Postmortem distribution of mepirapim and acetyl fentanyl in biological fluid and solid tissue specimens measured by the standard addition method

Purpose Mepirapim is a new synthetic cannabinoid. We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased indi...

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Veröffentlicht in:Forensic toxicology 2019, Vol.37 (1), p.27-33
Hauptverfasser: Mochizuki, Akira, Nakazawa, Hiroko, Adachi, Noboru, Takekawa, Kenichi, Shojo, Hideki
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container_end_page 33
container_issue 1
container_start_page 27
container_title Forensic toxicology
container_volume 37
creator Mochizuki, Akira
Nakazawa, Hiroko
Adachi, Noboru
Takekawa, Kenichi
Shojo, Hideki
description Purpose Mepirapim is a new synthetic cannabinoid. We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased individual, we established a standard addition method for detailed analysis by liquid chromatography–mass spectrometry (LC–MS) for quantification of these drugs. Methods The LC–MS method was fully validated for linearity, extraction recovery, matrix effect and repeatability. Results Good linearities, extraction recoveries, matrix effects and repeatabilities were shown for both target compounds in all specimens. The concentrations of mepirapim and acetyl fentanyl in three body fluid specimens and 12 solid tissue specimens were measured. For mepirapim, the highest concentrations were found in the liver and kidney, and the concentrations in the blood and urine specimens were one order of magnitude lower than the high concentrations in the solid tissues except the psoas major muscle. For acetyl fentanyl, the highest concentrations were found in the myocardium, spleen and kidney, and the concentrations in the body fluid specimens were also one order of magnitude lower than the highest concentrations in the solid tissues. There were concentration differences of mepirapim and acetyl fentanyl among the regions of the brain. Conclusions The concentration of unchanged mepirapim in urine was much higher than those of other synthetic cannabinoids; the higher dosage, urinary excretion, metabolisms and/or pharmacokinetics of mepirapim may be quite different from those of other synthetic cannabinoids.
doi_str_mv 10.1007/s11419-018-0431-z
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We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased individual, we established a standard addition method for detailed analysis by liquid chromatography–mass spectrometry (LC–MS) for quantification of these drugs. Methods The LC–MS method was fully validated for linearity, extraction recovery, matrix effect and repeatability. Results Good linearities, extraction recoveries, matrix effects and repeatabilities were shown for both target compounds in all specimens. The concentrations of mepirapim and acetyl fentanyl in three body fluid specimens and 12 solid tissue specimens were measured. For mepirapim, the highest concentrations were found in the liver and kidney, and the concentrations in the blood and urine specimens were one order of magnitude lower than the high concentrations in the solid tissues except the psoas major muscle. For acetyl fentanyl, the highest concentrations were found in the myocardium, spleen and kidney, and the concentrations in the body fluid specimens were also one order of magnitude lower than the highest concentrations in the solid tissues. There were concentration differences of mepirapim and acetyl fentanyl among the regions of the brain. Conclusions The concentration of unchanged mepirapim in urine was much higher than those of other synthetic cannabinoids; the higher dosage, urinary excretion, metabolisms and/or pharmacokinetics of mepirapim may be quite different from those of other synthetic cannabinoids.</description><identifier>ISSN: 1860-8965</identifier><identifier>EISSN: 1860-8973</identifier><identifier>DOI: 10.1007/s11419-018-0431-z</identifier><identifier>PMID: 30636981</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Forensic Medicine ; Forensic Science ; Medical Law ; Medicinal Chemistry ; Medicine ; Medicine &amp; Public Health ; Original ; Original Article ; Pharmacology/Toxicology</subject><ispartof>Forensic toxicology, 2019, Vol.37 (1), p.27-33</ispartof><rights>The Author(s) 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447z-34d9f61313e4f525474a47d2e0209d0426296f8df5b15776d21ca2d1f9cd19d03</citedby><cites>FETCH-LOGICAL-c447z-34d9f61313e4f525474a47d2e0209d0426296f8df5b15776d21ca2d1f9cd19d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11419-018-0431-z$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11419-018-0431-z$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,4025,27928,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30636981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mochizuki, Akira</creatorcontrib><creatorcontrib>Nakazawa, Hiroko</creatorcontrib><creatorcontrib>Adachi, Noboru</creatorcontrib><creatorcontrib>Takekawa, Kenichi</creatorcontrib><creatorcontrib>Shojo, Hideki</creatorcontrib><title>Postmortem distribution of mepirapim and acetyl fentanyl in biological fluid and solid tissue specimens measured by the standard addition method</title><title>Forensic toxicology</title><addtitle>Forensic Toxicol</addtitle><addtitle>Forensic Toxicol</addtitle><description>Purpose Mepirapim is a new synthetic cannabinoid. We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased individual, we established a standard addition method for detailed analysis by liquid chromatography–mass spectrometry (LC–MS) for quantification of these drugs. Methods The LC–MS method was fully validated for linearity, extraction recovery, matrix effect and repeatability. Results Good linearities, extraction recoveries, matrix effects and repeatabilities were shown for both target compounds in all specimens. The concentrations of mepirapim and acetyl fentanyl in three body fluid specimens and 12 solid tissue specimens were measured. For mepirapim, the highest concentrations were found in the liver and kidney, and the concentrations in the blood and urine specimens were one order of magnitude lower than the high concentrations in the solid tissues except the psoas major muscle. For acetyl fentanyl, the highest concentrations were found in the myocardium, spleen and kidney, and the concentrations in the body fluid specimens were also one order of magnitude lower than the highest concentrations in the solid tissues. There were concentration differences of mepirapim and acetyl fentanyl among the regions of the brain. 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We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased individual, we established a standard addition method for detailed analysis by liquid chromatography–mass spectrometry (LC–MS) for quantification of these drugs. Methods The LC–MS method was fully validated for linearity, extraction recovery, matrix effect and repeatability. Results Good linearities, extraction recoveries, matrix effects and repeatabilities were shown for both target compounds in all specimens. The concentrations of mepirapim and acetyl fentanyl in three body fluid specimens and 12 solid tissue specimens were measured. For mepirapim, the highest concentrations were found in the liver and kidney, and the concentrations in the blood and urine specimens were one order of magnitude lower than the high concentrations in the solid tissues except the psoas major muscle. For acetyl fentanyl, the highest concentrations were found in the myocardium, spleen and kidney, and the concentrations in the body fluid specimens were also one order of magnitude lower than the highest concentrations in the solid tissues. There were concentration differences of mepirapim and acetyl fentanyl among the regions of the brain. Conclusions The concentration of unchanged mepirapim in urine was much higher than those of other synthetic cannabinoids; the higher dosage, urinary excretion, metabolisms and/or pharmacokinetics of mepirapim may be quite different from those of other synthetic cannabinoids.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>30636981</pmid><doi>10.1007/s11419-018-0431-z</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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subjects Forensic Medicine
Forensic Science
Medical Law
Medicinal Chemistry
Medicine
Medicine & Public Health
Original
Original Article
Pharmacology/Toxicology
title Postmortem distribution of mepirapim and acetyl fentanyl in biological fluid and solid tissue specimens measured by the standard addition method
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