Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells
In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris. In this study, we used bioassay guided fractionation to identify compounds from G. acuta a...
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| creator | Li, Min-Hui Dang, Lian-sheng Gong, Xue Yi, Le-tai Lv, Li-juan Su, He Ren, Kai Zhang, Rui-fen |
| description | In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris. In this study, we used bioassay guided fractionation to identify compounds from G. acuta and investigated their activity against hydrogen peroxide (H2O2)-induced apoptosis of H9c2 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic (GCLC) expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was evaluated using western blot. The results showed that all four compounds had protective effects on H9c2 cells. The transcription levels of HO-1 and GCLC significantly increased in H9c2 cells pretreated with norswertianolin (1), swetrianolin (2), demethylbellidifolin (3), and bellidifolin (4). However, compared to the model group, the transcription levels of Nrf2 were not enhanced by pretreatment with compounds 1, 2, and 4. The protein expression levels of HO-1 and GCLC in H9c2 cells were greater than that in the H2O2-treated group, and the expression of Nrf2 was not significantly changed except by swetrianolin treatment; inhibitors can reverse the protective effect by ZnPP (15 μM), BSO (10 μM), and brusatol (10 μM). The results indicated that the four compounds isolated from G. acuta inhibited the oxidative injury induced by H2O2 by activating the Nrf2/ARE pathway in H9c2 cells and provide evidence that G. acuta may be a potential therapeutic agent for the treatment of cardiovascular diseases. |
| doi_str_mv | 10.1155/2019/2692970 |
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Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells</title><source>PubMed Central Open Access</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Li, Min-Hui ; Dang, Lian-sheng ; Gong, Xue ; Yi, Le-tai ; Lv, Li-juan ; Su, He ; Ren, Kai ; Zhang, Rui-fen</creator><contributor>Pabelick, Christina ; Christina Pabelick</contributor><creatorcontrib>Li, Min-Hui ; Dang, Lian-sheng ; Gong, Xue ; Yi, Le-tai ; Lv, Li-juan ; Su, He ; Ren, Kai ; Zhang, Rui-fen ; Pabelick, Christina ; Christina Pabelick</creatorcontrib><description>In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris. In this study, we used bioassay guided fractionation to identify compounds from G. acuta and investigated their activity against hydrogen peroxide (H2O2)-induced apoptosis of H9c2 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic (GCLC) expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was evaluated using western blot. The results showed that all four compounds had protective effects on H9c2 cells. The transcription levels of HO-1 and GCLC significantly increased in H9c2 cells pretreated with norswertianolin (1), swetrianolin (2), demethylbellidifolin (3), and bellidifolin (4). However, compared to the model group, the transcription levels of Nrf2 were not enhanced by pretreatment with compounds 1, 2, and 4. The protein expression levels of HO-1 and GCLC in H9c2 cells were greater than that in the H2O2-treated group, and the expression of Nrf2 was not significantly changed except by swetrianolin treatment; inhibitors can reverse the protective effect by ZnPP (15 μM), BSO (10 μM), and brusatol (10 μM). The results indicated that the four compounds isolated from G. acuta inhibited the oxidative injury induced by H2O2 by activating the Nrf2/ARE pathway in H9c2 cells and provide evidence that G. acuta may be a potential therapeutic agent for the treatment of cardiovascular diseases.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2019/2692970</identifier><identifier>PMID: 30800665</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Angina ; Angina pectoris ; Antioxidants ; Apoptosis ; Bioassays ; Biotechnology industry ; Cardiomyocytes ; Cardiovascular disease ; Cardiovascular diseases ; Catalysis ; Chemical compounds ; Chromatography ; Fever ; Fractionation ; Gentianella ; Glutamate-cysteine ligase ; Headache ; Heart ; Heart diseases ; Heme ; Heme oxygenase (decyclizing) ; Hepatitis ; Hydrogen peroxide ; Ischemia ; Jaundice ; Medical treatment ; Medicine ; Oxidative stress ; Oxygenase ; Penicillin ; Pharmacology ; Polymerase chain reaction ; Proteins ; Sterols ; Terpenes ; Transcription</subject><ispartof>BioMed research international, 2019-01, Vol.2019 (2019), p.1-9</ispartof><rights>Copyright © 2019 Kai Ren et al.