Subculturing cells have no effect on CRISPR/Cas9‐mediated cleavage of UL30 gene in pseudorabies virus

CRISPR/Cas9‐mediated genome editing can inhibit virus infection by targeting the conserved regions of the viral genomic DNA. Unexpectedly, we found previously that pseudorabies virus (PRV) could escape from CRISPR/Cas9‐mediated inhibition. In order to elucidate whether the escape of PRV from Cas9‐me...

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Veröffentlicht in:	Animal models and experimental medicine 2018-03, Vol.1 (1), p.74-77
Hauptverfasser:	Ren, Lin‐zhu, Peng, Zhi‐yuan, Ouyang, Ting, Liu, Xiao‐hui, Chen, Xin‐rong, Ye, Li, Fan, Jun‐wen, Ouyang, Hong‐sheng, Pang, Da‐xin, Bai, Jie‐ying
Format:	Artikel
Sprache:	eng
Schlagworte:	CRISPR CRISPR/Cas9 Deoxyribonucleic acid DNA Genomes Genomic instability Infections Proteins pseudorabies virus (PRV) Short Communication single‐guide RNA (sgRNA) UL30 protein
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