Amelogenic transcriptome profiling in ameloblast-like cells derived from adult gingival epithelial cells
Dental enamel is the highly mineralized tissue covering the tooth surface and is formed by ameloblasts. Ameloblasts have been known to be impossible to detect in adult tooth because they are shed by apoptosis during enamel maturation and tooth eruption. Owing to these, little was known about appropr...
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| Veröffentlicht in: | Scientific reports 2019-03, Vol.9 (1), p.3736-3736, Article 3736 |
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| creator | Hyun, Sun-Yi Mun, Seyoung Kang, Kyung-Jung Lim, Jong-Chan Kim, Shin-Young Han, Kyudong Jang, Young-Joo |
| description | Dental enamel is the highly mineralized tissue covering the tooth surface and is formed by ameloblasts. Ameloblasts have been known to be impossible to detect in adult tooth because they are shed by apoptosis during enamel maturation and tooth eruption. Owing to these, little was known about appropriate cell surface markers to isolate ameloblast-like cells in tissues. To overcome these problems, epithelial cells were selectively cultivated from the gingival tissues and used as a stem cell source for ameloblastic differentiation. When gingival epithelial cells were treated with a specified concentration of BMP2, BMP4, and TGFβ-1, the expression of ameloblast-specific markers was increased, and both the MAPK and Smad signaling pathways were activated. Gingival epithelial cells differentiated into ameloblast-like cells through epithelial-mesenchymal transition. By RNA-Seq analysis, we reported 20 ameloblast-specific genes associated with cell surface, cell adhesion, and extracellular matrix function. These cell surface markers might be useful for the detection and isolation of ameloblast-like cells from dental tissues. |
| doi_str_mv | 10.1038/s41598-019-40091-x |
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Ameloblasts have been known to be impossible to detect in adult tooth because they are shed by apoptosis during enamel maturation and tooth eruption. Owing to these, little was known about appropriate cell surface markers to isolate ameloblast-like cells in tissues. To overcome these problems, epithelial cells were selectively cultivated from the gingival tissues and used as a stem cell source for ameloblastic differentiation. When gingival epithelial cells were treated with a specified concentration of BMP2, BMP4, and TGFβ-1, the expression of ameloblast-specific markers was increased, and both the MAPK and Smad signaling pathways were activated. Gingival epithelial cells differentiated into ameloblast-like cells through epithelial-mesenchymal transition. By RNA-Seq analysis, we reported 20 ameloblast-specific genes associated with cell surface, cell adhesion, and extracellular matrix function. These cell surface markers might be useful for the detection and isolation of ameloblast-like cells from dental tissues.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-40091-x</identifier><identifier>PMID: 30842534</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/21 ; 13/31 ; 38/39 ; 38/91 ; 631/532/1360 ; 631/532/2118/2074 ; 631/532/2128 ; 96/95 ; Ameloblasts ; Apoptosis ; Cell adhesion ; Cell adhesion & migration ; Cell surface ; Dental enamel ; Epithelial cells ; Extracellular matrix ; Gene expression ; Gingiva ; Humanities and Social Sciences ; MAP kinase ; Mesenchyme ; multidisciplinary ; Ribonucleic acid ; RNA ; Science ; Science (multidisciplinary) ; Smad protein ; Stem cells ; Surface markers ; Teeth</subject><ispartof>Scientific reports, 2019-03, Vol.9 (1), p.3736-3736, Article 3736</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Ameloblasts have been known to be impossible to detect in adult tooth because they are shed by apoptosis during enamel maturation and tooth eruption. Owing to these, little was known about appropriate cell surface markers to isolate ameloblast-like cells in tissues. To overcome these problems, epithelial cells were selectively cultivated from the gingival tissues and used as a stem cell source for ameloblastic differentiation. When gingival epithelial cells were treated with a specified concentration of BMP2, BMP4, and TGFβ-1, the expression of ameloblast-specific markers was increased, and both the MAPK and Smad signaling pathways were activated. Gingival epithelial cells differentiated into ameloblast-like cells through epithelial-mesenchymal transition. By RNA-Seq analysis, we reported 20 ameloblast-specific genes associated with cell surface, cell adhesion, and extracellular matrix function. 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Ameloblasts have been known to be impossible to detect in adult tooth because they are shed by apoptosis during enamel maturation and tooth eruption. Owing to these, little was known about appropriate cell surface markers to isolate ameloblast-like cells in tissues. To overcome these problems, epithelial cells were selectively cultivated from the gingival tissues and used as a stem cell source for ameloblastic differentiation. When gingival epithelial cells were treated with a specified concentration of BMP2, BMP4, and TGFβ-1, the expression of ameloblast-specific markers was increased, and both the MAPK and Smad signaling pathways were activated. Gingival epithelial cells differentiated into ameloblast-like cells through epithelial-mesenchymal transition. By RNA-Seq analysis, we reported 20 ameloblast-specific genes associated with cell surface, cell adhesion, and extracellular matrix function. These cell surface markers might be useful for the detection and isolation of ameloblast-like cells from dental tissues.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30842534</pmid><doi>10.1038/s41598-019-40091-x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5200-7341</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/21 13/31 38/39 38/91 631/532/1360 631/532/2118/2074 631/532/2128 96/95 Ameloblasts Apoptosis Cell adhesion Cell adhesion & migration Cell surface Dental enamel Epithelial cells Extracellular matrix Gene expression Gingiva Humanities and Social Sciences MAP kinase Mesenchyme multidisciplinary Ribonucleic acid RNA Science Science (multidisciplinary) Smad protein Stem cells Surface markers Teeth |
| title | Amelogenic transcriptome profiling in ameloblast-like cells derived from adult gingival epithelial cells |
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