Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations
The synaptic protein SHANK3 encodes a multidomain scaffold protein expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified de novo SHANK3 mutations in patients with autism spectrum disorders (ASD) and showed that SHANK3 represents one of the major genes for AS...
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| Veröffentlicht in: | Scientific reports 2019-01, Vol.9 (1), p.94-94, Article 94 |
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| creator | Gouder, Laura Vitrac, Aline Goubran-Botros, Hany Danckaert, Anne Tinevez, Jean-Yves André-Leroux, Gwenaëlle Atanasova, Ekaterina Lemière, Nathalie Biton, Anne Leblond, Claire S. Poulet, Aurélie Boland, Anne Deleuze, Jean-François Benchoua, Alexandra Delorme, Richard Bourgeron, Thomas Cloëz-Tayarani, Isabelle |
| description | The synaptic protein SHANK3 encodes a multidomain scaffold protein expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified
de novo SHANK3
mutations in patients with autism spectrum disorders (ASD) and showed that
SHANK3
represents one of the major genes for ASD. Here, we analyzed the pyramidal cortical neurons derived from induced pluripotent stem cells from four patients with ASD carrying
SHANK3 de novo
truncating mutations. At 40–45 days after the differentiation of neural stem cells, dendritic spines from pyramidal neurons presented variable morphologies: filopodia, thin, stubby and muschroom, as measured in 3D using GFP labeling and immunofluorescence. As compared to three controls, we observed a significant decrease in
SHANK3
mRNA levels (less than 50% of controls) in correlation with a significant reduction in dendritic spine densities and whole spine and spine head volumes. These results, obtained through the analysis of
de novo SHANK3
mutations in the patients’ genomic background, provide further support for the presence of synaptic abnormalities in a subset of patients with ASD. |
| doi_str_mv | 10.1038/s41598-018-36993-x |
| format | Article |
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de novo SHANK3
mutations in patients with autism spectrum disorders (ASD) and showed that
SHANK3
represents one of the major genes for ASD. Here, we analyzed the pyramidal cortical neurons derived from induced pluripotent stem cells from four patients with ASD carrying
SHANK3 de novo
truncating mutations. At 40–45 days after the differentiation of neural stem cells, dendritic spines from pyramidal neurons presented variable morphologies: filopodia, thin, stubby and muschroom, as measured in 3D using GFP labeling and immunofluorescence. As compared to three controls, we observed a significant decrease in
SHANK3
mRNA levels (less than 50% of controls) in correlation with a significant reduction in dendritic spine densities and whole spine and spine head volumes. These results, obtained through the analysis of
de novo SHANK3
mutations in the patients’ genomic background, provide further support for the presence of synaptic abnormalities in a subset of patients with ASD.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-36993-x</identifier><identifier>PMID: 30643170</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 13/106 ; 13/44 ; 13/51 ; 14/19 ; 38 ; 38/39 ; 38/90 ; 45/91 ; 631/378/2597 ; 631/45/500 ; 631/80 ; Autism ; Autistic Disorder - genetics ; Autistic Disorder - pathology ; Cell Differentiation ; Dendrites - pathology ; Dendritic spines ; Filopodia ; Humanities and Social Sciences ; Humans ; Immunofluorescence ; Induced Pluripotent Stem Cells - physiology ; Life Sciences ; Microscopy, Fluorescence ; mRNA ; multidisciplinary ; Mutation ; Nerve Tissue Proteins - deficiency ; Nerve Tissue Proteins - genetics ; Neural stem cells ; Pluripotency ; Postsynaptic density ; Pyramidal cells ; Pyramidal Cells - cytology ; Pyramidal Cells - pathology ; Science ; Science (multidisciplinary) ; Sequence Deletion ; Spine ; Stem cells ; Synapses</subject><ispartof>Scientific reports, 2019-01, Vol.9 (1), p.94-94, Article 