MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer
MiR-494 plays an important role in several types of human cancers, including non-small cell lung cancer (NSCLC). Although the role of miR-494 has been investigated in several studies, the expression profile and underlying mechanism are still poorly understood. In this study, we found that overexpres...
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| Veröffentlicht in: | Scientific reports 2019-02, Vol.9 (1), p.3008, Article 3008 |
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| description | MiR-494 plays an important role in several types of human cancers, including non-small cell lung cancer (NSCLC). Although the role of miR-494 has been investigated in several studies, the expression profile and underlying mechanism are still poorly understood. In this study, we found that overexpression of miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis. By using microarray and Dual luciferase reporter assays, we further showed that caspase-2 (CASP2) is a functional target of miR-494, and the expression of CASP2 is inversely associated with miR-494
in vitro
. In addition, miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis by targeting CASP2. Therefore, our results suggest that miR-494 plays an oncomiR role in NSCLC cells and may be a candidate biomarker for malignant transformation and a therapeutic target of NSCLC. |
| doi_str_mv | 10.1038/s41598-019-39453-2 |
| format | Article |
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in vitro
. In addition, miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis by targeting CASP2. Therefore, our results suggest that miR-494 plays an oncomiR role in NSCLC cells and may be a candidate biomarker for malignant transformation and a therapeutic target of NSCLC.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-39453-2</identifier><identifier>PMID: 30816202</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/105 ; 13/106 ; 13/109 ; 13/2 ; 13/31 ; 13/89 ; 38/39 ; 38/61 ; 38/77 ; 631/45/500 ; 631/67/1612/1350 ; Apoptosis ; Caspase ; Caspase-2 ; Cell proliferation ; Cisplatin ; Colonies ; Humanities and Social Sciences ; Lung cancer ; multidisciplinary ; Non-small cell lung carcinoma ; Science ; Science (multidisciplinary) ; Therapeutic applications</subject><ispartof>Scientific reports, 2019-02, Vol.9 (1), p.3008, Article 3008</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-ff1d0fbfcf7aaf83e8cf8bded36313a8e4fba1e2fb50e9a5152bfded651789c63</citedby><cites>FETCH-LOGICAL-c474t-ff1d0fbfcf7aaf83e8cf8bded36313a8e4fba1e2fb50e9a5152bfded651789c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395740/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395740/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27926,27927,41122,42191,51578,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30816202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qiao</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhao, Mei</creatorcontrib><creatorcontrib>Lin, Hong</creatorcontrib><creatorcontrib>Wang, Wenjie</creatorcontrib><creatorcontrib>Li, Dongdong</creatorcontrib><creatorcontrib>Cui, Wei</creatorcontrib><creatorcontrib>Zhou, Caihong</creatorcontrib><creatorcontrib>Zhong, Jialing</creatorcontrib><creatorcontrib>Huang, Changzhi</creatorcontrib><title>MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>MiR-494 plays an important role in several types of human cancers, including non-small cell lung cancer (NSCLC). Although the role of miR-494 has been investigated in several studies, the expression profile and underlying mechanism are still poorly understood. In this study, we found that overexpression of miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis. By using microarray and Dual luciferase reporter assays, we further showed that caspase-2 (CASP2) is a functional target of miR-494, and the expression of CASP2 is inversely associated with miR-494
in vitro
