Effect of Alkaloid Extract from African Jointfir (Gnetum africanum) Leaves on Manganese-Induced Toxicity in Drosophila melanogaster
Metal-induced toxicity in fruit fly (Drosophila melanogaster) is one of the established models for studying neurotoxicity and neurodegenerative diseases. Phytochemicals, especially alkaloids, have been reported to exhibit neuroprotection. Here, we assessed the protective effect of alkaloid extract f...
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| creator | Atoki, Victor Ayomide Awonyemi, Olatunde Isaac Ogunsuyi, Opeyemi Babatunde Oboh, Ganiyu |
| description | Metal-induced toxicity in fruit fly (Drosophila melanogaster) is one of the established models for studying neurotoxicity and neurodegenerative diseases. Phytochemicals, especially alkaloids, have been reported to exhibit neuroprotection. Here, we assessed the protective effect of alkaloid extract from African Jointfir (Gnetum africanum) leaf on manganese- (Mn-) induced toxicity in wild type fruit fly. Flies were exposed to 10 mM Mn, the alkaloid extract and cotreatment of Mn plus extract, respectively. The survival rate and locomotor performance of the flies were assessed 5 days posttreatment, at which point the flies were homogenized and assayed for acetylcholinesterase (AChE) activity, nitric oxide (NO), and reactive oxygen species (ROS) levels. Results showed that the extract significantly reverted Mn-induced reduction in the survival rate and locomotor performance of the flies. Furthermore, the extract counteracted the Mn-induced elevation in AChE activity, NO, and ROS levels. The alkaloid extract of the African Jointfir leaf may hence be a source of useful phytochemicals for the development of novel therapies for the management of neurodegeneration. |
| doi_str_mv | 10.1155/2018/8952646 |
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B. ; Francisco J B Mendonça Júnior</contributor><creatorcontrib>Atoki, Victor Ayomide ; Awonyemi, Olatunde Isaac ; Ogunsuyi, Opeyemi Babatunde ; Oboh, Ganiyu ; Mendonça Júnior, Francisco J. B. ; Francisco J B Mendonça Júnior</creatorcontrib><description>Metal-induced toxicity in fruit fly (Drosophila melanogaster) is one of the established models for studying neurotoxicity and neurodegenerative diseases. Phytochemicals, especially alkaloids, have been reported to exhibit neuroprotection. Here, we assessed the protective effect of alkaloid extract from African Jointfir (Gnetum africanum) leaf on manganese- (Mn-) induced toxicity in wild type fruit fly. Flies were exposed to 10 mM Mn, the alkaloid extract and cotreatment of Mn plus extract, respectively. The survival rate and locomotor performance of the flies were assessed 5 days posttreatment, at which point the flies were homogenized and assayed for acetylcholinesterase (AChE) activity, nitric oxide (NO), and reactive oxygen species (ROS) levels. Results showed that the extract significantly reverted Mn-induced reduction in the survival rate and locomotor performance of the flies. Furthermore, the extract counteracted the Mn-induced elevation in AChE activity, NO, and ROS levels. The alkaloid extract of the African Jointfir leaf may hence be a source of useful phytochemicals for the development of novel therapies for the management of neurodegeneration.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2018/8952646</identifier><identifier>PMID: 30693067</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Antioxidants - pharmacology ; Biochemistry ; Brain research ; Disease ; Drosophila melanogaster - drug effects ; Drosophila melanogaster - growth & development ; Drosophila melanogaster - metabolism ; Enzymes ; Gnetum - chemistry ; Insects ; Laboratories ; Leaves ; Manganese - toxicity ; Neurodegeneration ; Neuroprotective Agents - pharmacology ; Neurosciences ; Neurotoxicity ; Nitric oxide ; Oxidative stress ; Oxidative Stress - drug effects ; Phytochemicals ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Potassium ; Reactive Oxygen Species - metabolism</subject><ispartof>Oxidative medicine and cellular longevity, 2018-01, Vol.2018 (2018), p.1-10</ispartof><rights>Copyright © 2018 Ganiyu Oboh et al.</rights><rights>Copyright © 2018 Ganiyu Oboh et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2018 Ganiyu Oboh et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-5627678d694165d4ada5f65b470d3de65677a9b20d7143e4f4dbbe7509898a193</citedby><cites>FETCH-LOGICAL-c537t-5627678d694165d4ada5f65b470d3de65677a9b20d7143e4f4dbbe7509898a193</cites><orcidid>0000-0001-5167-9779 ; 0000-0002-1708-3864 ; 0000-0003-3075-1086</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332884/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332884/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30693067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mendonça Júnior, Francisco J. 