Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high poten...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2018-07, Vol.15 (7), p.535-538
Hauptverfasser:	Vogel, Paul, Moschref, Matin, Li, Qin, Merkle, Tobias, Selvasaravanan, Karthika D., Li, Jin Billy, Stafforst, Thorsten
Format:	Artikel
Sprache:	eng
Schlagworte:	631/45/500 631/80/458/1733 631/80/86 631/92/552 Adenosine Adenosine deaminase Adenosine Deaminase - metabolism Analysis Animals Base Sequence Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brief Communication Cell Line Comparative analysis Editing Guanine - analogs & derivatives Humans Information processing Inosine Life Sciences Molecular chains Organic chemistry Proteomics Ribonucleic acid RNA RNA Editing - physiology RNA processing RNA-Binding Proteins SNARE Proteins - chemistry SNARE Proteins - metabolism Stat1 protein
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!