Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes
and are the main pathogenic species of invasive cryptococcosis among the species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectro...
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| Veröffentlicht in: | Journal of clinical microbiology 2019-01, Vol.57 (1) |
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| creator | Siqueira, Lumena P Machado Gimenes, Viviane M Favero de Freitas, Roseli Santos Melhem, Márcia de Souza Carvalho Bonfietti, Lucas Xavier da Silva, Jr, Afonso Rafael Souza Santos, Letícia B Motta, Adriana L Rossi, Flavia Benard, Gil de Almeida, Jr, João N |
| description | and
are the main pathogenic species of invasive cryptococcosis among the
species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for
and
genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different
(VNI, VNII, VNIII, and VNIV) and
(VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all
and
genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification. |
| doi_str_mv | 10.1128/JCM.01282-18 |
| format | Article |
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are the main pathogenic species of invasive cryptococcosis among the
species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for
and
genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different
(VNI, VNII, VNIII, and VNIV) and
(VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all
and
genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01282-18</identifier><identifier>PMID: 30429250</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Cryptococcosis - microbiology ; Cryptococcus gattii - chemistry ; Cryptococcus gattii - classification ; Cryptococcus gattii - genetics ; Cryptococcus gattii - isolation & purification ; Cryptococcus neoformans - chemistry ; Cryptococcus neoformans - classification ; Cryptococcus neoformans - genetics ; Cryptococcus neoformans - isolation & purification ; Databases, Genetic ; Genotype ; Humans ; Mycological Typing Techniques - methods ; Mycological Typing Techniques - standards ; Mycology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</subject><ispartof>Journal of clinical microbiology, 2019-01, Vol.57 (1)</ispartof><rights>Copyright © 2019 American Society for Microbiology.</rights><rights>Copyright © 2019 American Society for Microbiology. 2019 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-546c15dac491037d97c0c344c320732009d9a122be519daa7b88c7429a8779e43</citedby><cites>FETCH-LOGICAL-c384t-546c15dac491037d97c0c344c320732009d9a122be519daa7b88c7429a8779e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322467/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322467/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30429250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Siqueira, Lumena P Machado</creatorcontrib><creatorcontrib>Gimenes, Viviane M Favero</creatorcontrib><creatorcontrib>de Freitas, Roseli Santos</creatorcontrib><creatorcontrib>Melhem, Márcia de Souza Carvalho</creatorcontrib><creatorcontrib>Bonfietti, Lucas Xavier</creatorcontrib><creatorcontrib>da Silva, Jr, Afonso Rafael</creatorcontrib><creatorcontrib>Souza Santos, Letícia B</creatorcontrib><creatorcontrib>Motta, Adriana L</creatorcontrib><creatorcontrib>Rossi, Flavia</creatorcontrib><creatorcontrib>Benard, Gil</creatorcontrib><creatorcontrib>de Almeida, Jr, João N</creatorcontrib><title>Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>and
are the main pathogenic species of invasive cryptococcosis among the
species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for
and
genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different
(VNI, VNII, VNIII, and VNIV) and
(VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all
and
genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.</description><subject>Cryptococcosis - microbiology</subject><subject>Cryptococcus gattii - chemistry</subject><subject>Cryptococcus gattii - classification</subject><subject>Cryptococcus gattii - genetics</subject><subject>Cryptococcus gattii - isolation & purification</subject><subject>Cryptococcus neoformans - chemistry</subject><subject>Cryptococcus neoformans - classification</subject><subject>Cryptococcus neoformans - genetics</subject><subject>Cryptococcus neoformans - isolation & purification</subject><subject>Databases, Genetic</subject><subject>Genotype</subject><subject>Humans</subject><subject>Mycological Typing Techniques - methods</subject><subject>Mycological Typing Techniques - standards</subject><subject>Mycology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctLAzEQxoMoWqs3z7JHD67mtZvkIkitL1o8-MBbSLPZGt1uapIt9L83Wi32MMzA9-ObGT4AjhA8Qwjz8_vB-AymAeeIb4EegoLnZQlft0EPQlHkCBG2B_ZDeIcQUVoUu2CPQIoFLmAPTIcL1XQqWtdmrs5ebDQf2fgxq53PrmxdG2_aaNf6wC_n0WmndRey1riEzVQbMtVWm9pUxWhtdmNaF5dzEw7ATq2aYA5_ex88Xw-fBrf56OHmbnA5yjXhNOYFLTUqKqWpQJCwSjANNaFUEwxZKigqoRDGE1MgUSnFJpxrlp5RnDFhKOmDi5XvvJvMTKXT9V41cu7tTPmldMrKTaW1b3LqFrIkGNOSJYOTXwPvPjsTopzZoE3TqPRuFyRGhHBMKRIJPV2h2rsQvKnXaxCU39nIlI38yUYinvDj_6et4b8wyBeW0ouD</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Siqueira, Lumena P Machado</creator><creator>Gimenes, Viviane M Favero</creator><creator>de Freitas, Roseli Santos</creator><creator>Melhem, Márcia de Souza Carvalho</creator><creator>Bonfietti, Lucas Xavier</creator><creator>da Silva, Jr, Afonso Rafael</creator><creator>Souza Santos, Letícia B</creator><creator>Motta, Adriana L</creator><creator>Rossi, Flavia</creator><creator>Benard, Gil</creator><creator>de Almeida, Jr, João N</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190101</creationdate><title>Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes</title><author>Siqueira, Lumena P Machado ; Gimenes, Viviane M Favero ; de Freitas, Roseli Santos ; Melhem, Márcia de Souza Carvalho ; Bonfietti, Lucas Xavier ; da Silva, Jr, Afonso Rafael ; Souza Santos, Letícia B ; Motta, Adriana L ; Rossi, Flavia ; Benard, Gil ; de Almeida, Jr, João N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-546c15dac491037d97c0c344c320732009d9a122be519daa7b88c7429a8779e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Cryptococcosis - microbiology</topic><topic>Cryptococcus gattii - chemistry</topic><topic>Cryptococcus gattii - classification</topic><topic>Cryptococcus gattii - genetics</topic><topic>Cryptococcus gattii - isolation & purification</topic><topic>Cryptococcus neoformans - chemistry</topic><topic>Cryptococcus neoformans - classification</topic><topic>Cryptococcus neoformans - genetics</topic><topic>Cryptococcus neoformans - isolation & purification</topic><topic>Databases, Genetic</topic><topic>Genotype</topic><topic>Humans</topic><topic>Mycological Typing Techniques - methods</topic><topic>Mycological Typing Techniques - standards</topic><topic>Mycology</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siqueira, Lumena P Machado</creatorcontrib><creatorcontrib>Gimenes, Viviane M Favero</creatorcontrib><creatorcontrib>de Freitas, Roseli Santos</creatorcontrib><creatorcontrib>Melhem, Márcia de Souza Carvalho</creatorcontrib><creatorcontrib>Bonfietti, Lucas Xavier</creatorcontrib><creatorcontrib>da Silva, Jr, Afonso Rafael</creatorcontrib><creatorcontrib>Souza Santos, Letícia B</creatorcontrib><creatorcontrib>Motta, Adriana L</creatorcontrib><creatorcontrib>Rossi, Flavia</creatorcontrib><creatorcontrib>Benard, Gil</creatorcontrib><creatorcontrib>de Almeida, Jr, João N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siqueira, Lumena P Machado</au><au>Gimenes, Viviane M Favero</au><au>de Freitas, Roseli Santos</au><au>Melhem, Márcia de Souza Carvalho</au><au>Bonfietti, Lucas Xavier</au><au>da Silva, Jr, Afonso Rafael</au><au>Souza Santos, Letícia B</au><au>Motta, Adriana L</au><au>Rossi, Flavia</au><au>Benard, Gil</au><au>de Almeida, Jr, João N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>57</volume><issue>1</issue><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>and
are the main pathogenic species of invasive cryptococcosis among the
species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for
and
genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different
(VNI, VNII, VNIII, and VNIV) and
(VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMérieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all
and
genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>30429250</pmid><doi>10.1128/JCM.01282-18</doi><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Cryptococcosis - microbiology Cryptococcus gattii - chemistry Cryptococcus gattii - classification Cryptococcus gattii - genetics Cryptococcus gattii - isolation & purification Cryptococcus neoformans - chemistry Cryptococcus neoformans - classification Cryptococcus neoformans - genetics Cryptococcus neoformans - isolation & purification Databases, Genetic Genotype Humans Mycological Typing Techniques - methods Mycological Typing Techniques - standards Mycology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - standards |
| title | Evaluation of Vitek MS for Differentiation of Cryptococcus neoformans and Cryptococcus gattii Genotypes |
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