Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform
Cellular activation and inflammation leading to endothelial dysfunction is associated with cardiovascular disease (CVD). We investigated whether a single cell label-free multi parameter optical interrogation system can detect endothelial cell and endothelial progenitor cell (EPC) activation in vitro...
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| Veröffentlicht in: | Scientific reports 2019-03, Vol.9 (1), p.4157-4157, Article 4157 |
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| creator | King, Damien Glynn, MacDara Cindric, Sandra Kernan, David O’Connell, Tríona Hakimjavadi, Roya Kearney, Sinéad Ackermann, Tobias Berbel, Xavier Munoz Llobera, Andreu Simonsen, Ulf Laursen, Britt E. Redmond, Eileen M. Cahill, Paul A. Ducrée, Jens |
| description | Cellular activation and inflammation leading to endothelial dysfunction is associated with cardiovascular disease (CVD). We investigated whether a single cell label-free multi parameter optical interrogation system can detect endothelial cell and endothelial progenitor cell (EPC) activation
in vitro
and
ex vivo
, respectively. Cultured human endothelial cells were exposed to increasing concentrations of tumour necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS) before endothelial activation was validated using fluorescence-activated cell sorting (FACS) analysis of inflammatory marker expression (PECAM-1, E-selectin and ICAM-1). A centrifugal microfluidic system and V-cup array was used to capture individual cells before optical measurement of light scattering, immunocytofluorescence, auto-fluorescence (AF) and cell morphology was determined.
In vitro
, TNF-α promoted specific changes to the refractive index and cell morphology of individual cells concomitant with enhanced photon activity of fluorescently labelled inflammatory markers and increased auto-fluorescence (AF) intensity at three different wavelengths, an effect blocked by inhibition of downstream signalling with Iκβ.
Ex vivo
, there was a significant increase in EPC number and AF intensity of individual EPCs from CVD patients concomitant with enhanced PECAM-1 expression when compared to normal controls. This novel label-free ‘lab on a disc’ (LoaD) platform can successfully detect endothelial activation in response to inflammatory stimuli
in vitro
and
ex vivo
. |
| doi_str_mv | 10.1038/s41598-019-40612-8 |
| format | Article |
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in vitro
and
ex vivo
, respectively. Cultured human endothelial cells were exposed to increasing concentrations of tumour necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS) before endothelial activation was validated using fluorescence-activated cell sorting (FACS) analysis of inflammatory marker expression (PECAM-1, E-selectin and ICAM-1). A centrifugal microfluidic system and V-cup array was used to capture individual cells before optical measurement of light scattering, immunocytofluorescence, auto-fluorescence (AF) and cell morphology was determined.
In vitro
, TNF-α promoted specific changes to the refractive index and cell morphology of individual cells concomitant with enhanced photon activity of fluorescently labelled inflammatory markers and increased auto-fluorescence (AF) intensity at three different wavelengths, an effect blocked by inhibition of downstream signalling with Iκβ.
Ex vivo
, there was a significant increase in EPC number and AF intensity of individual EPCs from CVD patients concomitant with enhanced PECAM-1 expression when compared to normal controls. This novel label-free ‘lab on a disc’ (LoaD) platform can successfully detect endothelial activation in response to inflammatory stimuli
in vitro
and
ex vivo
.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-40612-8</identifier><identifier>PMID: 30858536</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 13/2 ; 13/31 ; 13/62 ; 13/95 ; 14/1 ; 14/10 ; 14/34 ; 14/63 ; 631/80/2373 ; 639/624/1111/55 ; 692/4019/592/75 ; Cardiovascular diseases ; CD31 antigen ; Cell activation ; Cell Shape ; Cytology ; E-selectin ; E-Selectin - genetics ; E-Selectin - metabolism ; Endothelial cells ; Flow cytometry ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Fluorescence ; Human Umbilical Vein Endothelial Cells - cytology ; Human Umbilical Vein Endothelial Cells - drug effects ; Human Umbilical Vein Endothelial Cells - metabolism ; Humanities and Social Sciences ; Humans ; Inflammation ; Intercellular adhesion molecule 