Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time

Real-time, isothermal, digital nucleic acid amplification is emerging as an attractive approach for a multitude of applications including diagnostics, mechanistic studies, and assay optimization. Unfortunately, there is no commercially available and affordable real-time, digital instrument validated...

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Veröffentlicht in:	Analytical chemistry (Washington) 2019-01, Vol.91 (1), p.1034-1042
Hauptverfasser:	Rolando, Justin C, Jue, Erik, Schoepp, Nathan G, Ismagilov, Rustem F
Format:	Artikel
Sprache:	eng
Schlagworte:	Allelopathy Analytical chemistry Anti-Bacterial Agents - urine antibiotic resistance Antibiotics Assaying Biochemistry Capillary electrophoresis Chemistry diagnostic techniques Diagnostic Tests, Routine DNA, Bacterial - isolation & purification DNA, Bacterial - urine Efficiency enzymes Escherichia coli - isolation & purification Fluids gene amplification Humans Kinetics Laboratory equipment loop-mediated isothermal amplification Microfluidic Analytical Techniques - instrumentation Microfluidics Nucleic Acid Amplification Techniques - instrumentation Nucleic acids Optimization Organic chemistry patients Phenotype probability Reagents Real time Sensitivity and Specificity Temperature Test procedures Time Factors Urinary tract urinary tract diseases Urine
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