.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>30949501</pmid><doi>10.1155/2019/5028512</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2960-824X</orcidid><orcidid>https://orcid.org/0000-0003-3962-1958</orcidid><orcidid>https://orcid.org/0000-0002-6881-5121</orcidid><orcidid>https://orcid.org/0000-0002-5258-8569</orcidid><orcidid>https://orcid.org/0000-0002-7274-3458</orcidid><orcidid>https://orcid.org/0000-0002-5716-3930</orcidid><orcidid>https://orcid.org/0000-0002-6743-7579</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Antibiotics Bioindicators Biomarkers Biomedical research Blood Cell-Free Nucleic Acids - blood Dehydrogenases Deoxyribonucleic acid Diagnosis Diagnostic systems Disease DNA DNA, Mitochondrial - blood Emergency medical care Female Fluids Hospitals Humans Laboratories Male Middle Aged Mitochondrial DNA Mortality Patients Pleura - metabolism Pleural effusion Pleural Effusion - blood Pleural Effusion - diagnosis Pleural effusions Pleural fluid Pneumonia Pneumonia - blood Pneumonia - diagnosis Proteins Quantitative analysis |
| title | Diagnosis of Parapneumonia Pleural Effusion with Serum and Pleural Fluid Cell-Free DNA |
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