Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR

A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5’-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2019, Vol.81(1), pp.103-106
Hauptverfasser:	GOTO, Yusuke, YAEGASHI, Gakuji, KUMAGAI, Yoshihiro, OGASAWARA, Fusae, GOTO, Makiko, MASE, Masaji
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals avian encephalomyelitis virus Avian Pathology Chickens Encephalomyelitis Encephalomyelitis Virus, Avian - genetics Encephalomyelitis Virus, Avian - isolation & purification identification Japan - epidemiology Phylogeny Picornaviridae Infections - diagnosis Picornaviridae Infections - epidemiology Picornaviridae Infections - veterinary Picornaviridae Infections - virology Polymerase chain reaction Poultry Poultry Diseases - diagnosis Poultry Diseases - epidemiology Poultry Diseases - virology Primers Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription RT-PCR Viruses
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