Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone
Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in r...
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| Veröffentlicht in: | BMC microbiology 2019-02, Vol.19 (1), p.45, Article 45 |
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| creator | Abril, Deisy Marquez-Ortiz, Ricaurte Alejandro Castro-Cardozo, Betsy Moncayo-Ortiz, José Ignacio Olarte Escobar, Narda María Corredor Rozo, Zayda Lorena Reyes, Niradiz Tovar, Catalina Sánchez, Héctor Fabio Castellanos, Jaime Guaca-González, Yina Marcela Llanos-Uribe, Carmen Elisa Vanegas Gómez, Natasha Escobar-Pérez, Javier |
| description | Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla.sub.KPC-2-positive P. aeruginosa ST235 in Colombia. In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla.sub.VIM and bla.sub.KPC-2 genes, respectively. The four bla.sub.KPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2")-Ia, two Tn402-like with ant(3")-Ia and bla.sub.OXA-2 and two Tn4401b with bla.sub.KPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla.sub.KPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism. |
| doi_str_mv | 10.1186/s12866-019-1418-6 |
| format | Article |
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Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla.sub.KPC-2-positive P. aeruginosa ST235 in Colombia. In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla.sub.VIM and bla.sub.KPC-2 genes, respectively. The four bla.sub.KPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2")-Ia, two Tn402-like with ant(3")-Ia and bla.sub.OXA-2 and two Tn4401b with bla.sub.KPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla.sub.KPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/s12866-019-1418-6</identifier><identifier>PMID: 30786858</identifier><language>eng</language><publisher>London: BioMed Central Ltd</publisher><subject>Antibiotics ; Beta lactamases ; Carbapenems ; Chromosome rearrangements ; Cities and towns ; Cloning ; DNA sequencing ; Enzymes ; Gene sequencing ; Genes ; Genetic aspects ; Genomes ; Genomic islands ; Genomics ; Infection ; Infections ; Insertion ; Intelligence gathering ; Intensive care ; Medical research ; Pathogens ; Plastic properties ; Plasticity ; Pseudomonas aeruginosa ; Surveillance ; Transposition ; Transposons</subject><ispartof>BMC microbiology, 2019-02, Vol.19 (1), p.45, Article 45</ispartof><rights>COPYRIGHT 2019 BioMed Central Ltd.</rights><rights>Copyright © 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s). 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3506-b5981e49cc032b2a7c83d593e54e911783e45ee70600c4ba7193f68897593b783</citedby><cites>FETCH-LOGICAL-c3506-b5981e49cc032b2a7c83d593e54e911783e45ee70600c4ba7193f68897593b783</cites><orcidid>0000-0002-0432-6978</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381643/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381643/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Abril, Deisy</creatorcontrib><creatorcontrib>Marquez-Ortiz, Ricaurte Alejandro</creatorcontrib><creatorcontrib>Castro-Cardozo, Betsy</creatorcontrib><creatorcontrib>Moncayo-Ortiz, José Ignacio</creatorcontrib><creatorcontrib>Olarte Escobar, Narda María</creatorcontrib><creatorcontrib>Corredor Rozo, Zayda Lorena</creatorcontrib><creatorcontrib>Reyes, Niradiz</creatorcontrib><creatorcontrib>Tovar, Catalina</creatorcontrib><creatorcontrib>Sánchez, Héctor Fabio</creatorcontrib><creatorcontrib>Castellanos, Jaime</creatorcontrib><creatorcontrib>Guaca-González, Yina Marcela</creatorcontrib><creatorcontrib>Llanos-Uribe, Carmen Elisa</creatorcontrib><creatorcontrib>Vanegas Gómez, Natasha</creatorcontrib><creatorcontrib>Escobar-Pérez, Javier</creatorcontrib><title>Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone</title><title>BMC microbiology</title><description>Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla.sub.KPC-2-positive P. aeruginosa ST235 in Colombia. In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla.sub.VIM and bla.sub.KPC-2 genes, respectively. The four bla.sub.KPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2")-Ia, two Tn402-like with ant(3")-Ia and bla.sub.OXA-2 and two Tn4401b with bla.sub.KPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla.sub.KPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.