Novel non-phosphorylative pathway of pentose metabolism from bacteria
Pentoses, including D-xylose, L-arabinose, and D-arabinose, are generally phosphorylated to D-xylulose 5-phosphate in bacteria and fungi. However, in non-phosphorylative pathways analogous to the Entner-Dodoroff pathway in bacteria and archaea, such pentoses can be converted to pyruvate and glycolal...
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| Veröffentlicht in: | Scientific reports 2019-01, Vol.9 (1), p.155-155, Article 155 |
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| creator | Watanabe, Seiya Fukumori, Fumiyasu Nishiwaki, Hisashi Sakurai, Yasuhiro Tajima, Kunihiko Watanabe, Yasuo |
| description | Pentoses, including D-xylose, L-arabinose, and D-arabinose, are generally phosphorylated to D-xylulose 5-phosphate in bacteria and fungi. However, in non-phosphorylative pathways analogous to the Entner-Dodoroff pathway in bacteria and archaea, such pentoses can be converted to pyruvate and glycolaldehyde (Route I) or α-ketoglutarate (Route II) via a 2-keto-3-deoxypentonate (KDP) intermediate. Putative gene clusters related to these metabolic pathways were identified on the genome of
Herbaspirillum huttiense
IAM 15032 using a bioinformatic analysis. The biochemical characterization of C785_RS13685, one of the components encoded to D-arabinonate dehydratase, differed from the known acid-sugar dehydratases. The biochemical characterization of the remaining components and a genetic expression analysis revealed that D- and L-KDP were converted not only to α-ketoglutarate, but also pyruvate and glycolate through the participation of dehydrogenase and hydrolase (Route III). Further analyses revealed that the Route II pathway of D-arabinose metabolism was not evolutionally related to the analogous pathway from archaea. |
| doi_str_mv | 10.1038/s41598-018-36774-6 |
| format | Article |
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Herbaspirillum huttiense
IAM 15032 using a bioinformatic analysis. The biochemical characterization of C785_RS13685, one of the components encoded to D-arabinonate dehydratase, differed from the known acid-sugar dehydratases. The biochemical characterization of the remaining components and a genetic expression analysis revealed that D- and L-KDP were converted not only to α-ketoglutarate, but also pyruvate and glycolate through the participation of dehydrogenase and hydrolase (Route III). Further analyses revealed that the Route II pathway of D-arabinose metabolism was not evolutionally related to the analogous pathway from archaea.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-36774-6</identifier><identifier>PMID: 30655589</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 631/326/41 ; 631/45/173 ; 82/80 ; 82/83 ; Arabinonate dehydratase ; Arabinose ; Bacteria ; Bacterial Proteins - metabolism ; Computational Biology - methods ; D-Xylulose 5-phosphate ; Fungi ; Gene clusters ; Genetic analysis ; Genomes ; Glycolaldehyde ; Herbaspirillum - genetics ; Herbaspirillum - metabolism ; Humanities and Social Sciences ; Hydrolase ; Ketoglutaric acid ; Metabolic Networks and Pathways ; Metabolic pathways ; Metabolism ; multidisciplinary ; Multigene Family ; Pentoses - metabolism ; Phosphorylation ; Pyruvic acid ; Science ; Science (multidisciplinary) ; Sugar ; Xylose ; Xylulose</subject><ispartof>Scientific reports, 2019-01, Vol.9 (1), p.155-155, Article 155</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-97fb90dc632585ebc1d4fd85e72b7f764c4e8da607508cb914b2d82b42d18bf63</citedby><cites>FETCH-LOGICAL-c577t-97fb90dc632585ebc1d4fd85e72b7f764c4e8da607508cb914b2d82b42d18bf63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336799/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336799/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30655589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Seiya</creatorcontrib><creatorcontrib>Fukumori, Fumiyasu</creatorcontrib><creatorcontrib>Nishiwaki, Hisashi</creatorcontrib><creatorcontrib>Sakurai, Yasuhiro</creatorcontrib><creatorcontrib>Tajima, Kunihiko</creatorcontrib><creatorcontrib>Watanabe, Yasuo</creatorcontrib><title>Novel non-phosphorylative pathway of pentose metabolism from bacteria</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Pentoses, including D-xylose, L-arabinose, and D-arabinose, are generally phosphorylated to D-xylulose 5-phosphate in bacteria and fungi. However, in non-phosphorylative pathways analogous to the Entner-Dodoroff pathway in bacteria and archaea, such pentoses can be converted to pyruvate and glycolaldehyde (Route I) or α-ketoglutarate (Route II) via a 2-keto-3-deoxypentonate (KDP) intermediate. Putative gene clusters related to these metabolic pathways were identified on the genome of
Herbaspirillum huttiense
IAM 15032 using a bioinformatic analysis. The biochemical characterization of C785_RS13685, one of the components encoded to D-arabinonate dehydratase, differed from the known acid-sugar dehydratases. The biochemical characterization of the remaining components and a genetic expression analysis revealed that D- and L-KDP were converted not only to α-ketoglutarate, but also pyruvate and glycolate through the participation of dehydrogenase and hydrolase (Route III). 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However, in non-phosphorylative pathways analogous to the Entner-Dodoroff pathway in bacteria and archaea, such pentoses can be converted to pyruvate and glycolaldehyde (Route I) or α-ketoglutarate (Route II) via a 2-keto-3-deoxypentonate (KDP) intermediate. Putative gene clusters related to these metabolic pathways were identified on the genome of
Herbaspirillum huttiense
IAM 15032 using a bioinformatic analysis. The biochemical characterization of C785_RS13685, one of the components encoded to D-arabinonate dehydratase, differed from the known acid-sugar dehydratases. The biochemical characterization of the remaining components and a genetic expression analysis revealed that D- and L-KDP were converted not only to α-ketoglutarate, but also pyruvate and glycolate through the participation of dehydrogenase and hydrolase (Route III). Further analyses revealed that the Route II pathway of D-arabinose metabolism was not evolutionally related to the analogous pathway from archaea.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30655589</pmid><doi>10.1038/s41598-018-36774-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 38/39 631/326/41 631/45/173 82/80 82/83 Arabinonate dehydratase Arabinose Bacteria Bacterial Proteins - metabolism Computational Biology - methods D-Xylulose 5-phosphate Fungi Gene clusters Genetic analysis Genomes Glycolaldehyde Herbaspirillum - genetics Herbaspirillum - metabolism Humanities and Social Sciences Hydrolase Ketoglutaric acid Metabolic Networks and Pathways Metabolic pathways Metabolism multidisciplinary Multigene Family Pentoses - metabolism Phosphorylation Pyruvic acid Science Science (multidisciplinary) Sugar Xylose Xylulose |
| title | Novel non-phosphorylative pathway of pentose metabolism from bacteria |
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