. In addition, miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis by targeting CASP2. Therefore, our results suggest that miR-494 plays an oncomiR role in NSCLC cells and may be a candidate biomarker for malignant transformation and a therapeutic target of NSCLC.</description><subject>13/1</subject><subject>13/105</subject><subject>13/106</subject><subject>13/109</subject><subject>13/2</subject><subject>13/31</subject><subject>13/89</subject><subject>38/39</subject><subject>38/61</subject><subject>38/77</subject><subject>631/45/500</subject><subject>631/67/1612/1350</subject><subject>Apoptosis</subject><subject>Caspase</subject><subject>Caspase-2</subject><subject>Cell proliferation</subject><subject>Cisplatin</subject><subject>Colonies</subject><subject>Humanities and Social Sciences</subject><subject>Lung cancer</subject><subject>multidisciplinary</subject><subject>Non-small cell lung carcinoma</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Therapeutic applications</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UVtLBCEUligqqj_QQwg9W15n9CWIpRsU3Z_FcXWbmNFNZ4L991nb9aWDHIXvcg5-AOwSfEAwk4eZE6EkwkQhprhgiK6ATYq5QJRRuvrrvQF2cn7GpQRVnKh1sMGwJBXFdBPcXrV3iCsOjR0yNOXAYexjgvMU-zi4BJsFHEyauaENMzg5vr-hsA0wxIByb7oOWldaNxbQmmBd2gZr3nTZ7XzeW-Dx9ORhco4ur88uJseXyPKaD8h7MsW-8dbXxnjJnLReNlM3ZRUjzEjHfWOIo74R2CkjiKCNL3AlSC2VrdgWOFr6zsemd1PrwpBMp-ep7U1a6Gha_RcJ7ZOexVddMSVqjovB_qdBii-jy4N-jmMKZWdNiawxYZjKwqJLlk0x5-T89wSC9XsSepmELknojyQ0LaK937t9S77-vRDYkpALFGYu_cz-x_YNLsiUgQ</recordid><startdate>20190228</startdate><enddate>20190228</enddate><creator>Zhang, Qiao</creator><creator>Li, Yan</creator><creator>Zhao, Mei</creator><creator>Lin, Hong</creator><creator>Wang, Wenjie</creator><creator>Li, Dongdong</creator><creator>Cui, Wei</creator><creator>Zhou, Caihong</creator><creator>Zhong, Jialing</creator><creator>Huang, Changzhi</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20190228</creationdate><title>MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer</title><author>Zhang, Qiao ; Li, Yan ; Zhao, Mei ; Lin, Hong ; Wang, Wenjie ; Li, Dongdong ; Cui, Wei ; Zhou, Caihong ; Zhong, Jialing ; Huang, Changzhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-ff1d0fbfcf7aaf83e8cf8bded36313a8e4fba1e2fb50e9a5152bfded651789c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>13/1</topic><topic>13/105</topic><topic>13/106</topic><topic>13/109</topic><topic>13/2</topic><topic>13/31</topic><topic>13/89</topic><topic>38/39</topic><topic>38/61</topic><topic>38/77</topic><topic>631/45/500</topic><topic>631/67/1612/1350</topic><topic>Apoptosis</topic><topic>Caspase</topic><topic>Caspase-2</topic><topic>Cell proliferation</topic><topic>Cisplatin</topic><topic>Colonies</topic><topic>Humanities and Social Sciences</topic><topic>Lung cancer</topic><topic>multidisciplinary</topic><topic>Non-small cell lung carcinoma</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Therapeutic applications</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qiao</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Zhao, Mei</creatorcontrib><creatorcontrib>Lin, Hong</creatorcontrib><creatorcontrib>Wang, Wenjie</creatorcontrib><creatorcontrib>Li, Dongdong</creatorcontrib><creatorcontrib>Cui, Wei</creatorcontrib><creatorcontrib>Zhou, Caihong</creatorcontrib><creatorcontrib>Zhong, Jialing</creatorcontrib><creatorcontrib>Huang, Changzhi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qiao</au><au>Li, Yan</au><au>Zhao, Mei</au><au>Lin, Hong</au><au>Wang, Wenjie</au><au>Li, Dongdong</au><au>Cui, Wei</au><au>Zhou, Caihong</au><au>Zhong, Jialing</au><au>Huang, Changzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-02-28</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>3008</spage><pages>3008-</pages><artnum>3008</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>MiR-494 plays an important role in several types of human cancers, including non-small cell lung cancer (NSCLC). Although the role of miR-494 has been investigated in several studies, the expression profile and underlying mechanism are still poorly understood. In this study, we found that overexpression of miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis. By using microarray and Dual luciferase reporter assays, we further showed that caspase-2 (CASP2) is a functional target of miR-494, and the expression of CASP2 is inversely associated with miR-494
in vitro
. In addition, miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis by targeting CASP2. Therefore, our results suggest that miR-494 plays an oncomiR role in NSCLC cells and may be a candidate biomarker for malignant transformation and a therapeutic target of NSCLC.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30816202</pmid><doi>10.1038/s41598-019-39453-2</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/105 13/106 13/109 13/2 13/31 13/89 38/39 38/61 38/77 631/45/500 631/67/1612/1350 Apoptosis Caspase Caspase-2 Cell proliferation Cisplatin Colonies Humanities and Social Sciences Lung cancer multidisciplinary Non-small cell lung carcinoma Science Science (multidisciplinary) Therapeutic applications |
| title | MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer |
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