94</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-1f28620246b518d08b00c91dd996e305190bdc9c0a56623110e55ecf9787ae1b3</citedby><cites>FETCH-LOGICAL-c508t-1f28620246b518d08b00c91dd996e305190bdc9c0a56623110e55ecf9787ae1b3</cites><orcidid>0000-0002-0998-4718 ; 0000-0001-6832-9812 ; 0000-0002-9824-6580 ; 0000-0002-5358-4463 ; 0000-0001-8164-9220 ; 0000-0001-8984-643X ; 0000-0002-9980-6471 ; 0000-0001-8789-5676 ; 0000-0002-3814-4195</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331634/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331634/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30643170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02010139$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gouder, Laura</creatorcontrib><creatorcontrib>Vitrac, Aline</creatorcontrib><creatorcontrib>Goubran-Botros, Hany</creatorcontrib><creatorcontrib>Danckaert, Anne</creatorcontrib><creatorcontrib>Tinevez, Jean-Yves</creatorcontrib><creatorcontrib>André-Leroux, Gwenaëlle</creatorcontrib><creatorcontrib>Atanasova, Ekaterina</creatorcontrib><creatorcontrib>Lemière, Nathalie</creatorcontrib><creatorcontrib>Biton, Anne</creatorcontrib><creatorcontrib>Leblond, Claire S.</creatorcontrib><creatorcontrib>Poulet, Aurélie</creatorcontrib><creatorcontrib>Boland, Anne</creatorcontrib><creatorcontrib>Deleuze, Jean-François</creatorcontrib><creatorcontrib>Benchoua, Alexandra</creatorcontrib><creatorcontrib>Delorme, Richard</creatorcontrib><creatorcontrib>Bourgeron, Thomas</creatorcontrib><creatorcontrib>Cloëz-Tayarani, Isabelle</creatorcontrib><title>Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The synaptic protein SHANK3 encodes a multidomain scaffold protein expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified
de novo SHANK3
mutations in patients with autism spectrum disorders (ASD) and showed that
SHANK3
represents one of the major genes for ASD. Here, we analyzed the pyramidal cortical neurons derived from induced pluripotent stem cells from four patients with ASD carrying
SHANK3 de novo
truncating mutations. At 40–45 days after the differentiation of neural stem cells, dendritic spines from pyramidal neurons presented variable morphologies: filopodia, thin, stubby and muschroom, as measured in 3D using GFP labeling and immunofluorescence. As compared to three controls, we observed a significant decrease in
SHANK3
mRNA levels (less than 50% of controls) in correlation with a significant reduction in dendritic spine densities and whole spine and spine head volumes. These results, obtained through the analysis of
de novo SHANK3
mutations in the patients’ genomic background, provide further support for the presence of synaptic abnormalities in a subset of patients with ASD.</description><subject>13/100</subject><subject>13/106</subject><subject>13/44</subject><subject>13/51</subject><subject>14/19</subject><subject>38</subject><subject>38/39</subject><subject>38/90</subject><subject>45/91</subject><subject>631/378/2597</subject><subject>631/45/500</subject><subject>631/80</subject><subject>Autism</subject><subject>Autistic Disorder - genetics</subject><subject>Autistic Disorder - pathology</subject><subject>Cell Differentiation</subject><subject>Dendrites - pathology</subject><subject>Dendritic spines</subject><subject>Filopodia</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Induced Pluripotent Stem Cells - physiology</subject><subject>Life Sciences</subject><subject>Microscopy, Fluorescence</subject><subject>mRNA</subject><subject>multidisciplinary</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - deficiency</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Neural stem cells</subject><subject>Pluripotency</subject><subject>Postsynaptic density</subject><subject>Pyramidal cells</subject><subject>Pyramidal Cells - cytology</subject><subject>Pyramidal Cells - pathology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sequence Deletion</subject><subject>Spine</subject><subject>Stem