Thermodynamic and structural contributions of the 6-thioguanosine residue to helical properties of RNA
Thionucleotides, especially 4-thiouridine and 6-thioguanosine, are photosensitive molecules that photocrosslink to both proteins and nucleic acids, and this feature is a major reason for their application in various investigations. To get insight into the thermodynamic and structural contributions o...
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| Veröffentlicht in: | Scientific reports 2019-03, Vol.9 (1), p.4385, Article 4385 |
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| description | Thionucleotides, especially 4-thiouridine and 6-thioguanosine, are photosensitive molecules that photocrosslink to both proteins and nucleic acids, and this feature is a major reason for their application in various investigations. To get insight into the thermodynamic and structural contributions of 6-thioguanosine to the properties of RNA duplexes a systematic study was performed. In a series of RNA duplexes, selected guanosine residues located in G-C base pairs, mismatches (G-G, G-U, and G-A), or 5′ and 3′-dangling ends were replaced with 6-thioguanosine. Generally, the presence of 6-thioguanosine diminishes the thermodynamic stability of RNA duplexes. This effect depends on its position within duplexes and the sequence of adjacent base pairs. However, when placed at a dangling end a 6-thioguanosine residue actually exerts a weak stabilizing effect. Furthermore, the structural effect of 6-thioguanosine substitution appears to be minimal based on NMR and Circular Dichroism (CD) data. |
| doi_str_mv | 10.1038/s41598-019-40715-2 |
| format | Article |
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To get insight into the thermodynamic and structural contributions of 6-thioguanosine to the properties of RNA duplexes a systematic study was performed. In a series of RNA duplexes, selected guanosine residues located in G-C base pairs, mismatches (G-G, G-U, and G-A), or 5′ and 3′-dangling ends were replaced with 6-thioguanosine. Generally, the presence of 6-thioguanosine diminishes the thermodynamic stability of RNA duplexes. This effect depends on its position within duplexes and the sequence of adjacent base pairs. However, when placed at a dangling end a 6-thioguanosine residue actually exerts a weak stabilizing effect. Furthermore, the structural effect of 6-thioguanosine substitution appears to be minimal based on NMR and Circular Dichroism (CD) data.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-40715-2</identifier><identifier>PMID: 30867505</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/45/500 ; 631/45/535/878/1263 ; 631/92/2783 ; Adenosine ; Base pairs ; Base Sequence ; Chloride ; Circular Dichroism ; Data processing ; Guanine Nucleotides - chemistry ; Guanosine ; Guanosine - analogs & derivatives ; Guanosine - chemistry ; Humanities and Social Sciences ; Magnetic Resonance Spectroscopy ; multidisciplinary ; Nucleic Acid Conformation ; Nucleic acids ; Nucleotide sequence ; Organophosphorus Compounds - chemistry ; Proteins ; Ribonucleic acid ; RNA ; RNA - chemistry ; RNA, Double-Stranded ; Science ; Science (multidisciplinary) ; Thionucleosides - chemistry</subject><ispartof>Scientific reports, 2019-03, Vol.9 (1), p.4385, Article 4385</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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To get insight into the thermodynamic and structural contributions of 6-thioguanosine to the properties of RNA duplexes a systematic study was performed. In a series of RNA duplexes, selected guanosine residues located in G-C base pairs, mismatches (G-G, G-U, and G-A), or 5′ and 3′-dangling ends were replaced with 6-thioguanosine. Generally, the presence of 6-thioguanosine diminishes the thermodynamic stability of RNA duplexes. This effect depends on its position within duplexes and the sequence of adjacent base pairs. However, when placed at a dangling end a 6-thioguanosine residue actually exerts a weak stabilizing effect. 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To get insight into the thermodynamic and structural contributions of 6-thioguanosine to the properties of RNA duplexes a systematic study was performed. In a series of RNA duplexes, selected guanosine residues located in G-C base pairs, mismatches (G-G, G-U, and G-A), or 5′ and 3′-dangling ends were replaced with 6-thioguanosine. Generally, the presence of 6-thioguanosine diminishes the thermodynamic stability of RNA duplexes. This effect depends on its position within duplexes and the sequence of adjacent base pairs. However, when placed at a dangling end a 6-thioguanosine residue actually exerts a weak stabilizing effect. Furthermore, the structural effect of 6-thioguanosine substitution appears to be minimal based on NMR and Circular Dichroism (CD) data.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30867505</pmid><doi>10.1038/s41598-019-40715-2</doi><orcidid>https://orcid.org/0000-0002-9218-1077</orcidid><orcidid>https://orcid.org/0000-0003-2955-1829</orcidid><orcidid>https://orcid.org/0000-0001-6951-2715</orcidid><orcidid>https://orcid.org/0000-0002-8124-1003</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 631/45/500 631/45/535/878/1263 631/92/2783 Adenosine Base pairs Base Sequence Chloride Circular Dichroism Data processing Guanine Nucleotides - chemistry Guanosine Guanosine - analogs & derivatives Guanosine - chemistry Humanities and Social Sciences Magnetic Resonance Spectroscopy multidisciplinary Nucleic Acid Conformation Nucleic acids Nucleotide sequence Organophosphorus Compounds - chemistry Proteins Ribonucleic acid RNA RNA - chemistry RNA, Double-Stranded Science Science (multidisciplinary) Thionucleosides - chemistry |
| title | Thermodynamic and structural contributions of the 6-thioguanosine residue to helical properties of RNA |
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