Engineered CRISPR-Cas9 nuclease with expanded targeting space

The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-editing tool. However, the widely used Cas9 enzyme (SpCas9) requires an NGG protospacer adjacent motif (PAM) for target recognition, thereby restricting the targetable genomic loci. Here, we report a rationally engineer...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2018-09, Vol.361 (6408), p.1259-1262
Hauptverfasser:	Nishimasu, Hiroshi, Shi, Xi, Ishiguro, Soh, Gao, Linyi, Hirano, Seiichi, Okazaki, Sae, Noda, Taichi, Abudayyeh, Omar O, Gootenberg, Jonathan S, Mori, Hideto, Oura, Seiya, Holmes, Benjamin, Tanaka, Mamoru, Seki, Motoaki, Hirano, Hisato, Aburatani, Hiroyuki, Ishitani, Ryuichiro, Ikawa, Masahito, Yachie, Nozomu, Zhang, Feng, Nureki, Osamu
Format:	Artikel
Sprache:	eng
Schlagworte:	Activation-induced cytidine deaminase Bacterial Proteins - chemistry Bacterial Proteins - genetics CRISPR CRISPR-Associated Protein 9 CRISPR-Cas Systems Crystal structure Crystallography, X-Ray Deoxyribonucleic acid DNA Endonuclease Endonucleases - chemistry Endonucleases - genetics Enzymes Gene Editing Genome editing Genomes HEK293 Cells Humans Literary Devices Nuclease Nucleotide sequence Protein Engineering Ribonucleic acid RNA Streptococcus pyogenes Target recognition
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