Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198
Potato ( L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to...
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| Veröffentlicht in: | International journal of molecular sciences 2019-02, Vol.20 (4), p.852 |
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| creator | Yang, Xiaohui Liu, Jie Xu, Jianfei Duan, Shaoguang Wang, Qianru Li, Guangcun Jin, Liping |
| description | Potato (
L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to drought stress. Here, a time-course experiment was performed to identify drought resistance response genes in P3-198 under polyethylene glycol (PEG)-induced stress using RNA-sequencing. A total of 1665 differentially expressed genes (DEGs) were specifically identified, and based on gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the transcription factor activity, protein kinase activity, and the plant hormone signal transduction process were significantly enriched. Annotation revealed that these DEGs mainly encode transcription factors, protein kinases, and proteins related to redox regulation, carbohydrate metabolism, and osmotic adjustment. In particular, genes encoding abscisic acid (ABA)-dependent signaling molecules were significantly differentially expressed, which revealed the important roles of the ABA-dependent signaling pathway in the early response of P3-198 to drought stress. Quantitative real-time PCR experimental verification confirmed the differential expression of genes in the drought resistance signaling pathway. Our results provide valuable information for understanding potato drought-resistance mechanisms, and also enrich the gene resources available for drought-resistant potato breeding. |
| doi_str_mv | 10.3390/ijms20040852 |
| format | Article |
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L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to drought stress. Here, a time-course experiment was performed to identify drought resistance response genes in P3-198 under polyethylene glycol (PEG)-induced stress using RNA-sequencing. A total of 1665 differentially expressed genes (DEGs) were specifically identified, and based on gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the transcription factor activity, protein kinase activity, and the plant hormone signal transduction process were significantly enriched. Annotation revealed that these DEGs mainly encode transcription factors, protein kinases, and proteins related to redox regulation, carbohydrate metabolism, and osmotic adjustment. In particular, genes encoding abscisic acid (ABA)-dependent signaling molecules were significantly differentially expressed, which revealed the important roles of the ABA-dependent signaling pathway in the early response of P3-198 to drought stress. Quantitative real-time PCR experimental verification confirmed the differential expression of genes in the drought resistance signaling pathway. Our results provide valuable information for understanding potato drought-resistance mechanisms, and also enrich the gene resources available for drought-resistant potato breeding.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20040852</identifier><identifier>PMID: 30781424</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Abscisic acid ; Breeding ; Cultivars ; Dehydration ; Deoxyribonucleic acid ; Diploids ; Diploidy ; DNA ; Drought ; Drought resistance ; Droughts ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Gene Ontology ; Genomes ; Genotype ; Genotypes ; Kinases ; Molecular modelling ; Nucleotide sequence ; Osmosis ; Phenotype ; Phosphotransferase ; Polyethylene Glycols - pharmacology ; Potatoes ; Protein kinase ; Protein Kinases - metabolism ; Proteins ; Reproducibility of Results ; Solanum tuberosum - genetics ; Solanum tuberosum - physiology ; Stress, Physiological - genetics ; Transcription Factors - metabolism</subject><ispartof>International journal of molecular sciences, 2019-02, Vol.20 (4), p.852</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-c0efaa70b4cd3e2a8fedf6ba957e852753d595bfe94f1a52553c9489f84ab5e33</citedby><cites>FETCH-LOGICAL-c412t-c0efaa70b4cd3e2a8fedf6ba957e852753d595bfe94f1a52553c9489f84ab5e33</cites><orcidid>0000-0002-3297-0242 ; 0000-0001-9119-3474 ; 0000-0003-2024-9914</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413097/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6413097/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30781424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiaohui</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Xu, Jianfei</creatorcontrib><creatorcontrib>Duan, Shaoguang</creatorcontrib><creatorcontrib>Wang, Qianru</creatorcontrib><creatorcontrib>Li, Guangcun</creatorcontrib><creatorcontrib>Jin, Liping</creatorcontrib><title>Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Potato (
L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to drought stress. Here, a time-course experiment was performed to identify drought resistance response genes in P3-198 under polyethylene glycol (PEG)-induced stress using RNA-sequencing. A total of 1665 differentially expressed genes (DEGs) were specifically identified, and based on gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the transcription factor activity, protein kinase activity, and the plant hormone signal transduction process were significantly enriched. Annotation revealed that these DEGs mainly encode transcription factors, protein kinases, and proteins related to redox regulation, carbohydrate metabolism, and osmotic adjustment. In particular, genes encoding abscisic acid (ABA)-dependent signaling molecules were significantly differentially expressed, which revealed the important roles of the ABA-dependent signaling pathway in the early response of P3-198 to drought stress. Quantitative real-time PCR experimental verification confirmed the differential expression of genes in the drought resistance signaling pathway. Our results provide valuable information for understanding potato drought-resistance mechanisms, and also enrich the gene resources available for drought-resistant potato breeding.