Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations
This study aimed to evaluate the profiles of Streptococcus thermophilus nutrient requirements to guide the design of media for high cell density culturing. The growth kinetics, physiological state, and nutrient requirement profiles of S. thermophilus were analyzed in chemically defined media. The re...
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| description | This study aimed to evaluate the profiles of Streptococcus thermophilus nutrient requirements to guide the design of media for high cell density culturing. The growth kinetics, physiological state, and nutrient requirement profiles of S. thermophilus were analyzed in chemically defined media. The results showed that the intracellular ATP concentration, H+‐ATPase activity, NADH/NAD+, and NH3 concentrations varied with intracellular pH. The nutrient components with the highest amounts required were Leu and Asp; ascorbic acid and p‐amino benzoic acid; K+ and PO43−; and guanine and uracil. The nutrient components with the largest required ratios were Arg, His, and Met; folic acid, cyanocobalamine, biotin, and nicotinic acid; Ca2+ and Mg2+; and guanine and uracil. In this study, different nutrient components were primarily used at different phase. Trp, Tyr, calcium pantothenate, thiamine, guanine, and Mg2+ were mainly used from late‐lag to midexponential phase. Met, Pro, Phe, Ala, Gly, nicotinic acid, and riboflavin were mainly used from midexponential to late‐exponential phase. The highest bioavailabilities of nutrient components were also found at diverse phase. Met, Leu, Ile, Asn, Glu, Lys, Pro, Gly, riboflavin, nicotinic acid, adenine, uracil, inosine, and Ca2+ had the highest bioavailability from late‐lag to midexponential phase. Lactose, Glu, Asp, His, Trp, Cys, Val, Arg, Phe, Ala, Ser, Thr, Tyr, folate and cobalamin, calcium pantothenate, ascorbic acid, thiamine, biotin, p‐amino benzoic acid, vitamin B6, K+, Mg2+, guanine, xanthine, and PO43− had the highest bioavailability from midexponential to late‐exponential phase. This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of S. thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.
This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of Streptococcus thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies. |
| doi_str_mv | 10.1002/mbo3.633 |
| format | Article |
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This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of Streptococcus thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.</description><identifier>ISSN: 2045-8827</identifier><identifier>EISSN: 2045-8827</identifier><identifier>DOI: 10.1002/mbo3.633</identifier><identifier>PMID: 29682906</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Adenosine Triphosphate - analysis ; amino acid ; Ammonia - analysis ; Bacterial Proton-Translocating ATPases - analysis ; Culture Media - chemistry ; Fermentation ; Hydrogen-Ion Concentration ; inosine ; NAD - analysis ; Nutrients - metabolism ; Original ; purine ; pyrimidine ; requirement profiles ; S. thermophilus ; Streptococcus ; Streptococcus thermophilus - chemistry ; Streptococcus thermophilus - growth & development ; Streptococcus thermophilus - metabolism ; vitamin</subject><ispartof>MicrobiologyOpen (Weinheim), 2019-02, Vol.8 (2), p.e00633-n/a</ispartof><rights>2018 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.</rights><rights>2019. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4663-b6a305be9b0f22438383d0794590916e07b7ddb35d3e737f398c183a09fb783</citedby><cites>FETCH-LOGICAL-c4663-b6a305be9b0f22438383d0794590916e07b7ddb35d3e737f398c183a09fb783</cites><orcidid>0000-0002-4158-2524</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391275/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391275/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,1418,11567,27929,27930,45579,45580,46057,46481,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29682906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Gefei</creatorcontrib><creatorcontrib>Qiao, Yali</creatorcontrib><creatorcontrib>Zhang, Yanjiao</creatorcontrib><creatorcontrib>Leng, Cong</creatorcontrib><creatorcontrib>Sun, Jiahui</creatorcontrib><creatorcontrib>Chen, Hongyu</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Aili</creatorcontrib><creatorcontrib>Feng, Zhen</creatorcontrib><title>Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations</title><title>MicrobiologyOpen (Weinheim)</title><addtitle>Microbiologyopen</addtitle><description>This study aimed to evaluate the profiles of Streptococcus thermophilus nutrient requirements to guide the design of media for high cell density culturing. The growth kinetics, physiological state, and nutrient requirement profiles of S. thermophilus were analyzed in chemically defined media. The results showed that the intracellular ATP concentration, H+‐ATPase activity, NADH/NAD+, and NH3 concentrations varied with intracellular pH. The nutrient components with the highest amounts required were Leu and Asp; ascorbic acid and p‐amino benzoic acid; K+ and PO43−; and guanine and uracil. The nutrient components with the largest required ratios were Arg, His, and Met; folic acid, cyanocobalamine, biotin, and nicotinic acid; Ca2+ and Mg2+; and guanine and uracil. In this study, different nutrient components were primarily used at different phase. Trp, Tyr, calcium pantothenate, thiamine, guanine, and Mg2+ were mainly used from late‐lag to midexponential phase. Met, Pro, Phe, Ala, Gly, nicotinic acid, and riboflavin were mainly used from midexponential to late‐exponential phase. The highest bioavailabilities of nutrient components were also found at diverse phase. Met, Leu, Ile, Asn, Glu, Lys, Pro, Gly, riboflavin, nicotinic acid, adenine, uracil, inosine, and Ca2+ had the highest bioavailability from late‐lag to midexponential phase. Lactose, Glu, Asp, His, Trp, Cys, Val, Arg, Phe, Ala, Ser, Thr, Tyr, folate and cobalamin, calcium pantothenate, ascorbic acid, thiamine, biotin, p‐amino benzoic acid, vitamin B6, K+, Mg2+, guanine, xanthine, and PO43− had the highest bioavailability from midexponential to late‐exponential phase. This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of S. thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.
This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of Streptococcus thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.</description><subject>Adenosine Triphosphate - analysis</subject><subject>amino acid</subject><subject>Ammonia - analysis</subject><subject>Bacterial Proton-Translocating ATPases - analysis</subject><subject>Culture Media - chemistry</subject><subject>Fermentation</subject><subject>Hydrogen-Ion Concentration</subject><subject>inosine</subject><subject>NAD - analysis</subject><subject>Nutrients - metabolism</subject><subject>Original</subject><subject>purine</subject><subject>pyrimidine</subject><subject>requirement profiles</subject><subject>S. thermophilus</subject><subject>Streptococcus</subject><subject>Streptococcus thermophilus - chemistry</subject><subject>Streptococcus thermophilus - growth & development</subject><subject>Streptococcus thermophilus - metabolism</subject><subject>vitamin</subject><issn>2045-8827</issn><issn>2045-8827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kd1qFjEQhoMottSCVyABTzzZNj-7yeZE0KKt8LUVKgiehCSb9UvZ3WyTrNKzXkKvsVfifPTHVjADyYR5eWaSF6HXlOxRQtj-aCPfE5w_Q9uM1E3Vtkw-f5Rvod2czwksSZio6Uu0xZRomSJiG81fU-zD4DOOPT4ryc8luujcknFZ-zTGeR0GuByf3Fxd_1h9hx164mkpKfip4OQvlpD8CHnGYcIuTiXFYfAdno-wNcWtcQ8cqJsS4pRfoRe9GbLfvTt30NnnT98OjqrV6eGXgw-rytVC8MoKw0ljvbKkZ6zmLURHpKobRRQVnkgru87ypuNectlz1TrackNUb2XLd9D7W-q82NF3DtonM-g5hdGkSx1N0E8rU1jrn_GXFlxRJhsAvLsDpHix-Fz0GLLzw2AmH5esGYE_bIhoKEjf_iM9j0ua4HGa0VYQiJr9BboUc06-fxiGEr3xUW98hP4cpG8eD_8gvHcNBNWt4Dc4d_lfkD7-eMo3wD94F6mF</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Liu, Gefei</creator><creator>Qiao, Yali</creator><creator>Zhang, Yanjiao</creator><creator>Leng, Cong</creator><creator>Sun, Jiahui</creator><creator>Chen, Hongyu</creator><creator>Zhang, Yan</creator><creator>Li, Aili</creator><creator>Feng, Zhen</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4158-2524</orcidid></search><sort><creationdate>201902</creationdate><title>Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations</title><author>Liu, Gefei ; Qiao, Yali ; Zhang, Yanjiao ; Leng, Cong ; Sun, Jiahui ; Chen, Hongyu ; Zhang, Yan ; Li, Aili ; Feng, Zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4663-b6a305be9b0f22438383d0794590916e07b7ddb35d3e737f398c183a09fb783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine Triphosphate - analysis</topic><topic>amino acid</topic><topic>Ammonia - analysis</topic><topic>Bacterial Proton-Translocating ATPases - analysis</topic><topic>Culture Media - chemistry</topic><topic>Fermentation</topic><topic>Hydrogen-Ion