Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology
Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopo...
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| Veröffentlicht in: | Scientific reports 2019-02, Vol.9 (1), p.1714-1714, Article 1714 |
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| creator | Peserico, Alessia Marcacci, Maurilia Malatesta, Daniela Di Domenico, Marco Pratelli, Annamaria Mangone, Iolanda D’Alterio, Nicola Pizzurro, Federica Cirone, Francesco Zaccaria, Guendalina Cammà, Cesare Lorusso, Alessio |
| description | Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis. |
| doi_str_mv | 10.1038/s41598-018-37497-4 |
| format | Article |
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| subjects | 13/106 13/51 45 45/23 45/47 45/77 631/326/2521 631/326/421 Acids Antigens Binding sites Canine distemper Diagnosis Disease prevention Dogs Epidemics Genomes Humanities and Social Sciences Identification Immunohistochemistry Infections Infectious diseases multidisciplinary Nature Nucleic acids Pathogens Phylogenetics Proteins Ribonucleic acid RNA Science Science (multidisciplinary) Viruses |
| title | Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology |
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