cells</subject><subject>Synapses</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9ksFu1DAQhiMEolXpC3BAlrjAITBjx974grRaQRexAqTC2XIcZ9dVYm_tZGnfvi4ppfSAL7Y83_wz9vxF8RLhHQKr36cKuaxLwLpkQkpWXj0pjilUvKSM0qcPzkfFaUoXkBenskL5vDhiICqGCzguLpf9aKNtSdo7H7bW2-QScZ647-ersrXRHXLQhDg6o3vi7RSDT6SLYSB7PTrrx0R-uXFH9DS6NBCjY7x2fktaS3w4BHK-Xn79wsgwjRnPuS-KZ53ukz2920-Kn58-_lity823s8-r5aY0HOqxxI7WggKtRMOxbqFuAIzEtpVSWAYcJTStkQY0F4IyRLCcW9PJRb3QFht2UnyYdfdTM9jW5E6j7tU-ukHHaxW0U_9GvNupbTgowRgKVmWBt7PA7lHaerlRt3dAAQGZPGBm39wVi-FysmlUg0vG9r32NkxJUVxIVnGkkNHXj9CLMEWfvyJTYpFF8zQzRWfKxJBStN19Bwjq1gBqNoDKBlC_DaCuctKrh0--T_kz7gywGUg55Lc2_q39H9kbtoi78g</recordid><startdate>20190114</startdate><enddate>20190114</enddate><creator>Gouder, Laura</creator><creator>Vitrac, Aline</creator><creator>Goubran-Botros, Hany</creator><creator>Danckaert, Anne</creator><creator>Tinevez, Jean-Yves</creator><creator>André-Leroux, Gwenaëlle</creator><creator>Atanasova, Ekaterina</creator><creator>Lemière, Nathalie</creator><creator>Biton, Anne</creator><creator>Leblond, Claire S.</creator><creator>Poulet, Aurélie</creator><creator>Boland, Anne</creator><creator>Deleuze, Jean-François</creator><creator>Benchoua, Alexandra</creator><creator>Delorme, Richard</creator><creator>Bourgeron, Thomas</creator><creator>Cloëz-Tayarani, Isabelle</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0998-4718</orcidid><orcidid>https://orcid.org/0000-0001-6832-9812</orcidid><orcidid>https://orcid.org/0000-0002-9824-6580</orcidid><orcidid>https://orcid.org/0000-0002-5358-4463</orcidid><orcidid>https://orcid.org/0000-0001-8164-9220</orcidid><orcidid>https://orcid.org/0000-0001-8984-643X</orcidid><orcidid>https://orcid.org/0000-0002-9980-6471</orcidid><orcidid>https://orcid.org/0000-0001-8789-5676</orcidid><orcidid>https://orcid.org/0000-0002-3814-4195</orcidid></search><sort><creationdate>20190114</creationdate><title>Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations</title><author>Gouder, Laura ; Vitrac, Aline ; Goubran-Botros, Hany ; Danckaert, Anne ; Tinevez, Jean-Yves ; André-Leroux, Gwenaëlle ; Atanasova, Ekaterina ; Lemière, Nathalie ; Biton, Anne ; Leblond, Claire S. ; Poulet, Aurélie ; Boland, Anne ; Deleuze, Jean-François ; Benchoua, Alexandra ; Delorme, Richard ; Bourgeron, Thomas ; Cloëz-Tayarani, Isabelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-1f28620246b518d08b00c91dd996e305190bdc9c0a56623110e55ecf9787ae1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>13/100</topic><topic>13/106</topic><topic>13/44</topic><topic>13/51</topic><topic>14/19</topic><topic>38</topic><topic>38/39</topic><topic>38/90</topic><topic>45/91</topic><topic>631/378/2597</topic><topic>631/45/500</topic><topic>631/80</topic><topic>Autism</topic><topic>Autistic Disorder - genetics</topic><topic>Autistic Disorder - pathology</topic><topic>Cell Differentiation</topic><topic>Dendrites - pathology</topic><topic>Dendritic spines</topic><topic>Filopodia</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Induced Pluripotent Stem Cells - physiology</topic><topic>Life Sciences</topic><topic>Microscopy, Fluorescence</topic><topic>mRNA</topic><topic>multidisciplinary</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - deficiency</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Neural stem cells</topic><topic>Pluripotency</topic><topic>Postsynaptic density</topic><topic>Pyramidal cells</topic><topic>Pyramidal Cells - cytology</topic><topic>Pyramidal Cells - pathology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sequence Deletion</topic><topic>Spine</topic><topic>Stem cells</topic><topic>Synapses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gouder, Laura</creatorcontrib><creatorcontrib>Vitrac, Aline</creatorcontrib><creatorcontrib>Goubran-Botros, Hany</creatorcontrib><creatorcontrib>Danckaert, Anne</creatorcontrib><creatorcontrib>Tinevez, Jean-Yves</creatorcontrib><creatorcontrib>André-Leroux, Gwenaëlle</creatorcontrib><creatorcontrib>Atanasova, Ekaterina</creatorcontrib><creatorcontrib>Lemière, Nathalie</creatorcontrib><creatorcontrib>Biton, Anne</creatorcontrib><creatorcontrib>Leblond, Claire S.