</description><subject>Antibiotics</subject><subject>Beta lactamases</subject><subject>Carbapenems</subject><subject>Chromosome rearrangements</subject><subject>Cities and towns</subject><subject>Cloning</subject><subject>DNA sequencing</subject><subject>Enzymes</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Genomic islands</subject><subject>Genomics</subject><subject>Infection</subject><subject>Infections</subject><subject>Insertion</subject><subject>Intelligence gathering</subject><subject>Intensive care</subject><subject>Medical research</subject><subject>Pathogens</subject><subject>Plastic properties</subject><subject>Plasticity</subject><subject>Pseudomonas 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plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone</title><author>Abril, Deisy ; Marquez-Ortiz, Ricaurte Alejandro ; Castro-Cardozo, Betsy ; Moncayo-Ortiz, José Ignacio ; Olarte Escobar, Narda María ; Corredor Rozo, Zayda Lorena ; Reyes, Niradiz ; Tovar, Catalina ; Sánchez, Héctor Fabio ; Castellanos, Jaime ; Guaca-González, Yina Marcela ; Llanos-Uribe, Carmen Elisa ; Vanegas Gómez, Natasha ; Escobar-Pérez, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3506-b5981e49cc032b2a7c83d593e54e911783e45ee70600c4ba7193f68897593b783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Beta lactamases</topic><topic>Carbapenems</topic><topic>Chromosome rearrangements</topic><topic>Cities and towns</topic><topic>Cloning</topic><topic>DNA sequencing</topic><topic>Enzymes</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Genomic islands</topic><topic>Genomics</topic><topic>Infection</topic><topic>Infections</topic><topic>Insertion</topic><topic>Intelligence gathering</topic><topic>Intensive care</topic><topic>Medical research</topic><topic>Pathogens</topic><topic>Plastic properties</topic><topic>Plasticity</topic><topic>Pseudomonas aeruginosa</topic><topic>Surveillance</topic><topic>Transposition</topic><topic>Transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abril, Deisy</creatorcontrib><creatorcontrib>Marquez-Ortiz, Ricaurte Alejandro</creatorcontrib><creatorcontrib>Castro-Cardozo, Betsy</creatorcontrib><creatorcontrib>Moncayo-Ortiz, José Ignacio</creatorcontrib><creatorcontrib>Olarte Escobar, Narda María</creatorcontrib><creatorcontrib>Corredor Rozo, Zayda Lorena</creatorcontrib><creatorcontrib>Reyes, Niradiz</creatorcontrib><creatorcontrib>Tovar, 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microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abril, Deisy</au><au>Marquez-Ortiz, Ricaurte Alejandro</au><au>Castro-Cardozo, Betsy</au><au>Moncayo-Ortiz, José Ignacio</au><au>Olarte Escobar, Narda María</au><au>Corredor Rozo, Zayda Lorena</au><au>Reyes, Niradiz</au><au>Tovar, Catalina</au><au>Sánchez, Héctor Fabio</au><au>Castellanos, Jaime</au><au>Guaca-González, Yina Marcela</au><au>Llanos-Uribe, Carmen Elisa</au><au>Vanegas Gómez, Natasha</au><au>Escobar-Pérez, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone</atitle><jtitle>BMC microbiology</jtitle><date>2019-02-20</date><risdate>2019</risdate><volume>19</volume><issue>1</issue><spage>45</spage><pages>45-</pages><artnum>45</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla.sub.KPC-2-positive P. aeruginosa ST235 in Colombia. In a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla.sub.VIM and bla.sub.KPC-2 genes, respectively. The four bla.sub.KPC-2-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241 bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2")-Ia, two Tn402-like with ant(3")-Ia and bla.sub.OXA-2 and two Tn4401b with bla.sub.KPC-2. All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla.sub.KPC-2 were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background. This is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.</abstract><cop>London</cop><pub>BioMed Central Ltd</pub><pmid>30786858</pmid><doi>10.1186/s12866-019-1418-6</doi><orcidid>https://orcid.org/0000-0002-0432-6978</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Beta lactamases Carbapenems Chromosome rearrangements Cities and towns Cloning DNA sequencing Enzymes Gene sequencing Genes Genetic aspects Genomes Genomic islands Genomics Infection Infections Insertion Intelligence gathering Intensive care Medical research Pathogens Plastic properties Plasticity Pseudomonas aeruginosa Surveillance Transposition Transposons |
| title | Genome plasticity favours double chromosomal Tn4401b-blaKPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone |
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