Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation
Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We applied the probe of 99mTc-scFv-VCAM-1 to...
Gespeichert in:
| Veröffentlicht in: | Contrast media and molecular imaging 2019-01, Vol.2019 (2019), p.1-8 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8 |
|---|---|
| container_issue | 2019 |
| container_start_page | 1 |
| container_title | Contrast media and molecular imaging |
| container_volume | 2019 |
| creator | Lu, Diyu Zhang, Yongxue Xu, Shufang Zhou, Junfen Xia, Liang Cheng, Xiaojie Zhou, Jun Liu, Chunbao Wang, Yichun |
| description | Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We applied the probe of 99mTc-scFv-VCAM-1 to colitis rabbit to examine its imaging performance. The colitis model rabbit was prepared, and a typical inflammatory lesion was confirmed in the colon. The probe of 99mTc-scFv-VCAM-1 was synthesized and injected into the model animal before imaging examination. Scintigraphy detected colitis lesions in both SPECT planar and SPECT/CT fused images, with higher target-to-nontarget ratios in the model group (2.71 ± 0.31) than those in the control group (1.12 ± 0.10). Biodistribution study determined tracer uptake in different organs, and autoradiography (ARG) confirmed probe accumulation in colon lesions. The uptake ratio of the model colon to the control colon was 4.71 ± 0.61 in quantitative analysis of the ARG regions of interest. Stronger VCAM-1 expression in the model colon than that in the control colon was confirmed by western blotting and immunohistochemistry. Our imaging study indicates molecular imaging with scFv-VCAM-1 as a promising way for inflammatory bowel disease diagnosis and evaluation. |
| doi_str_mv | 10.1155/2019/2783519 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6360587</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2186143385</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-9c6c56110fb60c299a59e01a84468efe60f6da8e7cfbc688b99e6f736e2cfcf73</originalsourceid><addsrcrecordid>eNqNkUtv1DAUhSMEog_YsUaW2CCVUDtOHHuDNBpRGKkIxGtrOc51xpUnbu2kqP-Cn8wdzXR4rFj5-t7PR_f4FMUzRl8z1jTnFWXqvGolb5h6UBxjqylrztqHh5qqo-Ik5ytK65or_rg44lTSuq34cfHzix-HAOVybfxIvpvkTReAXCQzbGCcyGKcfBf7OxIdTrOdg0lkCSGQRb-G7ONIPsQA2AfCiMnE3N-RW23MgPLkU4odEBfxZQx-8hmZHgL5bLrOT2Q13kKe_GAmlHtSPHImZHi6P0-Lbxdvvy7fl5cf362Wi8vS1i2bSmWFbQRj1HWC2kop0yigzMi6FhIcCOpEbyS01nVWSNkpBcK1XEBlncXitHiz072euw30Fs0mE_R18huT7nQ0Xv89Gf1aD_FWCy5oI7cCL_cCKd7MaEBvfLb4M2aEOGddMSlYzblsEH3xD3oV5zSiPaQUZaySjULq1Y6yKeacwB2WYVRvo9bbqPU-asSf_2ngAN9ni8DZDlj7sTc__H_KATLgzG-aceQY_wXFsbxn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2190112859</pqid></control><display><type>article</type><title>Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Lu, Diyu ; Zhang, Yongxue ; Xu, Shufang ; Zhou, Junfen ; Xia, Liang ; Cheng, Xiaojie ; Zhou, Jun ; Liu, Chunbao ; Wang, Yichun</creator><contributor>Malviya, Gaurav ; Gaurav Malviya</contributor><creatorcontrib>Lu, Diyu ; Zhang, Yongxue ; Xu, Shufang ; Zhou, Junfen ; Xia, Liang ; Cheng, Xiaojie ; Zhou, Jun ; Liu, Chunbao ; Wang, Yichun ; Malviya, Gaurav ; Gaurav Malviya</creatorcontrib><description>Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We applied the probe of 99mTc-scFv-VCAM-1 to colitis rabbit to examine its imaging performance. The colitis model rabbit was prepared, and a typical inflammatory lesion was confirmed in the colon. The probe of 99mTc-scFv-VCAM-1 was synthesized and injected into the model animal before imaging examination. Scintigraphy detected colitis lesions in both SPECT planar and SPECT/CT fused images, with higher target-to-nontarget ratios in the model group (2.71 ± 0.31) than those in the control group (1.12 ± 0.10). Biodistribution study determined tracer uptake in different organs, and autoradiography (ARG) confirmed probe accumulation in colon lesions. The uptake ratio of the model colon to the control colon was 4.71 ± 0.61 in quantitative analysis of the ARG regions of interest. Stronger VCAM-1 expression in the model colon than that in the control colon was confirmed by western blotting and immunohistochemistry. Our imaging study indicates molecular imaging with scFv-VCAM-1 as a promising way for inflammatory bowel disease diagnosis and evaluation.