Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway
Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell l...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2019-03, Vol.17 (3), p.2242-2246 |
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| creator | Mineda, Ayuka Nishimura, Masato Kagawa, Tomohiro Takiguchi, Eri Kawakita, Takako Abe, Akiko Irahara, Minoru |
| description | Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma. |
| doi_str_mv | 10.3892/etm.2019.7209 |
| format | Article |
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A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2019.7209</identifier><identifier>PMID: 30867708</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Bone cancer ; Cancer ; Cancer therapies ; Cancer treatment ; Care and treatment ; Cell growth ; Cellular signal transduction ; Colorectal cancer ; Development and progression ; Drug dosages ; Embryos ; Genes ; Glycogen ; Glycogen synthesis ; Health aspects ; Immunoglobulins ; Kinases ; Pazopanib ; Polysaccharides ; Prostaglandins ; Prostate ; Proteins ; Resveratrol ; Sarcoma ; Statistical analysis ; Studies ; Trabectedin ; Tumors ; Uterine cancer ; Uterine tumors</subject><ispartof>Experimental and therapeutic medicine, 2019-03, Vol.17 (3), p.2242-2246</ispartof><rights>COPYRIGHT 2019 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2019</rights><rights>Copyright: © Mineda et al. 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-314e2d33aadda8758a5ff8fa09a9790385b647839dd3dc1931ada72ab7251f2b3</citedby><cites>FETCH-LOGICAL-c441t-314e2d33aadda8758a5ff8fa09a9790385b647839dd3dc1931ada72ab7251f2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396019/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396019/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30867708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mineda, Ayuka</creatorcontrib><creatorcontrib>Nishimura, Masato</creatorcontrib><creatorcontrib>Kagawa, Tomohiro</creatorcontrib><creatorcontrib>Takiguchi, Eri</creatorcontrib><creatorcontrib>Kawakita, Takako</creatorcontrib><creatorcontrib>Abe, Akiko</creatorcontrib><creatorcontrib>Irahara, Minoru</creatorcontrib><title>Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.</description><subject>Apoptosis</subject><subject>Bone cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Cancer treatment</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Cellular signal transduction</subject><subject>Colorectal cancer</subject><subject>Development and progression</subject><subject>Drug dosages</subject><subject>Embryos</subject><subject>Genes</subject><subject>Glycogen</subject><subject>Glycogen synthesis</subject><subject>Health aspects</subject><subject>Immunoglobulins</subject><subject>Kinases</subject><subject>Pazopanib</subject><subject>Polysaccharides</subject><subject>Prostaglandins</subject><subject>Prostate</subject><subject>Proteins</subject><subject>Resveratrol</subject><subject>Sarcoma</subject><subject>Statistical analysis</subject><subject>Studies</subject><subject>Trabectedin</subject><subject>Tumors</subject><subject>Uterine cancer</subject><subject>Uterine tumors</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks9rFTEQx4MottQevUrAi5d95sfbTXIRStEqFARRPIbZTfJeym6yJtnKw3--WfqsVkwOSWY-8w0zfBF6ScmGS8Xe2jJtGKFqIxhRT9ApFYo1lND26fFOlKQn6DznG1JX21Ep2-fohBPZCUHkKfr1xeZbm6CkOOK8zHOyOduM5_r2bk34GDAEg30wy1AzMMe5xOwzjg4vxSYfLM6QhjgBHuw4ZtwfKr33vS8-7HDZW_w9FJz9LsC4RmYo-59weIGeORizPT-eZ-jbh_dfLz8215-vPl1eXDfDdktLw-nWMsM5gDEgRSuhdU46IAqUUITLtu-2QnJlDDcDVZyCAcGgF6yljvX8DL27152XfrJmsKEkGPWc_ATpoCN4_TgT_F7v4q3uuOrqdKvAm6NAij8Wm4uefF5bhWDjkjWjivJKdl1FX_-D3sQl1b5XSgoumZDtH2oHo9U-uFj_HVZRfdFWQDLFZaU2_6HqNnbyQwzW-Rp_VNDcFwwp5pyse-iREr0aRlfD6NUwejVM5V_9PZgH-rc9-B28Xr4i</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Mineda, Ayuka</creator><creator>Nishimura, Masato</creator><creator>Kagawa, Tomohiro</creator><creator>Takiguchi, Eri</creator><creator>Kawakita, Takako</creator><creator>Abe, Akiko</creator><creator>Irahara, Minoru</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. 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A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30867708</pmid><doi>10.3892/etm.2019.7209</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Bone cancer Cancer Cancer therapies Cancer treatment Care and treatment Cell growth Cellular signal transduction Colorectal cancer Development and progression Drug dosages Embryos Genes Glycogen Glycogen synthesis Health aspects Immunoglobulins Kinases Pazopanib Polysaccharides Prostaglandins Prostate Proteins Resveratrol Sarcoma Statistical analysis Studies Trabectedin Tumors Uterine cancer Uterine tumors |
| title | Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway |
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