Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy

Förster resonance energy transfer (FRET) has been widely used to design indicators for biomolecules. Conventional FRET-based indicators enable quantitative measurements of analyzes by calculating the ratio between donor and acceptor fluorophores. However, such 'hetero-FRET'-based indicator...

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Veröffentlicht in:	Microscopy 2017-04, Vol.66 (2), p.110-119
Hauptverfasser:	Kim, Sang-Yeob, Arai, Yoshiyuki, Tani, Tomomi, Takatsuka, Hirofumi, Saito, Yoshiharu, Kawashima, Takayuki, Kawakami, Shojiro, Miyawaki, Atsushi, Nagai, Takeharu
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Physiological Phenomena Cells - ultrastructure Fluorescence Polarization - instrumentation Fluorescence Polarization - methods Fluorescence Resonance Energy Transfer - instrumentation Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes Green Fluorescent Proteins HeLa Cells Humans Image Processing, Computer-Assisted - instrumentation Image Processing, Computer-Assisted - methods Luminescent Proteins - metabolism Microscopy, Fluorescence - methods Protein Interaction Mapping - methods
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