A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1
Testis‐expressed X‐linked genes typically evolve rapidly. Here, we report on a testis‐expressed X‐linked microRNA (miRNA) cluster that despite rapid alterations in sequence has retained its position in the Fragile‐X region of the X chromosome in placental mammals. Surprisingly, the miRNAs encoded by...
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creator | Ramaiah, Madhuvanthi Tan, Kun Plank, Terra‐Dawn M Song, Hye‐Won Chousal, Jennifer N Jones, Samantha Shum, Eleen Y Sheridan, Steven D Peterson, Kevin J Gromoll, Jörg Haggarty, Stephen J Cook‐Andersen, Heidi Wilkinson, Miles F |
description | Testis‐expressed X‐linked genes typically evolve rapidly. Here, we report on a testis‐expressed X‐linked microRNA (miRNA) cluster that despite rapid alterations in sequence has retained its position in the Fragile‐X region of the X chromosome in placental mammals. Surprisingly, the miRNAs encoded by this cluster (
Fx‐mir
) have a predilection for targeting the immediately adjacent gene,
Fmr1
, an unexpected finding given that miRNAs usually act
in trans
, not
in cis
. Robust repression of
Fmr1
is conferred by combinations of
Fx‐mir
miRNAs induced in Sertoli cells (SCs) during postnatal development when they terminate proliferation. Physiological significance is suggested by the finding that FMRP, the protein product of
Fmr1
, is downregulated when
Fx‐mir
miRNAs are induced, and that FMRP loss causes SC hyperproliferation and spermatogenic defects.
Fx‐mir
miRNAs not only regulate the expression of FMRP, but also regulate the expression of eIF4E and CYFIP1, which together with FMRP form a translational regulatory complex. Our results support a model in which
Fx‐mir
family members act cooperatively to regulate the translation of batteries of mRNAs in a developmentally regulated manner in SCs.
Synopsis
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.
A large X‐linked miRNA cluster (
Fx‐mir
) is functionally characterized.
Members of
Fx‐mir
have a predilection for targeting the immediately adjacent gene
Fmr1
.
Fx‐mir
miRNAs are postnatally induced in Sertoli cells and act additively to repress
Fmr1
.
The
Fx‐mir
cluster is rapidly evolving in sequence but its ability to target
Fmr1
is conserved.
Graphical Abstract
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1. |
doi_str_mv | 10.15252/embr.201846566 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6362356</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2159986059</sourcerecordid><originalsourceid>FETCH-LOGICAL-p3866-4d0bd3037129f6edb96d1c94258d257e64c0f3a4b7319b7232ec3f383e745dfb3</originalsourceid><addsrcrecordid>eNp1kU9PFDEYxhuCAVw9cyNNuHhZ7J9pO-VgshBWTRZNNpoQLk1n-s5QMv9oZxRufAQ_o5_EWXdd0MRT27zP-8vT50HokJITKphgb6HOwgkjNE2kkHIHHdBE6imnKt3d3BmjV_voZYy3hBChVbqH9jkRigsmD9D1DNc-D-3y0wzn1RB7CNg3uL8BPA-29BX8fPxxhQOUvm0w3HcBYgSH3RB8U-LYQaht35bQQPQR9zaU0Ec8v1zSV-hFYasIrzfnBH2dX3w5_zBdfH7_8Xy2mHY8lXKaOJI5TriiTBcSXKalo7lOmEgdEwpkkpOC2yRTnOpMMc4g5wVPOahEuCLjE_Ruze2GrAaXQ9MHW5ku-NqGB9Nab_6eNP7GlO03I7lkXMgR8GYDCO3dALE3tY85VJVtoB2iYVRoncoxvFF6_I_0th1CM35vVCmhtFBsBTx67mhr5U_so-B0Lfg-BvywnVNifrdqVq2abavm4vJsuX2Ny2S9HLtVBxCePPwHwH8BorenGg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2175795726</pqid></control><display><type>article</type><title>A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Springer Nature OA Free Journals</source><creator>Ramaiah, Madhuvanthi ; Tan, Kun ; Plank, Terra‐Dawn M ; Song, Hye‐Won ; Chousal, Jennifer N ; Jones, Samantha ; Shum, Eleen Y ; Sheridan, Steven D ; Peterson, Kevin J ; Gromoll, Jörg ; Haggarty, Stephen J ; Cook‐Andersen, Heidi ; Wilkinson, Miles F</creator><creatorcontrib>Ramaiah, Madhuvanthi ; Tan, Kun ; Plank, Terra‐Dawn M ; Song, Hye‐Won ; Chousal, Jennifer N ; Jones, Samantha ; Shum, Eleen Y ; Sheridan, Steven D ; Peterson, Kevin J ; Gromoll, Jörg ; Haggarty, Stephen J ; Cook‐Andersen, Heidi ; Wilkinson, Miles F</creatorcontrib><description>Testis‐expressed X‐linked genes typically evolve rapidly. Here, we report on a testis‐expressed X‐linked microRNA (miRNA) cluster that despite rapid alterations in sequence has retained its position in the Fragile‐X region of the X chromosome in placental mammals. Surprisingly, the miRNAs encoded by this cluster (
Fx‐mir
) have a predilection for targeting the immediately adjacent gene,
Fmr1
, an unexpected finding given that miRNAs usually act
in trans
, not
in cis
. Robust repression of
Fmr1
is conferred by combinations of
Fx‐mir
miRNAs induced in Sertoli cells (SCs) during postnatal development when they terminate proliferation. Physiological significance is suggested by the finding that FMRP, the protein product of
Fmr1
, is downregulated when
Fx‐mir
miRNAs are induced, and that FMRP loss causes SC hyperproliferation and spermatogenic defects.
Fx‐mir
miRNAs not only regulate the expression of FMRP, but also regulate the expression of eIF4E and CYFIP1, which together with FMRP form a translational regulatory complex. Our results support a model in which
Fx‐mir
family members act cooperatively to regulate the translation of batteries of mRNAs in a developmentally regulated manner in SCs.
Synopsis
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.
A large X‐linked miRNA cluster (
Fx‐mir
) is functionally characterized.
Members of
Fx‐mir
have a predilection for targeting the immediately adjacent gene
Fmr1
.
Fx‐mir
miRNAs are postnatally induced in Sertoli cells and act additively to repress
Fmr1
.
The
Fx‐mir
cluster is rapidly evolving in sequence but its ability to target
Fmr1
is conserved.
Graphical Abstract
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.</description><identifier>ISSN: 1469-221X</identifier><identifier>EISSN: 1469-3178</identifier><identifier>DOI: 10.15252/embr.201846566</identifier><identifier>PMID: 30573526</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>3' Untranslated Regions ; Animals ; Biological evolution ; Cell proliferation ; Clusters ; Conserved sequence ; EMBO11 ; EMBO24 ; EMBO36 ; evolution ; FMR1 ; Fragile X Mental Retardation Protein - genetics ; Gene Expression Regulation ; Humans ; Initiation factor eIF-4E ; Male ; Mice ; microRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Multigene Family ; Proteins ; Ribonucleic acid ; RNA ; RNA Interference ; RNA, Messenger - genetics ; Sertoli cells ; Spermatogenesis ; Spermatogenesis - genetics ; testis ; Testis - metabolism ; translation ; X chromosomes</subject><ispartof>EMBO reports, 2019-02, Vol.20 (2), p.n/a</ispartof><rights>The Author(s) 2018</rights><rights>2018 The Authors. Published under the terms of the CC BY NC ND 4.0 license</rights><rights>2018 The Authors. Published under the terms of the CC BY NC ND 4.0 license.