Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize

Summary CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9‐...

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Veröffentlicht in:	Plant biotechnology journal 2019-02, Vol.17 (2), p.362-372
Hauptverfasser:	Lee, Keunsub, Zhang, Yingxiao, Kleinstiver, Benjamin P., Guo, Jimmy A., Aryee, Martin J., Miller, Jonah, Malzahn, Aimee, Zarecor, Scott, Lawrence‐Dill, Carolyn J., Joung, J. Keith, Qi, Yiping, Wang, Kan
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium biotechnology Cas12a (Cpf1) CIRCLE‐seq Comparative analysis Corn CRISPR CRISPR-Cas Systems CRISPR/Cas DNA binding proteins Editing Embryos Endonucleases - genetics Endonucleases - metabolism Gene Editing - methods Gene sequencing Gene Targeting - methods genes Genetic transformation genome editing Genome, Plant - genetics Genomes Genomics gRNA homozygosity Inbreeding Mutagenesis Mutation mutational analysis Nuclease Nucleases off‐target Plants Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics Sequence Alignment Site-directed mutagenesis Target recognition Zea mays Zea mays - enzymology Zea mays - genetics
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