1 ; Intercellular Adhesion Molecule-1 - genetics ; Intercellular Adhesion Molecule-1 - metabolism ; Light scattering ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Microfluidics ; Morphology ; multidisciplinary ; Platelet Endothelial Cell Adhesion Molecule-1 - genetics ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; Progenitor cells ; Questioning ; Science ; Science (multidisciplinary) ; Tumor Necrosis Factor-alpha - pharmacology ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Tumors</subject><ispartof>Scientific reports, 2019-03, Vol.9 (1), p.4157-4157, Article 4157</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-318a139294725fb2b5120ba6b83f53163cfade07384e029d66db25cafe84c3ba3</citedby><cites>FETCH-LOGICAL-c474t-318a139294725fb2b5120ba6b83f53163cfade07384e029d66db25cafe84c3ba3</cites><orcidid>0000-0002-5385-6502 ; 0000-0002-2169-7666 ; 0000-0002-6447-5756 ; 0000-0002-0366-1897</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411894/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411894/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30858536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>King, Damien</creatorcontrib><creatorcontrib>Glynn, MacDara</creatorcontrib><creatorcontrib>Cindric, Sandra</creatorcontrib><creatorcontrib>Kernan, David</creatorcontrib><creatorcontrib>O’Connell, Tríona</creatorcontrib><creatorcontrib>Hakimjavadi, Roya</creatorcontrib><creatorcontrib>Kearney, Sinéad</creatorcontrib><creatorcontrib>Ackermann, Tobias</creatorcontrib><creatorcontrib>Berbel, Xavier Munoz</creatorcontrib><creatorcontrib>Llobera, Andreu</creatorcontrib><creatorcontrib>Simonsen, Ulf</creatorcontrib><creatorcontrib>Laursen, Britt E.</creatorcontrib><creatorcontrib>Redmond, Eileen M.</creatorcontrib><creatorcontrib>Cahill, Paul A.</creatorcontrib><creatorcontrib>Ducrée, Jens</creatorcontrib><title>Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cellular activation and inflammation leading to endothelial dysfunction is associated with cardiovascular disease (CVD). We investigated whether a single cell label-free multi parameter optical interrogation system can detect endothelial cell and endothelial progenitor cell (EPC) activation
in vitro
and
ex vivo
, respectively. Cultured human endothelial cells were exposed to increasing concentrations of tumour necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS) before endothelial activation was validated using fluorescence-activated cell sorting (FACS) analysis of inflammatory marker expression (PECAM-1, E-selectin and ICAM-1). A centrifugal microfluidic system and V-cup array was used to capture individual cells before optical measurement of light scattering, immunocytofluorescence, auto-fluorescence (AF) and cell morphology was determined.
In vitro
, TNF-α promoted specific changes to the refractive index and cell morphology of individual cells concomitant with enhanced photon activity of fluorescently labelled inflammatory markers and increased auto-fluorescence (AF) intensity at three different wavelengths, an effect blocked by inhibition of downstream signalling with Iκβ.
Ex vivo
, there was a significant increase in EPC number and AF intensity of individual EPCs from CVD patients concomitant with enhanced PECAM-1 expression when compared to normal controls. This novel label-free ‘lab on a disc’ (LoaD) platform can successfully detect endothelial activation in response to inflammatory stimuli
in vitro
and
ex vivo
.</description><subject>13/106</subject><subject>13/2</subject><subject>13/31</subject><subject>13/62</subject><subject>13/95</subject><subject>14/1</subject><subject>14/10</subject><subject>14/34</subject><subject>14/63</subject><subject>631/80/2373</subject><subject>639/624/1111/55</subject><subject>692/4019/592/75</subject><subject>Cardiovascular diseases</subject><subject>CD31 antigen</subject><subject>Cell activation</subject><subject>Cell Shape</subject><subject>Cytology</subject><subject>E-selectin</subject><subject>E-Selectin - genetics</subject><subject>E-Selectin - metabolism</subject><subject>Endothelial cells</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescence</subject><subject>Human Umbilical Vein Endothelial Cells - cytology</subject><subject>Human Umbilical Vein Endothelial Cells - drug effects</subject><subject>Human Umbilical Vein Endothelial Cells - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Intercellular adhesion molecule 1</subject><subject>Intercellular Adhesion Molecule-1 - genetics</subject><subject>Intercellular