</rights><rights>2019 EMBO</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-6416-3058 ; 0000-0002-8567-7795</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362356/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362356/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27903,27904,41099,42168,45553,45554,46387,46811,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30573526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramaiah, Madhuvanthi</creatorcontrib><creatorcontrib>Tan, Kun</creatorcontrib><creatorcontrib>Plank, Terra‐Dawn M</creatorcontrib><creatorcontrib>Song, Hye‐Won</creatorcontrib><creatorcontrib>Chousal, Jennifer N</creatorcontrib><creatorcontrib>Jones, Samantha</creatorcontrib><creatorcontrib>Shum, Eleen Y</creatorcontrib><creatorcontrib>Sheridan, Steven D</creatorcontrib><creatorcontrib>Peterson, Kevin J</creatorcontrib><creatorcontrib>Gromoll, Jörg</creatorcontrib><creatorcontrib>Haggarty, Stephen J</creatorcontrib><creatorcontrib>Cook‐Andersen, Heidi</creatorcontrib><creatorcontrib>Wilkinson, Miles F</creatorcontrib><title>A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1</title><title>EMBO reports</title><addtitle>EMBO Rep</addtitle><addtitle>EMBO Rep</addtitle><description>Testis‐expressed X‐linked genes typically evolve rapidly. Here, we report on a testis‐expressed X‐linked microRNA (miRNA) cluster that despite rapid alterations in sequence has retained its position in the Fragile‐X region of the X chromosome in placental mammals. Surprisingly, the miRNAs encoded by this cluster (
Fx‐mir
) have a predilection for targeting the immediately adjacent gene,
Fmr1
, an unexpected finding given that miRNAs usually act
in trans
, not
in cis
. Robust repression of
Fmr1
is conferred by combinations of
Fx‐mir
miRNAs induced in Sertoli cells (SCs) during postnatal development when they terminate proliferation. Physiological significance is suggested by the finding that FMRP, the protein product of
Fmr1
, is downregulated when
Fx‐mir
miRNAs are induced, and that FMRP loss causes SC hyperproliferation and spermatogenic defects.
Fx‐mir
miRNAs not only regulate the expression of FMRP, but also regulate the expression of eIF4E and CYFIP1, which together with FMRP form a translational regulatory complex. Our results support a model in which
Fx‐mir
family members act cooperatively to regulate the translation of batteries of mRNAs in a developmentally regulated manner in SCs.
Synopsis
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.
A large X‐linked miRNA cluster (
Fx‐mir
) is functionally characterized.
Members of
Fx‐mir
have a predilection for targeting the immediately adjacent gene
Fmr1
.
Fx‐mir
miRNAs are postnatally induced in Sertoli cells and act additively to repress
Fmr1
.
The
Fx‐mir
cluster is rapidly evolving in sequence but its ability to target
Fmr1
is conserved.
Graphical Abstract
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.</description><subject>3' Untranslated Regions</subject><subject>Animals</subject><subject>Biological evolution</subject><subject>Cell proliferation</subject><subject>Clusters</subject><subject>Conserved sequence</subject><subject>EMBO11</subject><subject>EMBO24</subject><subject>EMBO36</subject><subject>evolution</subject><subject>FMR1</subject><subject>Fragile X Mental Retardation Protein - genetics</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Initiation factor eIF-4E</subject><subject>Male</subject><subject>Mice</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Multigene Family</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>Sertoli cells</subject><subject>Spermatogenesis</subject><subject>Spermatogenesis - genetics</subject><subject>testis</subject><subject>Testis - metabolism</subject><subject>translation</subject><subject>X