Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform
We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2019-01, Vol.55 (6), p.806-809 |
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| container_end_page | 809 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Lim, Samuel Jung, Gi Ahn Muckom, Riya J Glover, Dominic J Clark, Douglas S |
| description | We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible. |
| doi_str_mv | 10.1039/c8cc08720b |
| format | Article |
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Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.</description><identifier>ISSN: 1359-7345</identifier><identifier>ISSN: 1364-548X</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c8cc08720b</identifier><identifier>PMID: 30574651</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Archaeal Proteins - chemistry ; Archaeal Proteins - metabolism ; Biocompatibility ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Cell Survival - drug effects ; Chemical synthesis ; Crosslinking ; Human Embryonic Stem Cells - cytology ; Human Embryonic Stem Cells - metabolism ; Humans ; Hydrogels ; Hydrogels - chemistry ; Immobilization ; Immobilized Proteins - chemistry ; Mechanical properties ; Methanocaldococcus - metabolism ; Microscopy, Confocal ; Molecular Chaperones - chemistry ; Molecular Chaperones - metabolism ; Organic chemistry ; Polymers ; Polymers - chemistry ; Proteins ; Proteins - chemistry ; Proteins - metabolism</subject><ispartof>Chemical communications (Cambridge, England), 2019-01, Vol.55 (6), p.806-809</ispartof><rights>Copyright Royal Society of Chemistry 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-a83b6b3e064b867191f3e91d7b5258c052b5ff1cdc3e79ba7cfed467aba387ff3</citedby><cites>FETCH-LOGICAL-c472t-a83b6b3e064b867191f3e91d7b5258c052b5ff1cdc3e79ba7cfed467aba387ff3</cites><orcidid>0000-0002-5373-285X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30574651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Samuel</creatorcontrib><creatorcontrib>Jung, Gi Ahn</creatorcontrib><creatorcontrib>Muckom, Riya J</creatorcontrib><creatorcontrib>Glover, Dominic J</creatorcontrib><creatorcontrib>Clark, Douglas S</creatorcontrib><title>Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. 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The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.</description><subject>Archaeal Proteins - chemistry</subject><subject>Archaeal Proteins - metabolism</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Chemical synthesis</subject><subject>Crosslinking</subject><subject>Human Embryonic Stem Cells - cytology</subject><subject>Human Embryonic Stem Cells - metabolism</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Immobilization</subject><subject>Immobilized Proteins - chemistry</subject><subject>Mechanical properties</subject><subject>Methanocaldococcus - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Molecular Chaperones - chemistry</subject><subject>Molecular Chaperones - metabolism</subject><subject>Organic chemistry</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><issn>1359-7345</issn><issn>1364-548X</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV9L3jAUxoNM5p_txg8ghd2IUE2apElvBq682wTBG4XdhSRN-kbSpCbt4N2nt1UnbufmHHh-PDycB4ATBC8QxM2l5lpDziqo9sAhwjUpKeG_Pqw3bUqGCT0ARzk_wGUQ5R_BAYaUkZqiQ-A2oXfBmORCXygXY5q2sY9B-mJMcTIulGP0u8GkYrtTyXXL6lLsjS9kLmRh56An954v3DBE5bz7I1ehGL2cbEzDJ7Bvpc_m8-s-BvffN3ftz_Lm9sd1e3VTasKqqZQcq1phA2uieM1Qgyw2DeqYohXlGtJKUWuR7jQ2rFGSaWs6UjOpJObMWnwMvr74jrMaTKdNmJL0YkxukGknonTiXyW4rejjb1FjBgmrF4OzV4MUH2eTJzG4rI33Mpg4Z1Eh2jS8gnhFv_yHPsQ5Lc9YqSU8RrghC3X-QukUc07GvoVBUKwNipa37XOD3xb49H38N_RvZfgJeemacQ</recordid><startdate>20190115</startdate><enddate>20190115</enddate><creator>Lim, Samuel</creator><creator>Jung, Gi Ahn</creator><creator>Muckom, Riya J</creator><creator>Glover, Dominic J</creator><creator>Clark, Douglas S</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5373-285X</orcidid></search><sort><creationdate>20190115</creationdate><title>Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform</title><author>Lim, Samuel ; 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Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>30574651</pmid><doi>10.1039/c8cc08720b</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-5373-285X</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2019-01, Vol.55 (6), p.806-809 |
| issn | 1359-7345 1364-548X 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Archaeal Proteins - chemistry Archaeal Proteins - metabolism Biocompatibility Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Cell Survival - drug effects Chemical synthesis Crosslinking Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Hydrogels Hydrogels - chemistry Immobilization Immobilized Proteins - chemistry Mechanical properties Methanocaldococcus - metabolism Microscopy, Confocal Molecular Chaperones - chemistry Molecular Chaperones - metabolism Organic chemistry Polymers Polymers - chemistry Proteins Proteins - chemistry Proteins - metabolism |
| title | Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform |
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