Real-time tracking of fluorescent magnetic spore-based microrobots for remote detection of C. diff toxins
A rapid, direct, and low-cost method for detecting bacterial toxins associated with common gastrointestinal diseases remains a great challenge despite numerous studies and clinical assays. Motion-based detection through tracking the emerging micro- and nanorobots has shown great potential in chemo-...
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| Veröffentlicht in: | Science advances 2019-01, Vol.5 (1), p.eaau9650 |
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| creator | Zhang, Yabin Zhang, Lin Yang, Lidong Vong, Chi Ian Chan, Kai Fung Wu, William K K Kwong, Thomas N Y Lo, Norman W S Ip, Margaret Wong, Sunny H Sung, Joseph J Y Chiu, Philip W Y Zhang, Li |
| description | A rapid, direct, and low-cost method for detecting bacterial toxins associated with common gastrointestinal diseases remains a great challenge despite numerous studies and clinical assays. Motion-based detection through tracking the emerging micro- and nanorobots has shown great potential in chemo- and biosensing due to accelerated "chemistry on the move". Here, we described the use of fluorescent magnetic spore-based microrobots (FMSMs) as a highly efficient mobile sensing platform for the detection of toxins secreted by
(
) that were present in patients' stool. These microrobots were synthesized rapidly and inexpensively by the direct deposition of magnetic nanoparticles and the subsequent encapsulation of sensing probes on the porous natural spores. Because of the cooperation effect of natural spore, magnetic Fe
O
nanoparticles, and functionalized carbon nanodots, selective fluorescence detection of the prepared FMSMs is demonstrated in
bacterial supernatant and even in actual clinical stool samples from infectious patients within tens of minutes, suggesting rapid response and good selectivity and sensitivity of FMSMs toward
toxins. |
| doi_str_mv | 10.1126/sciadv.aau9650 |
| format | Article |
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(
) that were present in patients' stool. These microrobots were synthesized rapidly and inexpensively by the direct deposition of magnetic nanoparticles and the subsequent encapsulation of sensing probes on the porous natural spores. Because of the cooperation effect of natural spore, magnetic Fe
O
nanoparticles, and functionalized carbon nanodots, selective fluorescence detection of the prepared FMSMs is demonstrated in
bacterial supernatant and even in actual clinical stool samples from infectious patients within tens of minutes, suggesting rapid response and good selectivity and sensitivity of FMSMs toward
toxins.</description><identifier>ISSN: 2375-2548</identifier><identifier>EISSN: 2375-2548</identifier><identifier>DOI: 10.1126/sciadv.aau9650</identifier><identifier>PMID: 30746470</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Applied Sciences and Engineering ; Bacterial Proteins - analysis ; Bacterial Toxins - analysis ; Carbon - chemistry ; Clostridium difficile - physiology ; Clostridium Infections - diagnosis ; Clostridium Infections - microbiology ; Feces - chemistry ; Feces - microbiology ; Ferrosoferric Oxide - chemistry ; Fluorescence ; Fluorescent Dyes - chemistry ; Humans ; Magnetite Nanoparticles - chemistry ; Nanomedicine - methods ; Remote Sensing Technology - methods ; SciAdv r-articles ; Sensitivity and Specificity ; Spores, Bacterial</subject><ispartof>Science advances, 2019-01, Vol.5 (1), p.eaau9650</ispartof><rights>Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 2019 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-d79869edb9f77127476ec6df18e9300afd32fa42263659924e683805d8dc329e3</citedby><cites>FETCH-LOGICAL-c390t-d79869edb9f77127476ec6df18e9300afd32fa42263659924e683805d8dc329e3</cites><orcidid>0000-0003-3380-2870 ; 0000-0001-9292-112X ; 0000-0003-4804-1680 ; 0000-0003-3125-5199 ; 0000-0001-5820-0089 ; 0000-0002-5757-7885 ; 0000-0003-1291-6537 ; 0000-0003-1152-8962</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357761/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357761/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30746470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yabin</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Yang, Lidong</creatorcontrib><creatorcontrib>Vong, Chi Ian</creatorcontrib><creatorcontrib>Chan, Kai Fung</creatorcontrib><creatorcontrib>Wu, William