</description><identifier>ISSN: 1555-4309</identifier><identifier>EISSN: 1555-4317</identifier><identifier>DOI: 10.1155/2019/2783519</identifier><identifier>PMID: 30804723</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Adhesion ; Animals ; Atherosclerosis ; Autoradiography ; Blotting, Western ; Catheters ; Cell adhesion ; Cell adhesion & migration ; Cell adhesion molecules ; Chains ; Colitis ; Colitis - diagnostic imaging ; Colitis - immunology ; Colitis - metabolism ; Colon ; Computed tomography ; Drug dosages ; Endoscopy ; Image detection ; Immunohistochemistry ; Inflammation ; Inflammatory bowel disease ; Inflammatory bowel diseases ; Intestine ; Lesions ; Male ; Medical imaging ; Molecular Probes ; Organs ; Pathogenesis ; Physiology ; Quantitative analysis ; Quantum dots ; Rabbits ; Scintigraphy ; Single photon emission computed tomography ; Single-Chain Antibodies ; Sodium ; Target recognition ; Tomography, Emission-Computed, Single-Photon ; Ultrasonic imaging ; Vascular cell adhesion molecule 1 ; Vascular Cell Adhesion Molecule-1 - immunology ; Vascular Cell Adhesion Molecule-1 - metabolism ; Western blotting</subject><ispartof>Contrast media and molecular imaging, 2019-01, Vol.2019 (2019), p.1-8</ispartof><rights>Copyright © 2019 Chunbao Liu et al.</rights><rights>Copyright © 2019 Chunbao Liu et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Chunbao Liu et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-9c6c56110fb60c299a59e01a84468efe60f6da8e7cfbc688b99e6f736e2cfcf73</citedby><cites>FETCH-LOGICAL-c471t-9c6c56110fb60c299a59e01a84468efe60f6da8e7cfbc688b99e6f736e2cfcf73</cites><orcidid>0000-0003-2532-7133 ; 0000-0003-4600-9476 ; 0000-0001-9172-7878</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360587/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360587/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30804723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Malviya, Gaurav</contributor><contributor>Gaurav Malviya</contributor><creatorcontrib>Lu, Diyu</creatorcontrib><creatorcontrib>Zhang, Yongxue</creatorcontrib><creatorcontrib>Xu, Shufang</creatorcontrib><creatorcontrib>Zhou, Junfen</creatorcontrib><creatorcontrib>Xia, Liang</creatorcontrib><creatorcontrib>Cheng, Xiaojie</creatorcontrib><creatorcontrib>Zhou, Jun</creatorcontrib><creatorcontrib>Liu, Chunbao</creatorcontrib><creatorcontrib>Wang, Yichun</creatorcontrib><title>Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation</title><title>Contrast media and molecular imaging</title><addtitle>Contrast Media Mol Imaging</addtitle><description>Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We applied the probe of 99mTc-scFv-VCAM-1 to colitis rabbit to examine its imaging performance. The colitis model rabbit was prepared, and a typical inflammatory lesion was confirmed in the colon. The probe of 99mTc-scFv-VCAM-1 was synthesized and injected into the model animal before imaging examination. Scintigraphy detected colitis lesions in both SPECT planar and SPECT/CT fused images, with higher target-to-nontarget ratios in the model group (2.71 ± 0.31) than those in the control group (1.12 ± 0.10). Biodistribution study determined tracer uptake in different organs, and autoradiography (ARG) confirmed probe accumulation in colon lesions. The uptake ratio of the model colon to the control colon was 4.71 ± 0.61 in quantitative analysis of the ARG regions of interest. Stronger VCAM-1 expression in the model colon than that in the control colon was confirmed by western blotting and immunohistochemistry. Our imaging study indicates molecular imaging with scFv-VCAM-1 as a promising way for inflammatory bowel disease diagnosis and evaluation.