</creatorcontrib><creatorcontrib>Poulet, Aurélie</creatorcontrib><creatorcontrib>Boland, Anne</creatorcontrib><creatorcontrib>Deleuze, Jean-François</creatorcontrib><creatorcontrib>Benchoua, Alexandra</creatorcontrib><creatorcontrib>Delorme, Richard</creatorcontrib><creatorcontrib>Bourgeron, Thomas</creatorcontrib><creatorcontrib>Cloëz-Tayarani, Isabelle</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gouder, Laura</au><au>Vitrac, Aline</au><au>Goubran-Botros, Hany</au><au>Danckaert, Anne</au><au>Tinevez, Jean-Yves</au><au>André-Leroux, Gwenaëlle</au><au>Atanasova, Ekaterina</au><au>Lemière, Nathalie</au><au>Biton, Anne</au><au>Leblond, Claire S.</au><au>Poulet, Aurélie</au><au>Boland, Anne</au><au>Deleuze, Jean-François</au><au>Benchoua, Alexandra</au><au>Delorme, Richard</au><au>Bourgeron, Thomas</au><au>Cloëz-Tayarani, Isabelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-01-14</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>94</spage><epage>94</epage><pages>94-94</pages><artnum>94</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The synaptic protein SHANK3 encodes a multidomain scaffold protein expressed at the postsynaptic density of neuronal excitatory synapses. We previously identified
de novo SHANK3
mutations in patients with autism spectrum disorders (ASD) and showed that
SHANK3
represents one of the major genes for ASD. Here, we analyzed the pyramidal cortical neurons derived from induced pluripotent stem cells from four patients with ASD carrying
SHANK3 de novo
truncating mutations. At 40–45 days after the differentiation of neural stem cells, dendritic spines from pyramidal neurons presented variable morphologies: filopodia, thin, stubby and muschroom, as measured in 3D using GFP labeling and immunofluorescence. As compared to three controls, we observed a significant decrease in
SHANK3
mRNA levels (less than 50% of controls) in correlation with a significant reduction in dendritic spine densities and whole spine and spine head volumes. These results, obtained through the analysis of
de novo SHANK3
mutations in the patients’ genomic background, provide further support for the presence of synaptic abnormalities in a subset of patients with ASD.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30643170</pmid><doi>10.1038/s41598-018-36993-x</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0998-4718</orcidid><orcidid>https://orcid.org/0000-0001-6832-9812</orcidid><orcidid>https://orcid.org/0000-0002-9824-6580</orcidid><orcidid>https://orcid.org/0000-0002-5358-4463</orcidid><orcidid>https://orcid.org/0000-0001-8164-9220</orcidid><orcidid>https://orcid.org/0000-0001-8984-643X</orcidid><orcidid>https://orcid.org/0000-0002-9980-6471</orcidid><orcidid>https://orcid.org/0000-0001-8789-5676</orcidid><orcidid>https://orcid.org/0000-0002-3814-4195</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2019-01, Vol.9 (1), p.94-94, Article 94 |
| issn | 2045-2322 2045-2322 |
| language | eng |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13/100 13/106 13/44 13/51 14/19 38 38/39 38/90 45/91 631/378/2597 631/45/500 631/80 Autism Autistic Disorder - genetics Autistic Disorder - pathology Cell Differentiation Dendrites - pathology Dendritic spines Filopodia Humanities and Social Sciences Humans Immunofluorescence Induced Pluripotent Stem Cells - physiology Life Sciences Microscopy, Fluorescence mRNA multidisciplinary Mutation Nerve Tissue Proteins - deficiency Nerve Tissue Proteins - genetics Neural stem cells Pluripotency Postsynaptic density Pyramidal cells Pyramidal Cells - cytology Pyramidal Cells - pathology Science Science (multidisciplinary) Sequence Deletion Spine Stem cells Synapses |
| title | Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T13%3A47%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Altered%20spinogenesis%20in%20iPSC-derived%20cortical%20neurons%20from%20patients%20with%20autism%20carrying%20de%20novo%20SHANK3%20mutations&rft.jtitle=Scientific%20reports&rft.au=Gouder,%20Laura&rft.date=2019-01-14&rft.volume=9&rft.issue=1&rft.spage=94&rft.epage=94&rft.pages=94-94&rft.artnum=94&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-018-36993-x&rft_dat=%3Cproquest_pubme%3E2179345120%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2167020993&rft_id=info:pmid/30643170&rfr_iscdi=true |