K K</creatorcontrib><creatorcontrib>Kwong, Thomas N Y</creatorcontrib><creatorcontrib>Lo, Norman W S</creatorcontrib><creatorcontrib>Ip, Margaret</creatorcontrib><creatorcontrib>Wong, Sunny H</creatorcontrib><creatorcontrib>Sung, Joseph J Y</creatorcontrib><creatorcontrib>Chiu, Philip W Y</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><title>Real-time tracking of fluorescent magnetic spore-based microrobots for remote detection of C. diff toxins</title><title>Science advances</title><addtitle>Sci Adv</addtitle><description>A rapid, direct, and low-cost method for detecting bacterial toxins associated with common gastrointestinal diseases remains a great challenge despite numerous studies and clinical assays. Motion-based detection through tracking the emerging micro- and nanorobots has shown great potential in chemo- and biosensing due to accelerated "chemistry on the move". Here, we described the use of fluorescent magnetic spore-based microrobots (FMSMs) as a highly efficient mobile sensing platform for the detection of toxins secreted by
(
) that were present in patients' stool. These microrobots were synthesized rapidly and inexpensively by the direct deposition of magnetic nanoparticles and the subsequent encapsulation of sensing probes on the porous natural spores. Because of the cooperation effect of natural spore, magnetic Fe
O
nanoparticles, and functionalized carbon nanodots, selective fluorescence detection of the prepared FMSMs is demonstrated in
bacterial supernatant and even in actual clinical stool samples from infectious patients within tens of minutes, suggesting rapid response and good selectivity and sensitivity of FMSMs toward
toxins.</description><subject>Applied Sciences and Engineering</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Toxins - analysis</subject><subject>Carbon - chemistry</subject><subject>Clostridium difficile - physiology</subject><subject>Clostridium Infections - diagnosis</subject><subject>Clostridium Infections - microbiology</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Ferrosoferric Oxide - chemistry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Nanomedicine - methods</subject><subject>Remote Sensing Technology - methods</subject><subject>SciAdv r-articles</subject><subject>Sensitivity and Specificity</subject><subject>Spores, Bacterial</subject><issn>2375-2548</issn><issn>2375-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkFtLAzEQhYMottS--ij5A1tz2U02L4IUb1AQRJ-XbDKp0d1NSdKi_96WVqlPM8zM-YZzELqkZEYpE9fJeG03M63XSlTkBI0Zl1XBqrI-PepHaJrSByGElkJUVJ2jESeyFKUkY-RfQHdF9j3gHLX59MMSB4ddtw4RkoEh414vB8je4LTazopWJ7C49yaGGNqQE3Yh4gh9yIAtZDDZh2EHmc-w9c7hHL78kC7QmdNdgumhTtDb_d3r_LFYPD88zW8XheGK5MJKVQsFtlVOSspkKQUYYR2tQXFCtLOcOV0yJriolGIliJrXpLK1NZwp4BN0s-eu1m0Pdmch6q5ZRd_r-N0E7Zv_m8G_N8uwaQSvpBR0C5jtAVuHKUVwf1pKml3uzT735pD7VnB1_PHv_Ddl_gOnUIMG</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Zhang, Yabin</creator><creator>Zhang, Lin</creator><creator>Yang, Lidong</creator><creator>Vong, Chi Ian</creator><creator>Chan, Kai Fung</creator><creator>Wu, William K K</creator><creator>Kwong, Thomas N Y</creator><creator>Lo, Norman W S</creator><creator>Ip, Margaret</creator><creator>Wong, Sunny H</creator><creator>Sung, Joseph J Y</creator><creator>Chiu, Philip W Y</creator><creator>Zhang, Li</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3380-2870</orcidid><orcidid>https://orcid.org/0000-0001-9292-112X</orcidid><orcidid>https://orcid.org/0000-0003-4804-1680</orcidid><orcidid>https://orcid.org/0000-0003-3125-5199</orcidid><orcidid>https://orcid.org/0000-0001-5820-0089</orcidid><orcidid>https://orcid.org/0000-0002-5757-7885</orcidid><orcidid>https://orcid.org/0000-0003-1291-6537</orcidid><orcidid>https://orcid.org/0000-0003-1152-8962</orcidid></search><sort><creationdate>20190101</creationdate><title>Real-time tracking of fluorescent magnetic spore-based microrobots for remote detection of C. diff toxins</title><author>Zhang, Yabin ; Zhang, Lin ; Yang, Lidong ; Vong, Chi Ian ; Chan, Kai Fung ; Wu, William K K ; Kwong, Thomas N Y ; Lo, Norman W S ; Ip, Margaret ; Wong, Sunny H ; Sung, Joseph J Y ; Chiu, Philip W Y ; Zhang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-d79869edb9f77127476ec6df18e9300afd32fa42263659924e683805d8dc329e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Applied Sciences and Engineering</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Toxins - analysis</topic><topic>Carbon - chemistry</topic><topic>Clostridium difficile - physiology</topic><topic>Clostridium Infections - diagnosis</topic><topic>Clostridium Infections - microbiology</topic><topic>Feces - chemistry</topic><topic>Feces - microbiology</topic><topic>Ferrosoferric Oxide - chemistry</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Humans</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Nanomedicine - methods</topic><topic>Remote Sensing Technology - methods</topic><topic>SciAdv r-articles</topic><topic>Sensitivity and Specificity</topic><topic>Spores, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yabin</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Yang, Lidong</creatorcontrib><creatorcontrib>Vong, Chi Ian</creatorcontrib><creatorcontrib>Chan, Kai Fung</creatorcontrib><creatorcontrib>Wu, William K K</creatorcontrib><creatorcontrib>Kwong, Thomas N Y</creatorcontrib><creatorcontrib>Lo, Norman W S</creatorcontrib><creatorcontrib>Ip, Margaret</creatorcontrib><creatorcontrib>Wong, Sunny H</creatorcontrib><creatorcontrib>Sung, Joseph J Y</creatorcontrib><creatorcontrib>Chiu, Philip W Y</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Science advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yabin</au><au>Zhang, Lin</au><au>Yang, Lidong</au><au>Vong, Chi Ian</au><au>Chan, Kai Fung</au><au>Wu, William K K</au><au>Kwong, Thomas N Y</au><au>Lo, Norman W S</au><au>Ip, Margaret</au><au>Wong, Sunny H</au><au>Sung, Joseph J Y</au><au>Chiu, Philip W Y</au><au>Zhang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-time tracking of fluorescent magnetic spore-based microrobots for remote detection of C. diff toxins</atitle><jtitle>Science advances</jtitle><addtitle>Sci Adv</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>5</volume><issue>1</issue><spage>eaau9650</spage><pages>eaau9650-</pages><issn>2375-2548</issn><eissn>2375-2548</eissn><abstract>A rapid, direct, and low-cost method for detecting bacterial toxins associated with common gastrointestinal diseases remains a great challenge despite numerous studies and clinical assays. Motion-based detection through tracking the emerging micro- and nanorobots has shown great potential in chemo- and biosensing due to accelerated "chemistry on the move". Here, we described the use of fluorescent magnetic spore-based microrobots (FMSMs) as a highly efficient mobile sensing platform for the detection of toxins secreted by
(
) that were present in patients' stool. These microrobots were synthesized rapidly and inexpensively by the direct deposition of magnetic nanoparticles and the subsequent encapsulation of sensing probes on the porous natural spores. Because of the cooperation effect of natural spore, magnetic Fe
O
nanoparticles, and functionalized carbon nanodots, selective fluorescence detection of the prepared FMSMs is demonstrated in
bacterial supernatant and even in actual clinical stool samples from infectious patients within tens of minutes, suggesting rapid response and good selectivity and sensitivity of FMSMs toward
toxins.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>30746470</pmid><doi>10.1126/sciadv.aau9650</doi><orcidid>https://orcid.org/0000-0003-3380-2870</orcidid><orcidid>https://orcid.org/0000-0001-9292-112X</orcidid><orcidid>https://orcid.org/0000-0003-4804-1680</orcidid><orcidid>https://orcid.org/0000-0003-3125-5199</orcidid><orcidid>https://orcid.org/0000-0001-5820-0089</orcidid><orcidid>https://orcid.org/0000-0002-5757-7885</orcidid><orcidid>https://orcid.org/0000-0003-1291-6537</orcidid><orcidid>https://orcid.org/0000-0003-1152-8962</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Science advances, 2019-01, Vol.5 (1), p.eaau9650 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Applied Sciences and Engineering Bacterial Proteins - analysis Bacterial Toxins - analysis Carbon - chemistry Clostridium difficile - physiology Clostridium Infections - diagnosis Clostridium Infections - microbiology Feces - chemistry Feces - microbiology Ferrosoferric Oxide - chemistry Fluorescence Fluorescent Dyes - chemistry Humans Magnetite Nanoparticles - chemistry Nanomedicine - methods Remote Sensing Technology - methods SciAdv r-articles Sensitivity and Specificity Spores, Bacterial |
| title | Real-time tracking of fluorescent magnetic spore-based microrobots for remote detection of C. diff toxins |
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