Adhesion Molecule-1 - metabolism</subject><subject>Light scattering</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Microfluidics</subject><subject>Morphology</subject><subject>multidisciplinary</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - genetics</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</subject><subject>Progenitor cells</subject><subject>Questioning</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Tumors</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9PFTEUxRujAYJ8ARamiRs3I_07r7MxIU9QkmcgUddNp3PnUdJpn22HhG9vYRDQhd20N-fXc-_NQeiYko-UcHWSBZWdagjtGkFayhr1Ch0wImTDOGOvX7z30VHON6QeyTpBuz20z4mSSvL2AN1tTA--OU8A-Nvsi8NXJpkJCiR8uSvOGo8vQq1S3JriYsBxxGdhiOUavKviqS3udlFcwN9d2HrAa_A-4znXChtcW-AqG_zZZYuvvCljTNNb9GY0PsPR432Ifp6f_Vh_bTaXXy7Wp5vGipUoDafKUN7VyVdMjj3rJWWkN22v-Cg5bbkdzQBkxZUAwrqhbYeeSWtGUMLy3vBD9Gnx3c39BIOFUJLxepfcZNKdjsbpv5XgrvU23upWUKo6UQ0-PBqk-GuGXPRU96gbmgBxzprRjoiuraFU9P0_6E2cU6jrPVCUciJopdhC2RRzTjA-DUOJvg9XL-HqGq5-CFffW797ucbTlz9RVoAvQK5S2EJ67v0f29-jfq_t</recordid><startdate>20190311</startdate><enddate>20190311</enddate><creator>King, Damien</creator><creator>Glynn, MacDara</creator><creator>Cindric, Sandra</creator><creator>Kernan, David</creator><creator>O’Connell, Tríona</creator><creator>Hakimjavadi, Roya</creator><creator>Kearney, Sinéad</creator><creator>Ackermann, Tobias</creator><creator>Berbel, Xavier Munoz</creator><creator>Llobera, Andreu</creator><creator>Simonsen, Ulf</creator><creator>Laursen, Britt E.</creator><creator>Redmond, Eileen M.</creator><creator>Cahill, Paul A.</creator><creator>Ducrée, Jens</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5385-6502</orcidid><orcidid>https://orcid.org/0000-0002-2169-7666</orcidid><orcidid>https://orcid.org/0000-0002-6447-5756</orcidid><orcidid>https://orcid.org/0000-0002-0366-1897</orcidid></search><sort><creationdate>20190311</creationdate><title>Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform</title><author>King, Damien ; Glynn, MacDara ; Cindric, Sandra ; Kernan, David ; O’Connell, Tríona ; Hakimjavadi, Roya ; Kearney, Sinéad ; Ackermann, Tobias ; Berbel, Xavier Munoz ; Llobera, Andreu ; Simonsen, Ulf ; Laursen, Britt E. ; Redmond, Eileen M. ; Cahill, Paul A. ; Ducrée, Jens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-318a139294725fb2b5120ba6b83f53163cfade07384e029d66db25cafe84c3ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>13/106</topic><topic>13/2</topic><topic>13/31</topic><topic>13/62</topic><topic>13/95</topic><topic>14/1</topic><topic>14/10</topic><topic>14/34</topic><topic>14/63</topic><topic>631/80/2373</topic><topic>639/624/1111/55</topic><topic>692/4019/592/75</topic><topic>Cardiovascular diseases</topic><topic>CD31 antigen</topic><topic>Cell activation</topic><topic>Cell Shape</topic><topic>Cytology</topic><topic>E-selectin</topic><topic>E-Selectin - genetics</topic><topic>E-Selectin - metabolism</topic><topic>Endothelial cells</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - instrumentation</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescence</topic><topic>Human Umbilical Vein Endothelial Cells - cytology</topic><topic>Human Umbilical Vein Endothelial Cells - drug effects</topic><topic>Human Umbilical Vein Endothelial Cells - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Intercellular adhesion molecule 1</topic><topic>Intercellular Adhesion Molecule-1 - genetics</topic><topic>Intercellular Adhesion Molecule-1 - metabolism</topic><topic>Light scattering</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Microfluidics</topic><topic>Morphology</topic><topic>multidisciplinary</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - genetics</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</topic><topic>Progenitor cells</topic><topic>Questioning</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>King, Damien</creatorcontrib><creatorcontrib>Glynn, MacDara</creatorcontrib><creatorcontrib>Cindric, Sandra</creatorcontrib><creatorcontrib>Kernan, David</creatorcontrib><creatorcontrib>O’Connell, Tríona</creatorcontrib><creatorcontrib>Hakimjavadi, Roya</creatorcontrib><creatorcontrib>Kearney, Sinéad</creatorcontrib><creatorcontrib>Ackermann, Tobias</creatorcontrib><creatorcontrib>Berbel, Xavier Munoz</creatorcontrib><creatorcontrib>Llobera, Andreu</creatorcontrib><creatorcontrib>Simonsen, Ulf</creatorcontrib><creatorcontrib>Laursen, Britt E.</creatorcontrib><creatorcontrib>Redmond, Eileen M.</creatorcontrib><creatorcontrib>Cahill, Paul A.