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Atherosclerosis</subject><subject>Autoradiography</subject><subject>Blotting, Western</subject><subject>Catheters</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell adhesion molecules</subject><subject>Chains</subject><subject>Colitis</subject><subject>Colitis - diagnostic imaging</subject><subject>Colitis - immunology</subject><subject>Colitis - metabolism</subject><subject>Colon</subject><subject>Computed tomography</subject><subject>Drug dosages</subject><subject>Endoscopy</subject><subject>Image detection</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>Inflammatory bowel diseases</subject><subject>Intestine</subject><subject>Lesions</subject><subject>Male</subject><subject>Medical imaging</subject><subject>Molecular Probes</subject><subject>Organs</subject><subject>Pathogenesis</subject><subject>Physiology</subject><subject>Quantitative analysis</subject><subject>Quantum dots</subject><subject>Rabbits</subject><subject>Scintigraphy</subject><subject>Single photon emission computed tomography</subject><subject>Single-Chain Antibodies</subject><subject>Sodium</subject><subject>Target recognition</subject><subject>Tomography, Emission-Computed, Single-Photon</subject><subject>Ultrasonic imaging</subject><subject>Vascular cell adhesion molecule 1</subject><subject>Vascular Cell Adhesion Molecule-1 - immunology</subject><subject>Vascular Cell Adhesion Molecule-1 - metabolism</subject><subject>Western blotting</subject><issn>1555-4309</issn><issn>1555-4317</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkUtv1DAUhSMEog_YsUaW2CCVUDtOHHuDNBpRGKkIxGtrOc51xpUnbu2kqP-Cn8wdzXR4rFj5-t7PR_f4FMUzRl8z1jTnFWXqvGolb5h6UBxjqylrztqHh5qqo-Ik5ytK65or_rg44lTSuq34cfHzix-HAOVybfxIvpvkTReAXCQzbGCcyGKcfBf7OxIdTrOdg0lkCSGQRb-G7ONIPsQA2AfCiMnE3N-RW23MgPLkU4odEBfxZQx-8hmZHgL5bLrOT2Q13kKe_GAmlHtSPHImZHi6P0-Lbxdvvy7fl5cf362Wi8vS1i2bSmWFbQRj1HWC2kop0yigzMi6FhIcCOpEbyS01nVWSNkpBcK1XEBlncXitHiz072euw30Fs0mE_R18huT7nQ0Xv89Gf1aD_FWCy5oI7cCL_cCKd7MaEBvfLb4M2aEOGddMSlYzblsEH3xD3oV5zSiPaQUZaySjULq1Y6yKeacwB2WYVRvo9bbqPU-asSf_2ngAN9ni8DZDlj7sTc__H_KATLgzG-aceQY_wXFsbxn</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Lu, Diyu</creator><creator>Zhang, Yongxue</creator><creator>Xu, Shufang</creator><creator>Zhou, Junfen</creator><creator>Xia, Liang</creator><creator>Cheng, Xiaojie</creator><creator>Zhou, Jun</creator><creator>Liu, Chunbao</creator><creator>Wang, Yichun</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2532-7133</orcidid><orcidid>https://orcid.org/0000-0003-4600-9476</orcidid><orcidid>https://orcid.org/0000-0001-9172-7878</orcidid></search><sort><creationdate>20190101</creationdate><title>Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation</title><author>Lu, Diyu ; Zhang, Yongxue ; Xu, Shufang ; Zhou, Junfen ; Xia, Liang ; Cheng, Xiaojie ; Zhou, Jun ; Liu, Chunbao ; Wang, Yichun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-9c6c56110fb60c299a59e01a84468efe60f6da8e7cfbc688b99e6f736e2cfcf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Atherosclerosis</topic><topic>Autoradiography</topic><topic>Blotting, Western</topic><topic>Catheters</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell adhesion molecules</topic><topic>Chains</topic><topic>Colitis</topic><topic>Colitis - diagnostic imaging</topic><topic>Colitis - immunology</topic><topic>Colitis - metabolism</topic><topic>Colon</topic><topic>Computed tomography</topic><topic>Drug dosages</topic><topic>Endoscopy</topic><topic>Image detection</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Inflammatory bowel disease</topic><topic>Inflammatory bowel diseases</topic><topic>Intestine</topic><topic>Lesions</topic><topic>Male</topic><topic>Medical imaging</topic><topic>Molecular Probes</topic><topic>Organs</topic><topic>Pathogenesis</topic><topic>Physiology</topic><topic>Quantitative analysis</topic><topic>Quantum dots</topic><topic>Rabbits</topic><topic>Scintigraphy</topic><topic>Single photon emission computed tomography</topic><topic>Single-Chain Antibodies</topic><topic>Sodium</topic><topic>Target recognition</topic><topic>Tomography, Emission-Computed, Single-Photon</topic><topic>Ultrasonic imaging</topic><topic>Vascular cell adhesion molecule 1</topic><topic>Vascular Cell Adhesion Molecule-1 - immunology</topic><topic>Vascular Cell Adhesion Molecule-1 - metabolism</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Diyu</creatorcontrib><creatorcontrib>Zhang, Yongxue</creatorcontrib><creatorcontrib>Xu, Shufang</creatorcontrib><creatorcontrib>Zhou, Junfen</creatorcontrib><creatorcontrib>Xia, Liang</creatorcontrib><creatorcontrib>Cheng, Xiaojie</creatorcontrib><creatorcontrib>Zhou, Jun</creatorcontrib><creatorcontrib>Liu, Chunbao</creatorcontrib><creatorcontrib>Wang, Yichun</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Contrast media and molecular imaging</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Diyu</au><au>Zhang, Yongxue</au><au>Xu, Shufang</au><au>Zhou, Junfen</au><au>Xia, Liang</au><au>Cheng, Xiaojie</au><au>Zhou, Jun</au><au>Liu, Chunbao</au><au>Wang, Yichun</au><au>Malviya, Gaurav</au><au>Gaurav Malviya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation</atitle><jtitle>Contrast media and molecular imaging</jtitle><addtitle>Contrast Media Mol Imaging</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1555-4309</issn><eissn>1555-4317</eissn><abstract>Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We applied the probe of 99mTc-scFv-VCAM-1 to colitis rabbit to examine its imaging performance. The colitis model rabbit was prepared, and a typical inflammatory lesion was confirmed in the colon. The probe of 99mTc-scFv-VCAM-1 was synthesized and injected into the model animal before imaging examination. Scintigraphy detected colitis lesions in both SPECT planar and SPECT/CT fused images, with higher target-to-nontarget ratios in the model group (2.71 ± 0.31) than those in the control group (1.12 ± 0.10). Biodistribution study determined tracer uptake in different organs, and autoradiography (ARG) confirmed probe accumulation in colon lesions. The uptake ratio of the model colon to the control colon was 4.71 ± 0.61 in quantitative analysis of the ARG regions of interest. Stronger VCAM-1 expression in the model colon than that in the control colon was confirmed by western blotting and immunohistochemistry. Our imaging study indicates molecular imaging with scFv-VCAM-1 as a promising way for inflammatory bowel disease diagnosis and evaluation.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>30804723</pmid><doi>10.1155/2019/2783519</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2532-7133</orcidid><orcidid>https://orcid.org/0000-0003-4600-9476</orcidid><orcidid>https://orcid.org/0000-0001-9172-7878</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1555-4309 |
| ispartof | Contrast media and molecular imaging, 2019-01, Vol.2019 (2019), p.1-8 |
| issn | 1555-4309 1555-4317 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6360587 |
| source | MEDLINE; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adhesion Animals Atherosclerosis Autoradiography Blotting, Western Catheters Cell adhesion Cell adhesion & migration Cell adhesion molecules Chains Colitis Colitis - diagnostic imaging Colitis - immunology Colitis - metabolism Colon Computed tomography Drug dosages Endoscopy Image detection Immunohistochemistry Inflammation Inflammatory bowel disease Inflammatory bowel diseases Intestine Lesions Male Medical imaging Molecular Probes Organs Pathogenesis Physiology Quantitative analysis Quantum dots Rabbits Scintigraphy Single photon emission computed tomography Single-Chain Antibodies Sodium Target recognition Tomography, Emission-Computed, Single-Photon Ultrasonic imaging Vascular cell adhesion molecule 1 Vascular Cell Adhesion Molecule-1 - immunology Vascular Cell Adhesion Molecule-1 - metabolism Western blotting |
| title | Single-Chain Variable Fragment Antibody of Vascular Cell Adhesion Molecule 1 as a Molecular Imaging Probe for Colitis Model Rabbit Investigation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T09%3A13%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single-Chain%20Variable%20Fragment%20Antibody%20of%20Vascular%20Cell%20Adhesion%20Molecule%201%20as%20a%20Molecular%20Imaging%20Probe%20for%20Colitis%20Model%20Rabbit%20Investigation&rft.jtitle=Contrast%20media%20and%20molecular%20imaging&rft.au=Lu,%20Diyu&rft.date=2019-01-01&rft.volume=2019&rft.issue=2019&rft.spage=1&rft.epage=8&rft.pages=1-8&rft.issn=1555-4309&rft.eissn=1555-4317&rft_id=info:doi/10.1155/2019/2783519&rft_dat=%3Cproquest_pubme%3E2186143385%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2190112859&rft_id=info:pmid/30804723&rfr_iscdi=true |