</rights><rights>Copyright © 2019 Kai Ren et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2019 Kai Ren et al. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-d39919c208ec1128585e82b86343b4a8de3fb9fce839340bc7a2d64dd9e7d4093</citedby><cites>FETCH-LOGICAL-c471t-d39919c208ec1128585e82b86343b4a8de3fb9fce839340bc7a2d64dd9e7d4093</cites><orcidid>0000-0002-5366-8464</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360564/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360564/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30800665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pabelick, Christina</contributor><contributor>Christina Pabelick</contributor><creatorcontrib>Li, Min-Hui</creatorcontrib><creatorcontrib>Dang, Lian-sheng</creatorcontrib><creatorcontrib>Gong, Xue</creatorcontrib><creatorcontrib>Yi, Le-tai</creatorcontrib><creatorcontrib>Lv, Li-juan</creatorcontrib><creatorcontrib>Su, He</creatorcontrib><creatorcontrib>Ren, Kai</creatorcontrib><creatorcontrib>Zhang, Rui-fen</creatorcontrib><title>Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris. In this study, we used bioassay guided fractionation to identify compounds from G. acuta and investigated their activity against hydrogen peroxide (H2O2)-induced apoptosis of H9c2 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic (GCLC) expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was evaluated using western blot. The results showed that all four compounds had protective effects on H9c2 cells. The transcription levels of HO-1 and GCLC significantly increased in H9c2 cells pretreated with norswertianolin (1), swetrianolin (2), demethylbellidifolin (3), and bellidifolin (4). However, compared to the model group, the transcription levels of Nrf2 were not enhanced by pretreatment with compounds 1, 2, and 4. The protein expression levels of HO-1 and GCLC in H9c2 cells were greater than that in the H2O2-treated group, and the expression of Nrf2 was not significantly changed except by swetrianolin treatment; inhibitors can reverse the protective effect by ZnPP (15 μM), BSO (10 μM), and brusatol (10 μM). The results indicated that the four compounds isolated from G. acuta inhibited the oxidative injury induced by H2O2 by activating the Nrf2/ARE pathway in H9c2 cells and provide evidence that G. acuta may be a potential therapeutic agent for the treatment of cardiovascular diseases.</description><subject>Angina</subject><subject>Angina pectoris</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Bioassays</subject><subject>Biotechnology industry</subject><subject>Cardiomyocytes</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Catalysis</subject><subject>Chemical compounds</subject><subject>Chromatography</subject><subject>Fever</subject><subject>Fractionation</subject><subject>Gentianella</subject><subject>Glutamate-cysteine ligase</subject><subject>Headache</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Heme</subject><subject>Heme oxygenase (decyclizing)</subject><subject>Hepatitis</subject><subject>Hydrogen peroxide</subject><subject>Ischemia</subject><subject>Jaundice</subject><subject>Medical treatment</subject><subject>Medicine</subject><subject>Oxidative stress</subject><subject>Oxygenase</subject><subject>Penicillin</subject><subject>Pharmacology</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Sterols</subject><subject>Terpenes</subject><subject>Transcription</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc1vEzEQxVcIRKvSG2dkiUsRbOuv9doXJBS1TaQiOMDZ8tqzjaONHexdaP57HCVNgRNz8Uj-zdO8eVX1muBLQprmimKirqhQVLX4WXVKGeG1IJw8P_aMnVTnOa9wKUkEVuJldcKwxFiI5rRK130Pdswo9ugmTgnN4noTp-Ay6lNco1sIozcBhsEgY6fRoIvP3i4fLt-h-TSMEFAMaL51Kd6X_iuk-OAd1IvgJgsOLcJqSlvkC6MsRbOik19VL3ozZDg_vGfV95vrb7N5fffldjH7dFdb3pKxdkwpUoawBEsIlY1sQNJOCsZZx410wPpO9RYkU4zjzraGOsGdU9A6jhU7qz7udTdTtwZni5NkBr1Jfm3SVkfj9d8_wS_1ffypBRO4EbwIXBwEUvwxQR712me7O0WAOGVNSVmqZYqTgr79B12VY4Zir1BtQxklXBbqw56yKeacoD8uQ7De5al3eepDngV_86eBI_yYXgHe74GlD8788v8pB4WB3jzRhHJMCfsNooCwmQ</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Li, Min-Hui</creator><creator>Dang, Lian-sheng</creator><creator>Gong, Xue</creator><creator>Yi, Le-tai</creator><creator>Lv, Li-juan</creator><creator>Su, He</creator><creator>Ren, Kai</creator><creator>Zhang, Rui-fen</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5366-8464</orcidid></search><sort><creationdate>20190101</creationdate><title>Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells</title><author>Li, Min-Hui ; Dang, Lian-sheng ; Gong, Xue ; Yi, Le-tai ; Lv, Li-juan ; Su, He ; Ren, Kai ; Zhang, Rui-fen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-d39919c208ec1128585e82b86343b4a8de3fb9fce839340bc7a2d64dd9e7d4093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Angina</topic><topic>Angina pectoris</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Bioassays</topic><topic>Biotechnology industry</topic><topic>Cardiomyocytes</topic><topic>Cardiovascular disease</topic><topic>Cardiovascular diseases</topic><topic>Catalysis</topic><topic>Chemical compounds</topic><topic>Chromatography</topic><topic>Fever</topic><topic>Fractionation</topic><topic>Gentianella</topic><topic>Glutamate-cysteine ligase</topic><topic>Headache</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Heme</topic><topic>Heme oxygenase (decyclizing)</topic><topic>Hepatitis</topic><topic>Hydrogen peroxide</topic><topic>Ischemia</topic><topic>Jaundice</topic><topic>Medical treatment</topic><topic>Medicine</topic><topic>Oxidative stress</topic><topic>Oxygenase</topic><topic>Penicillin</topic><topic>Pharmacology</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Sterols</topic><topic>Terpenes</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Min-Hui</creatorcontrib><creatorcontrib>Dang, Lian-sheng</creatorcontrib><creatorcontrib>Gong, Xue</creatorcontrib><creatorcontrib>Yi, Le-tai</creatorcontrib><creatorcontrib>Lv, Li-juan</creatorcontrib><creatorcontrib>Su, He</creatorcontrib><creatorcontrib>Ren, Kai</creatorcontrib><creatorcontrib>Zhang, Rui-fen</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Min-Hui</au><au>Dang, Lian-sheng</au><au>Gong, Xue</au><au>Yi, Le-tai</au><au>Lv, Li-juan</au><au>Su, He</au><au>Ren, Kai</au><au>Zhang, Rui-fen</au><au>Pabelick, Christina</au><au>Christina Pabelick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>In previous studies, Gentianella acuta (Michx.) Hulten was reported to contain xanthones, iridoids, terpenoids, and sterols and is mainly used to cure hepatitis, jaundice, fever, headache, and angina pectoris. In this study, we used bioassay guided fractionation to identify compounds from G. acuta and investigated their activity against hydrogen peroxide (H2O2)-induced apoptosis of H9c2 cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic (GCLC) expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was evaluated using western blot. The results showed that all four compounds had protective effects on H9c2 cells. The transcription levels of HO-1 and GCLC significantly increased in H9c2 cells pretreated with norswertianolin (1), swetrianolin (2), demethylbellidifolin (3), and bellidifolin (4). However, compared to the model group, the transcription levels of Nrf2 were not enhanced by pretreatment with compounds 1, 2, and 4. The protein expression levels of HO-1 and GCLC in H9c2 cells were greater than that in the H2O2-treated group, and the expression of Nrf2 was not significantly changed except by swetrianolin treatment; inhibitors can reverse the protective effect by ZnPP (15 μM), BSO (10 μM), and brusatol (10 μM). The results indicated that the four compounds isolated from G. acuta inhibited the oxidative injury induced by H2O2 by activating the Nrf2/ARE pathway in H9c2 cells and provide evidence that G. acuta may be a potential therapeutic agent for the treatment of cardiovascular diseases.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>30800665</pmid><doi>10.1155/2019/2692970</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5366-8464</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angina Angina pectoris Antioxidants Apoptosis Bioassays Biotechnology industry Cardiomyocytes Cardiovascular disease Cardiovascular diseases Catalysis Chemical compounds Chromatography Fever Fractionation Gentianella Glutamate-cysteine ligase Headache Heart Heart diseases Heme Heme oxygenase (decyclizing) Hepatitis Hydrogen peroxide Ischemia Jaundice Medical treatment Medicine Oxidative stress Oxygenase Penicillin Pharmacology Polymerase chain reaction Proteins Sterols Terpenes Transcription |
| title | Effects of Four Compounds from Gentianella acuta (Michx.) Hulten on Hydrogen Peroxide-Induced Injury in H9c2 Cells |
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