A new full-length circular DNA sequencing method for viral-sized genomes reveals that RNAi transgenic plants provoke a shift in geminivirus populations in the field

Abstract We present a new method, CIDER-Seq (Circular DNA Enrichment sequencing) for the unbiased enrichment and long-read sequencing of viral-sized circular DNA molecules. We used CIDER-Seq to produce single-read full-length virus genomes for the first time. CIDER-Seq combines PCR-free virus enrich...

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Veröffentlicht in:	Nucleic acids research 2019-01, Vol.47 (2), p.e9-e9
Hauptverfasser:	Mehta, Devang, Hirsch-Hoffmann, Matthias, Were, Mariam, Patrignani, Andrea, Zaidi, Syed Shan-e-Ali, Were, Hassan, Gruissem, Wilhelm, Vanderschuren, Hervé
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Biochemistry, biophysics & molecular biology Biochimie, biophysique & biologie moléculaire DNA, Circular - chemistry DNA, Viral - chemistry Geminiviridae - genetics Genome, Viral High-Throughput Nucleotide Sequencing - methods Life sciences Methods Online Plants, Genetically Modified - genetics Plants, Genetically Modified - virology RNA Interference Sciences du vivant Sequence Analysis, DNA - methods
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