chromosomes</subject><issn>1469-221X</issn><issn>1469-3178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp1kU9PFDEYxhuCAVw9cyNNuHhZ7J9pO-VgshBWTRZNNpoQLk1n-s5QMv9oZxRufAQ_o5_EWXdd0MRT27zP-8vT50HokJITKphgb6HOwgkjNE2kkHIHHdBE6imnKt3d3BmjV_voZYy3hBChVbqH9jkRigsmD9D1DNc-D-3y0wzn1RB7CNg3uL8BPA-29BX8fPxxhQOUvm0w3HcBYgSH3RB8U-LYQaht35bQQPQR9zaU0Ec8v1zSV-hFYasIrzfnBH2dX3w5_zBdfH7_8Xy2mHY8lXKaOJI5TriiTBcSXKalo7lOmEgdEwpkkpOC2yRTnOpMMc4g5wVPOahEuCLjE_Ruze2GrAaXQ9MHW5ku-NqGB9Nab_6eNP7GlO03I7lkXMgR8GYDCO3dALE3tY85VJVtoB2iYVRoncoxvFF6_I_0th1CM35vVCmhtFBsBTx67mhr5U_so-B0Lfg-BvywnVNifrdqVq2abavm4vJsuX2Ny2S9HLtVBxCePPwHwH8BorenGg</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Ramaiah, Madhuvanthi</creator><creator>Tan, Kun</creator><creator>Plank, Terra‐Dawn M</creator><creator>Song, Hye‐Won</creator><creator>Chousal, Jennifer N</creator><creator>Jones, Samantha</creator><creator>Shum, Eleen Y</creator><creator>Sheridan, Steven D</creator><creator>Peterson, Kevin J</creator><creator>Gromoll, Jörg</creator><creator>Haggarty, Stephen J</creator><creator>Cook‐Andersen, Heidi</creator><creator>Wilkinson, Miles F</creator><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>John Wiley and Sons Inc</general><scope>C6C</scope><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6416-3058</orcidid><orcidid>https://orcid.org/0000-0002-8567-7795</orcidid></search><sort><creationdate>201902</creationdate><title>A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1</title><author>Ramaiah, Madhuvanthi ; Tan, Kun ; Plank, Terra‐Dawn M ; Song, Hye‐Won ; Chousal, Jennifer N ; Jones, Samantha ; Shum, Eleen Y ; Sheridan, Steven D ; Peterson, Kevin J ; Gromoll, Jörg ; Haggarty, Stephen J ; Cook‐Andersen, Heidi ; Wilkinson, Miles F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p3866-4d0bd3037129f6edb96d1c94258d257e64c0f3a4b7319b7232ec3f383e745dfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3' Untranslated Regions</topic><topic>Animals</topic><topic>Biological evolution</topic><topic>Cell proliferation</topic><topic>Clusters</topic><topic>Conserved sequence</topic><topic>EMBO11</topic><topic>EMBO24</topic><topic>EMBO36</topic><topic>evolution</topic><topic>FMR1</topic><topic>Fragile X Mental Retardation Protein - genetics</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Initiation factor eIF-4E</topic><topic>Male</topic><topic>Mice</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Multigene Family</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>Sertoli cells</topic><topic>Spermatogenesis</topic><topic>Spermatogenesis - genetics</topic><topic>testis</topic><topic>Testis - metabolism</topic><topic>translation</topic><topic>X chromosomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramaiah, Madhuvanthi</creatorcontrib><creatorcontrib>Tan, Kun</creatorcontrib><creatorcontrib>Plank, Terra‐Dawn M</creatorcontrib><creatorcontrib>Song, Hye‐Won</creatorcontrib><creatorcontrib>Chousal, Jennifer N</creatorcontrib><creatorcontrib>Jones, Samantha</creatorcontrib><creatorcontrib>Shum, Eleen Y</creatorcontrib><creatorcontrib>Sheridan, Steven D</creatorcontrib><creatorcontrib>Peterson, Kevin J</creatorcontrib><creatorcontrib>Gromoll, Jörg</creatorcontrib><creatorcontrib>Haggarty, Stephen J</creatorcontrib><creatorcontrib>Cook‐Andersen, Heidi</creatorcontrib><creatorcontrib>Wilkinson, Miles F</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>EMBO reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramaiah, Madhuvanthi</au><au>Tan, Kun</au><au>Plank, Terra‐Dawn M</au><au>Song, Hye‐Won</au><au>Chousal, Jennifer N</au><au>Jones, Samantha</au><au>Shum, Eleen Y</au><au>Sheridan, Steven D</au><au>Peterson, Kevin J</au><au>Gromoll, Jörg</au><au>Haggarty, Stephen J</au><au>Cook‐Andersen, Heidi</au><au>Wilkinson, Miles F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1</atitle><jtitle>EMBO reports</jtitle><stitle>EMBO Rep</stitle><addtitle>EMBO Rep</addtitle><date>2019-02</date><risdate>2019</risdate><volume>20</volume><issue>2</issue><epage>n/a</epage><issn>1469-221X</issn><eissn>1469-3178</eissn><abstract>Testis‐expressed X‐linked genes typically evolve rapidly. Here, we report on a testis‐expressed X‐linked microRNA (miRNA) cluster that despite rapid alterations in sequence has retained its position in the Fragile‐X region of the X chromosome in placental mammals. Surprisingly, the miRNAs encoded by this cluster (
