Salivary protease spectrum biomarkers of oral cancer

Proteases are important molecules that are involved in many physiological and pathological processes of the human body, such as growth, apoptosis and metastasis cancer cells. They are potential targets in cancer diagnosis and biotherapy. In this study, we analyzed the salivary protease spectrum of patients with oral squamous cell carcinoma (OSCC), oral benign masses and chronic periodontitis, as well as that of health, using human protease array kits, enzyme-linked immunosorbent assay, western blot and immunofluorescence. The salivary protease spectrum was found to be associated with oral diseases. For example, the saliva of patients with OSCC contained increased numbers of proteases than those of other oral diseases and health. The levels of matrix metalloproteinase (MMP)-1, MMP-2, MMP-10, MMP-12, A disintegrin and metalloprotease (ADAM)9, A disintegrin and metalloprotease with thrombospondin type 13 motifs (ADAMST13), cathepsin V and kallikrein 5 in the saliva of patients with OSCC were significantly increased compared with those of other groups. Taking MMP-1, cathepsin V, kallikrein 5 and ADAM9 as biomarkers of OSCC, cutoff values were199, 11.34, 9.29 and 202.55 pg·mL −1 , respectively. From the area under the curve, sensitivity and specificity, the combination of cathepsin V/kallikrein5/ADAM9 was an optimal biomarker for diagnosing OSCC. Thus, analysis of the salivary protease spectrum may be an innovative and cost-efficient approach to evaluating the health status of the oral cavity. Specifically, increases in cathepsin V, kallikrein 5 and ADAM9 may be useful biomarkers in the screening and diagnosis of OSCC. Cancer: Salivary enzymes show cancer signature Patients with oral cancer have elevated levels of certain protease enzymes in their saliva, a finding that could help with screening and diagnosis. Xuedong Zhou and colleagues from Sichuan University, China, collected saliva samples from patients with different mouth diseases—a type of cancer known as oral squamous cell carcinoma, benign mouth lesions or mild chronic gum disease—as well as healthy controls. They tested the saliva for levels of 35 different proteases, a class of enzymes involved in cleaving proteins, and identified several proteases present only in the saliva of patients with oral cancer, three of which they showed were expressed at high levels in cultured oral tumor cell lines as well. These proteases could thus serve as useful biomarkers in a future spit test for early cancer detect