</description><subject>Abscisic acid</subject><subject>Breeding</subject><subject>Cultivars</subject><subject>Dehydration</subject><subject>Deoxyribonucleic acid</subject><subject>Diploids</subject><subject>Diploidy</subject><subject>DNA</subject><subject>Drought</subject><subject>Drought resistance</subject><subject>Droughts</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Ontology</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Kinases</subject><subject>Molecular modelling</subject><subject>Nucleotide sequence</subject><subject>Osmosis</subject><subject>Phenotype</subject><subject>Phosphotransferase</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Potatoes</subject><subject>Protein kinase</subject><subject>Protein Kinases - metabolism</subject><subject>Proteins</subject><subject>Reproducibility of Results</subject><subject>Solanum tuberosum - genetics</subject><subject>Solanum tuberosum - physiology</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc9rFDEYhoNYbK3ePEvAiwfH5ufO5CKUdq1CoUXrOWQyX7ZZZpIxyRT735ultaw9JV--h4e8vAi9o-Qz54qc-O2UGSGCdJK9QEdUMNYQsmpf7t0P0euct4QwzqR6hQ45abu6FEco3CQTsk1-LnECfJ2i86MPG_wD7sCMGa-dA1syjg6fp7hsbgv-WRLk-hLwBQTA6z_zbvZ19gGf-3mMfsDXsZgSd0Qs93M184aq7g06cNUKbx_PY_Tr6_rm7FtzeXXx_ez0srGCstJYAs6YlvTCDhyY6RwMbtUbJVuoMVvJB6lk70AJR41kUnKrRKdcJ0wvgfNj9OXBOy_9BIOFUJIZ9Zz8ZNK9jsbr_zfB3-pNvNMrQTlRbRV8fBSk-HuBXPTks4VxNAHikjWjXSVlq7qKfniGbuOSQo2nGeeMCNbSHfXpgbIp5pzAPX2GEr0rUu8XWfH3-wGe4H_N8b_3Ppq1</recordid><startdate>20190215</startdate><enddate>20190215</enddate><creator>Yang, Xiaohui</creator><creator>Liu, Jie</creator><creator>Xu, Jianfei</creator><creator>Duan, Shaoguang</creator><creator>Wang, Qianru</creator><creator>Li, Guangcun</creator><creator>Jin, Liping</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-3297-0242</orcidid><orcidid>https://orcid.org/0000-0001-9119-3474</orcidid><orcidid>https://orcid.org/0000-0003-2024-9914</orcidid></search><sort><creationdate>20190215</creationdate><title>Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198</title><author>Yang, Xiaohui ; Liu, Jie ; Xu, Jianfei ; Duan, Shaoguang ; Wang, Qianru ; Li, Guangcun ; Jin, Liping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-c0efaa70b4cd3e2a8fedf6ba957e852753d595bfe94f1a52553c9489f84ab5e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abscisic acid</topic><topic>Breeding</topic><topic>Cultivars</topic><topic>Dehydration</topic><topic>Deoxyribonucleic acid</topic><topic>Diploids</topic><topic>Diploidy</topic><topic>DNA</topic><topic>Drought</topic><topic>Drought resistance</topic><topic>Droughts</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene Ontology</topic><topic>Genomes</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Kinases</topic><topic>Molecular modelling</topic><topic>Nucleotide sequence</topic><topic>Osmosis</topic><topic>Phenotype</topic><topic>Phosphotransferase</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Potatoes</topic><topic>Protein kinase</topic><topic>Protein Kinases - metabolism</topic><topic>Proteins</topic><topic>Reproducibility of Results</topic><topic>Solanum tuberosum - genetics</topic><topic>Solanum tuberosum - physiology</topic><topic>Stress, Physiological - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Xiaohui</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Xu, Jianfei</creatorcontrib><creatorcontrib>Duan, Shaoguang</creatorcontrib><creatorcontrib>Wang, Qianru</creatorcontrib><creatorcontrib>Li, Guangcun</creatorcontrib><creatorcontrib>Jin, Liping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Xiaohui</au><au>Liu, Jie</au><au>Xu, Jianfei</au><au>Duan, Shaoguang</au><au>Wang, Qianru</au><au>Li, Guangcun</au><au>Jin, Liping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-02-15</date><risdate>2019</risdate><volume>20</volume><issue>4</issue><spage>852</spage><pages>852-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Potato (
L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to drought stress. Here, a time-course experiment was performed to identify drought resistance response genes in P3-198 under polyethylene glycol (PEG)-induced stress using RNA-sequencing. A total of 1665 differentially expressed genes (DEGs) were specifically identified, and based on gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the transcription factor activity, protein kinase activity, and the plant hormone signal transduction process were significantly enriched. Annotation revealed that these DEGs mainly encode transcription factors, protein kinases, and proteins related to redox regulation, carbohydrate metabolism, and osmotic adjustment. In particular, genes encoding abscisic acid (ABA)-dependent signaling molecules were significantly differentially expressed, which revealed the important roles of the ABA-dependent signaling pathway in the early response of P3-198 to drought stress. Quantitative real-time PCR experimental verification confirmed the differential expression of genes in the drought resistance signaling pathway. Our results provide valuable information for understanding potato drought-resistance mechanisms, and also enrich the gene resources available for drought-resistant potato breeding.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>30781424</pmid><doi>10.3390/ijms20040852</doi><orcidid>https://orcid.org/0000-0002-3297-0242</orcidid><orcidid>https://orcid.org/0000-0001-9119-3474</orcidid><orcidid>https://orcid.org/0000-0003-2024-9914</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Abscisic acid Breeding Cultivars Dehydration Deoxyribonucleic acid Diploids Diploidy DNA Drought Drought resistance Droughts Gene expression Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Gene Ontology Genomes Genotype Genotypes Kinases Molecular modelling Nucleotide sequence Osmosis Phenotype Phosphotransferase Polyethylene Glycols - pharmacology Potatoes Protein kinase Protein Kinases - metabolism Proteins Reproducibility of Results Solanum tuberosum - genetics Solanum tuberosum - physiology Stress, Physiological - genetics Transcription Factors - metabolism |
| title | Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198 |
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