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Flies were exposed to 10 mM Mn, the alkaloid extract and cotreatment of Mn plus extract, respectively. The survival rate and locomotor performance of the flies were assessed 5 days posttreatment, at which point the flies were homogenized and assayed for acetylcholinesterase (AChE) activity, nitric oxide (NO), and reactive oxygen species (ROS) levels. Results showed that the extract significantly reverted Mn-induced reduction in the survival rate and locomotor performance of the flies. Furthermore, the extract counteracted the Mn-induced elevation in AChE activity, NO, and ROS levels. 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Awonyemi, Olatunde Isaac ; Ogunsuyi, Opeyemi Babatunde ; Oboh, Ganiyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-5627678d694165d4ada5f65b470d3de65677a9b20d7143e4f4dbbe7509898a193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Biochemistry</topic><topic>Brain research</topic><topic>Disease</topic><topic>Drosophila melanogaster - drug effects</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila melanogaster - metabolism</topic><topic>Enzymes</topic><topic>Gnetum - chemistry</topic><topic>Insects</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Manganese - toxicity</topic><topic>Neurodegeneration</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Neurosciences</topic><topic>Neurotoxicity</topic><topic>Nitric oxide</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Phytochemicals</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Potassium</topic><topic>Reactive Oxygen Species - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atoki, Victor Ayomide</creatorcontrib><creatorcontrib>Awonyemi, Olatunde Isaac</creatorcontrib><creatorcontrib>Ogunsuyi, Opeyemi Babatunde</creatorcontrib><creatorcontrib>Oboh, Ganiyu</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oxidative medicine and cellular longevity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atoki, Victor Ayomide</au><au>Awonyemi, Olatunde Isaac</au><au>Ogunsuyi, Opeyemi Babatunde</au><au>Oboh, Ganiyu</au><au>Mendonça Júnior, Francisco J. B.</au><au>Francisco J B Mendonça Júnior</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Alkaloid Extract from African Jointfir (Gnetum africanum) Leaves on Manganese-Induced Toxicity in Drosophila melanogaster</atitle><jtitle>Oxidative medicine and cellular longevity</jtitle><addtitle>Oxid Med Cell Longev</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1942-0900</issn><eissn>1942-0994</eissn><abstract>Metal-induced toxicity in fruit fly (Drosophila melanogaster) is one of the established models for studying neurotoxicity and neurodegenerative diseases. Phytochemicals, especially alkaloids, have been reported to exhibit neuroprotection. Here, we assessed the protective effect of alkaloid extract from African Jointfir (Gnetum africanum) leaf on manganese- (Mn-) induced toxicity in wild type fruit fly. Flies were exposed to 10 mM Mn, the alkaloid extract and cotreatment of Mn plus extract, respectively. The survival rate and locomotor performance of the flies were assessed 5 days posttreatment, at which point the flies were homogenized and assayed for acetylcholinesterase (AChE) activity, nitric oxide (NO), and reactive oxygen species (ROS) levels. Results showed that the extract significantly reverted Mn-induced reduction in the survival rate and locomotor performance of the flies. Furthermore, the extract counteracted the Mn-induced elevation in AChE activity, NO, and ROS levels. The alkaloid extract of the African Jointfir leaf may hence be a source of useful phytochemicals for the development of novel therapies for the management of neurodegeneration.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>30693067</pmid><doi>10.1155/2018/8952646</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5167-9779</orcidid><orcidid>https://orcid.org/0000-0002-1708-3864</orcidid><orcidid>https://orcid.org/0000-0003-3075-1086</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antioxidants - pharmacology Biochemistry Brain research Disease Drosophila melanogaster - drug effects Drosophila melanogaster - growth & development Drosophila melanogaster - metabolism Enzymes Gnetum - chemistry Insects Laboratories Leaves Manganese - toxicity Neurodegeneration Neuroprotective Agents - pharmacology Neurosciences Neurotoxicity Nitric oxide Oxidative stress Oxidative Stress - drug effects Phytochemicals Plant Extracts - pharmacology Plant Leaves - chemistry Potassium Reactive Oxygen Species - metabolism |
| title | Effect of Alkaloid Extract from African Jointfir (Gnetum africanum) Leaves on Manganese-Induced Toxicity in Drosophila melanogaster |
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