HIPMap: A High-Throughput Imaging Method for Mapping Spatial Gene Positions
The three-dimensional organization of genes inside the cell nucleus affects their functions including DNA transcription, replication, and repair. A major goal in the field of nuclear architecture is to determine what cellular factors establish and maintain the position of individual genes. Here, we...
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| Veröffentlicht in: | Cold Spring Harbor Symposia on Quantitative Biology 2015-01, Vol.80, p.73-81 |
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| creator | Shachar, Sigal Pegoraro, Gianluca Misteli, Tom |
| description | The three-dimensional organization of genes inside the cell nucleus affects their functions including DNA transcription, replication, and repair. A major goal in the field of nuclear architecture is to determine what cellular factors establish and maintain the position of individual genes. Here, we describe HIPMap, a high-throughput imaging and analysis pipeline for the mapping of endogenous gene loci within the 3D space of the nucleus. HIPMap can be used for a variety of applications including screening, mapping translocations, validating chromosome conformation capture data, probing DNA-protein interactions, and interrogation of the relationship of gene expression with localization. |
| doi_str_mv | 10.1101/sqb.2015.80.027417 |
| format | Article |
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| subjects | Animals Cell Nucleus Chromosome Mapping Chromosomes DNA Gene Expression Regulation Humans Imaging, Three-Dimensional In Situ Hybridization, Fluorescence |
| title | HIPMap: A High-Throughput Imaging Method for Mapping Spatial Gene Positions |
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