Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana

The cause of acute encephalitis/meningoencephalitis in pediatric patients remains often unexplained despite extensive investigations for large panel of pathogens. To explore a possible viral implication, we investigated the virome of cerebrospinal fluid specimens of 70 febrile pediatric inpatients w...

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Veröffentlicht in:Scientific reports 2019-02, Vol.9 (1), p.2360-2360, Article 2360
Hauptverfasser: Eibach, Daniel, Hogan, Benedikt, Sarpong, Nimako, Winter, Doris, Struck, Nicole S., Adu-Sarkodie, Yaw, Owusu-Dabo, Ellis, Schmidt-Chanasit, Jonas, May, Jürgen, Cadar, Daniel
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container_title Scientific reports
container_volume 9
creator Eibach, Daniel
Hogan, Benedikt
Sarpong, Nimako
Winter, Doris
Struck, Nicole S.
Adu-Sarkodie, Yaw
Owusu-Dabo, Ellis
Schmidt-Chanasit, Jonas
May, Jürgen
Cadar, Daniel
description The cause of acute encephalitis/meningoencephalitis in pediatric patients remains often unexplained despite extensive investigations for large panel of pathogens. To explore a possible viral implication, we investigated the virome of cerebrospinal fluid specimens of 70 febrile pediatric inpatients with clinical compatible encephalitis/meningoencephalitis. Using viral metagenomics, we detected and genetically characterized three novel human Torque teno mini virus (TTMV) species (TTMV-G1-3). Phylogenetically, TTMV-G1-3 clustered in three novel monophyletic lineages within genus Betatorquevirus of the Anelloviridae family. TTMV-G1-3 were highly prevalent in diseased children, but absent in the healthy cohort which may indicate an association of TTMV species with febrile illness. With 2/3 detected malaria co-infection, it remains unclear if these novel anellovirus species are causative agents or increase disease severity by interaction with malaria parasites. The presence of the viruses 28 days after initiating antimalarial and/or antibiotic treatment suggests a still active viral infection likely as effect of parasitic and/or bacterial co-infection that may have initiated a modulated immune system environment for viral replication or a defective virus clearance. This study increases the current knowledge on the genetic diversity of TTMV and strengthens that human anelloviruses can be considered as biomarkers for strong perturbations of the immune system in certain pathological conditions.
doi_str_mv 10.1038/s41598-019-38975-z
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subjects 692/699/255
692/699/255/1638
692/699/255/2514
Cerebrospinal fluid
Encephalitis
Genetic diversity
Humanities and Social Sciences
Immune clearance
Immune system
Infections
Malaria
Meningitis
Meningoencephalitis
multidisciplinary
Parasites
Pediatrics
Phylogeny
Science
Science (multidisciplinary)
Species
Vector-borne diseases
Viral infections
title Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana
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