Video-rate quantitative phase analysis by a DIC microscope using a polarization camera

Video-rate quantitative phase analysis by a DIC microscope using a polarization camera

This paper describes how to take advantage of the replacement of an intensity camera with a polarization camera in a standard differential interference contrast (DIC) microscope. Using a polarization camera enables snapshot quantitative phase analysis so that real-time imaging of living transparent...

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Veröffentlicht in:Biomedical optics express 2019-03, Vol.10 (3), p.1273-1281
Hauptverfasser: Shibata, Shuhei, Takano, Wataru, Hagen, Nathan, Matsuda, Masaru, Otani, Yukitoshi
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container_title Biomedical optics express
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creator Shibata, Shuhei
Takano, Wataru
Hagen, Nathan
Matsuda, Masaru
Otani, Yukitoshi
description This paper describes how to take advantage of the replacement of an intensity camera with a polarization camera in a standard differential interference contrast (DIC) microscope. Using a polarization camera enables snapshot quantitative phase analysis so that real-time imaging of living transparent tissues become possible. Using our method, we quantify the phase measurement accuracy using a phantom consisting of glass beads embedded in lacquer. In order to demonstrate these advantages, we image the pumping heart and blood flow in a living egg.
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title Video-rate quantitative phase analysis by a DIC microscope using a polarization camera
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