Fx‐mir
) have a predilection for targeting the immediately adjacent gene,
Fmr1
, an unexpected finding given that miRNAs usually act
in trans
, not
in cis
. Robust repression of
Fmr1
is conferred by combinations of
Fx‐mir
miRNAs induced in Sertoli cells (SCs) during postnatal development when they terminate proliferation. Physiological significance is suggested by the finding that FMRP, the protein product of
Fmr1
, is downregulated when
Fx‐mir
miRNAs are induced, and that FMRP loss causes SC hyperproliferation and spermatogenic defects.
Fx‐mir
miRNAs not only regulate the expression of FMRP, but also regulate the expression of eIF4E and CYFIP1, which together with FMRP form a translational regulatory complex. Our results support a model in which
Fx‐mir
family members act cooperatively to regulate the translation of batteries of mRNAs in a developmentally regulated manner in SCs.
Synopsis
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.
A large X‐linked miRNA cluster (
Fx‐mir
) is functionally characterized.
Members of
Fx‐mir
have a predilection for targeting the immediately adjacent gene
Fmr1
.
Fx‐mir
miRNAs are postnatally induced in Sertoli cells and act additively to repress
Fmr1
.
The
Fx‐mir
cluster is rapidly evolving in sequence but its ability to target
Fmr1
is conserved.
Graphical Abstract
The rapidly evolving X‐linked microRNA cluster
Fx‐mir
is strongly induced during Sertoli cell development.
Fx‐mir
miRNAs target components of a translational regulatory complex, including the Fragile‐X protein FMRP, the initiation factor eIF4E and the repressor CYFIP1.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30573526</pmid><doi>10.15252/embr.201846566</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-6416-3058</orcidid><orcidid>https://orcid.org/0000-0002-8567-7795</orcidid><oa>free_for_read</oa></addata></record> |
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source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Springer Nature OA Free Journals |
subjects | 3' Untranslated Regions Animals Biological evolution Cell proliferation Clusters Conserved sequence EMBO11 EMBO24 EMBO36 evolution FMR1 Fragile X Mental Retardation Protein - genetics Gene Expression Regulation Humans Initiation factor eIF-4E Male Mice microRNA MicroRNAs MicroRNAs - genetics miRNA Multigene Family Proteins Ribonucleic acid RNA RNA Interference RNA, Messenger - genetics Sertoli cells Spermatogenesis Spermatogenesis - genetics testis Testis - metabolism translation X chromosomes |
title | A microRNA cluster in the Fragile‐X region expressed during spermatogenesis targets FMR1 |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T06%3A33%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20microRNA%20cluster%20in%20the%20Fragile%E2%80%90X%20region%20expressed%20during%20spermatogenesis%20targets%20FMR1&rft.jtitle=EMBO%20reports&rft.au=Ramaiah,%20Madhuvanthi&rft.date=2019-02&rft.volume=20&rft.issue=2&rft.epage=n/a&rft.issn=1469-221X&rft.eissn=1469-3178&rft_id=info:doi/10.15252/embr.201846566&rft_dat=%3Cproquest_pubme%3E2159986059%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2175795726&rft_id=info:pmid/30573526&rfr_iscdi=true |