</creatorcontrib><creatorcontrib>Ducrée, Jens</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>King, Damien</au><au>Glynn, MacDara</au><au>Cindric, Sandra</au><au>Kernan, David</au><au>O’Connell, Tríona</au><au>Hakimjavadi, Roya</au><au>Kearney, Sinéad</au><au>Ackermann, Tobias</au><au>Berbel, Xavier Munoz</au><au>Llobera, Andreu</au><au>Simonsen, Ulf</au><au>Laursen, Britt E.</au><au>Redmond, Eileen M.</au><au>Cahill, Paul A.</au><au>Ducrée, Jens</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-03-11</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>4157</spage><epage>4157</epage><pages>4157-4157</pages><artnum>4157</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cellular activation and inflammation leading to endothelial dysfunction is associated with cardiovascular disease (CVD). We investigated whether a single cell label-free multi parameter optical interrogation system can detect endothelial cell and endothelial progenitor cell (EPC) activation
in vitro
and
ex vivo
, respectively. Cultured human endothelial cells were exposed to increasing concentrations of tumour necrosis factor alpha (TNF-α) or lipopolysaccharide (LPS) before endothelial activation was validated using fluorescence-activated cell sorting (FACS) analysis of inflammatory marker expression (PECAM-1, E-selectin and ICAM-1). A centrifugal microfluidic system and V-cup array was used to capture individual cells before optical measurement of light scattering, immunocytofluorescence, auto-fluorescence (AF) and cell morphology was determined.
In vitro
, TNF-α promoted specific changes to the refractive index and cell morphology of individual cells concomitant with enhanced photon activity of fluorescently labelled inflammatory markers and increased auto-fluorescence (AF) intensity at three different wavelengths, an effect blocked by inhibition of downstream signalling with Iκβ.
Ex vivo
, there was a significant increase in EPC number and AF intensity of individual EPCs from CVD patients concomitant with enhanced PECAM-1 expression when compared to normal controls. This novel label-free ‘lab on a disc’ (LoaD) platform can successfully detect endothelial activation in response to inflammatory stimuli
in vitro
and
ex vivo
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30858536</pmid><doi>10.1038/s41598-019-40612-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5385-6502</orcidid><orcidid>https://orcid.org/0000-0002-2169-7666</orcidid><orcidid>https://orcid.org/0000-0002-6447-5756</orcidid><orcidid>https://orcid.org/0000-0002-0366-1897</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2019-03, Vol.9 (1), p.4157-4157, Article 4157 |
| issn | 2045-2322 2045-2322 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 13/106 13/2 13/31 13/62 13/95 14/1 14/10 14/34 14/63 631/80/2373 639/624/1111/55 692/4019/592/75 Cardiovascular diseases CD31 antigen Cell activation Cell Shape Cytology E-selectin E-Selectin - genetics E-Selectin - metabolism Endothelial cells Flow cytometry Flow Cytometry - instrumentation Flow Cytometry - methods Fluorescence Human Umbilical Vein Endothelial Cells - cytology Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - metabolism Humanities and Social Sciences Humans Inflammation Intercellular adhesion molecule 1 Intercellular Adhesion Molecule-1 - genetics Intercellular Adhesion Molecule-1 - metabolism Light scattering Lipopolysaccharides Lipopolysaccharides - pharmacology Microfluidics Morphology multidisciplinary Platelet Endothelial Cell Adhesion Molecule-1 - genetics Platelet Endothelial Cell Adhesion Molecule-1 - metabolism Progenitor cells Questioning Science Science (multidisciplinary) Tumor Necrosis Factor-alpha - pharmacology Tumor necrosis factor-TNF Tumor necrosis factor-α Tumors |
| title | Label-Free Multi Parameter Optical Interrogation of Endothelial Activation in Single Cells using a Lab on a Disc Platform |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T04%3A05%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Label-Free%20Multi%20Parameter%20Optical%20Interrogation%20of%20Endothelial%20Activation%20in%20Single%20Cells%20using%20a%20Lab%20on%20a%20Disc%20Platform&rft.jtitle=Scientific%20reports&rft.au=King,%20Damien&rft.date=2019-03-11&rft.volume=9&rft.issue=1&rft.spage=4157&rft.epage=4157&rft.pages=4157-4157&rft.artnum=4157&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-019-40612-8&rft_dat=%3Cproquest_pubme%3E2190496038%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2190113041&rft_id=info:pmid/30858536&rfr_iscdi=true |