Concentration</topic><topic>inosine</topic><topic>NAD - analysis</topic><topic>Nutrients - metabolism</topic><topic>Original</topic><topic>purine</topic><topic>pyrimidine</topic><topic>requirement profiles</topic><topic>S. thermophilus</topic><topic>Streptococcus</topic><topic>Streptococcus thermophilus - chemistry</topic><topic>Streptococcus thermophilus - growth & development</topic><topic>Streptococcus thermophilus - metabolism</topic><topic>vitamin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Gefei</creatorcontrib><creatorcontrib>Qiao, Yali</creatorcontrib><creatorcontrib>Zhang, Yanjiao</creatorcontrib><creatorcontrib>Leng, Cong</creatorcontrib><creatorcontrib>Sun, Jiahui</creatorcontrib><creatorcontrib>Chen, Hongyu</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Li, Aili</creatorcontrib><creatorcontrib>Feng, Zhen</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>MicrobiologyOpen (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Gefei</au><au>Qiao, Yali</au><au>Zhang, Yanjiao</au><au>Leng, Cong</au><au>Sun, Jiahui</au><au>Chen, Hongyu</au><au>Zhang, Yan</au><au>Li, Aili</au><au>Feng, Zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations</atitle><jtitle>MicrobiologyOpen (Weinheim)</jtitle><addtitle>Microbiologyopen</addtitle><date>2019-02</date><risdate>2019</risdate><volume>8</volume><issue>2</issue><spage>e00633</spage><epage>n/a</epage><pages>e00633-n/a</pages><issn>2045-8827</issn><eissn>2045-8827</eissn><abstract>This study aimed to evaluate the profiles of Streptococcus thermophilus nutrient requirements to guide the design of media for high cell density culturing. The growth kinetics, physiological state, and nutrient requirement profiles of S. thermophilus were analyzed in chemically defined media. The results showed that the intracellular ATP concentration, H+‐ATPase activity, NADH/NAD+, and NH3 concentrations varied with intracellular pH. The nutrient components with the highest amounts required were Leu and Asp; ascorbic acid and p‐amino benzoic acid; K+ and PO43−; and guanine and uracil. The nutrient components with the largest required ratios were Arg, His, and Met; folic acid, cyanocobalamine, biotin, and nicotinic acid; Ca2+ and Mg2+; and guanine and uracil. In this study, different nutrient components were primarily used at different phase. Trp, Tyr, calcium pantothenate, thiamine, guanine, and Mg2+ were mainly used from late‐lag to midexponential phase. Met, Pro, Phe, Ala, Gly, nicotinic acid, and riboflavin were mainly used from midexponential to late‐exponential phase. The highest bioavailabilities of nutrient components were also found at diverse phase. Met, Leu, Ile, Asn, Glu, Lys, Pro, Gly, riboflavin, nicotinic acid, adenine, uracil, inosine, and Ca2+ had the highest bioavailability from late‐lag to midexponential phase. Lactose, Glu, Asp, His, Trp, Cys, Val, Arg, Phe, Ala, Ser, Thr, Tyr, folate and cobalamin, calcium pantothenate, ascorbic acid, thiamine, biotin, p‐amino benzoic acid, vitamin B6, K+, Mg2+, guanine, xanthine, and PO43− had the highest bioavailability from midexponential to late‐exponential phase. This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of S. thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.
This study elucidated the nutrient requirement profiles with culture time and biomass at various average growth rates during the growth of Streptococcus thermophilus. The present results will help to formulate complex media for high cell density cultivation and provide the theoretical basis for S. thermophilus feeding strategies.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>29682906</pmid><doi>10.1002/mbo3.633</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4158-2524</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Online Library (Open Access Collection); PubMed Central |
| subjects | Adenosine Triphosphate - analysis amino acid Ammonia - analysis Bacterial Proton-Translocating ATPases - analysis Culture Media - chemistry Fermentation Hydrogen-Ion Concentration inosine NAD - analysis Nutrients - metabolism Original purine pyrimidine requirement profiles S. thermophilus Streptococcus Streptococcus thermophilus - chemistry Streptococcus thermophilus - growth & development Streptococcus thermophilus - metabolism vitamin |
| title | Profiles of Streptococcus thermophilus MN‐ZLW‐002 nutrient requirements in controlled pH batch fermentations |
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