Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats
The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function i...
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| Veröffentlicht in: | Neuroscience bulletin 2019-02, Vol.35 (1), p.67-78 |
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| creator | Deng, Yu Tan, Xing Li, Miao-Ling Wang, Wei-Zhong Wang, Yang-Kai |
| description | The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs. |
| doi_str_mv | 10.1007/s12264-018-0298-3 |
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It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.</description><identifier>ISSN: 1673-7067</identifier><identifier>ISSN: 1995-8218</identifier><identifier>EISSN: 1995-8218</identifier><identifier>DOI: 10.1007/s12264-018-0298-3</identifier><identifier>PMID: 30318562</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>ACE inhibitors ; Acetylcholine ; Acetylcholine - metabolism ; Anatomy ; Anesthesiology ; Angiotensin-Converting Enzyme 2 ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Blood Pressure - physiology ; Cardiovascular System - metabolism ; Cholinergic Neurons - metabolism ; Enzymes ; Ethylenediaminetetraacetic acid ; Fluorescence ; Heart beat ; Human Physiology ; Hypertension ; Hypertension - metabolism ; Male ; Neurology ; Neurosciences ; Original ; Original Article ; Pain Medicine ; Peptidyl-Dipeptidase A - metabolism ; Rats ; Rats, Inbred SHR - metabolism</subject><ispartof>Neuroscience bulletin, 2019-02, Vol.35 (1), p.67-78</ispartof><rights>Shanghai Institutes for Biological Sciences, CAS and Springer Nature Singapore Pte Ltd. 2018</rights><rights>COPYRIGHT 2019 Springer</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-8403ab27e47a541565c6b2cac82617ed0561db9773e6235247a20ca9951e2c533</citedby><cites>FETCH-LOGICAL-c509t-8403ab27e47a541565c6b2cac82617ed0561db9773e6235247a20ca9951e2c533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357273/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357273/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30318562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Yu</creatorcontrib><creatorcontrib>Tan, Xing</creatorcontrib><creatorcontrib>Li, Miao-Ling</creatorcontrib><creatorcontrib>Wang, Wei-Zhong</creatorcontrib><creatorcontrib>Wang, Yang-Kai</creatorcontrib><title>Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats</title><title>Neuroscience bulletin</title><addtitle>Neurosci. Bull</addtitle><addtitle>Neurosci Bull</addtitle><description>The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.</description><subject>ACE inhibitors</subject><subject>Acetylcholine</subject><subject>Acetylcholine - metabolism</subject><subject>Anatomy</subject><subject>Anesthesiology</subject><subject>Angiotensin-Converting Enzyme 2</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood Pressure - physiology</subject><subject>Cardiovascular System - metabolism</subject><subject>Cholinergic Neurons - metabolism</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fluorescence</subject><subject>Heart beat</subject><subject>Human Physiology</subject><subject>Hypertension</subject><subject>Hypertension - metabolism</subject><subject>Male</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Original</subject><subject>Original Article</subject><subject>Pain Medicine</subject><subject>Peptidyl-Dipeptidase A - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred SHR - metabolism</subject><issn>1673-7067</issn><issn>1995-8218</issn><issn>1995-8218</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks9u1DAQxiMEoqXwAFyQJS5cUvwntpML0ioqLVIR0ha4Wl5nNusqsRc7WWl5IJ6zNikVlRDywfb4N9-M7a8oXhN8TjCW7yOhVFQlJnWJaVOX7ElxSpqGlzUl9dO0FpKVEgt5UryI8RZjgSWrnhcnDDNSc0FPi18r11s_gYvWla13BwiTdT26cD-PIyCKrEPTDtDaxynoAX0HNwU_6Any7jN08zBotIZ-zrGI2p0frIPQW4NubO_0kNW061CrQ2f9QUeT0PA7dHMc9zqpTwleQ9x7F5NEqnh13Kc-clOHVFpP8WXxbKuHCK_u57Pi28eLr-1Vef3l8lO7ui4Nx81U1hVmekMlVFLzinDBjdhQo01NBZHQYS5It2mkZCAo4zRhFBudnowANZyxs-LDorufNyN0Jt9WD2of7KjDUXlt1eMTZ3eq9wclGJdUZoF39wLB_5ghTmq00UB6JAd-jooSiikmEjcJfbugvR5AWbf1SdFkXK0kkQ2VhNNEnf-DSqOD0RrvYGtT_FECWRJM8DEG2D50T7DKtlGLbVSyjcq2UbnrN39f-yHjj08SQBcgpiPXQ1C3fg7pd-N_VO8AfsLQNg</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Deng, Yu</creator><creator>Tan, Xing</creator><creator>Li, Miao-Ling</creator><creator>Wang, Wei-Zhong</creator><creator>Wang, Yang-Kai</creator><general>Springer Singapore</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190201</creationdate><title>Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats</title><author>Deng, Yu ; Tan, Xing ; Li, Miao-Ling ; Wang, Wei-Zhong ; Wang, Yang-Kai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-8403ab27e47a541565c6b2cac82617ed0561db9773e6235247a20ca9951e2c533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>ACE inhibitors</topic><topic>Acetylcholine</topic><topic>Acetylcholine - metabolism</topic><topic>Anatomy</topic><topic>Anesthesiology</topic><topic>Angiotensin-Converting Enzyme 2</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood Pressure - physiology</topic><topic>Cardiovascular System - metabolism</topic><topic>Cholinergic Neurons - metabolism</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fluorescence</topic><topic>Heart beat</topic><topic>Human Physiology</topic><topic>Hypertension</topic><topic>Hypertension - metabolism</topic><topic>Male</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Original</topic><topic>Original Article</topic><topic>Pain Medicine</topic><topic>Peptidyl-Dipeptidase A - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred SHR - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Yu</creatorcontrib><creatorcontrib>Tan, Xing</creatorcontrib><creatorcontrib>Li, Miao-Ling</creatorcontrib><creatorcontrib>Wang, Wei-Zhong</creatorcontrib><creatorcontrib>Wang, Yang-Kai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuroscience bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Yu</au><au>Tan, Xing</au><au>Li, Miao-Ling</au><au>Wang, Wei-Zhong</au><au>Wang, Yang-Kai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats</atitle><jtitle>Neuroscience bulletin</jtitle><stitle>Neurosci. Bull</stitle><addtitle>Neurosci Bull</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>35</volume><issue>1</issue><spage>67</spage><epage>78</epage><pages>67-78</pages><issn>1673-7067</issn><issn>1995-8218</issn><eissn>1995-8218</eissn><abstract>The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>30318562</pmid><doi>10.1007/s12264-018-0298-3</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuroscience bulletin, 2019-02, Vol.35 (1), p.67-78 |
| issn | 1673-7067 1995-8218 1995-8218 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | ACE inhibitors Acetylcholine Acetylcholine - metabolism Anatomy Anesthesiology Angiotensin-Converting Enzyme 2 Animals Biomedical and Life Sciences Biomedicine Blood Pressure - physiology Cardiovascular System - metabolism Cholinergic Neurons - metabolism Enzymes Ethylenediaminetetraacetic acid Fluorescence Heart beat Human Physiology Hypertension Hypertension - metabolism Male Neurology Neurosciences Original Original Article Pain Medicine Peptidyl-Dipeptidase A - metabolism Rats Rats, Inbred SHR - metabolism |
| title | Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats |
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