Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors
Eight yeast isolates identified as Saccharomyces cerevisiae were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79...
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| Veröffentlicht in: | 3 Biotech 2019, Vol.9 (1), p.6-6, Article 6 |
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| creator | Cabañas, Keyla Tortoló Peña-Moreno, Irina Charlot Parente, Denise Castro García, Antonio Bell Gutiérrez, Roxana García de Morais Jr, Marcos Antonio |
| description | Eight yeast isolates identified as
Saccharomyces cerevisiae
were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79 showed high ethanol yield from sugarcane molasses. The isolate L/25-7-86 also displayed high fermentation capacity when molasses was diluted with vinasse. In addition, stress tolerance was evaluated on the basis of growth in the presence of inhibitors (acetic acid, lactic acid, 5-hydroxymethylfurfural and sulfuric acid) and the results indicated that L/25-7-77 and L/25-7-79 congregated the highest score for cross-tolerance and fermentation capacity. Hence, these isolates, especially L/25-7-77, could serve as potential biological platform for the arduous task of fermenting complex substrates that contain inhibitors. The use of these yeasts was discussed in the context of second-generation ethanol and the environmental and economic implications of the use of vinasse, saving the use of water for substrate dilution. |
| doi_str_mv | 10.1007/s13205-018-1541-3 |
| format | Article |
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Saccharomyces cerevisiae
were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79 showed high ethanol yield from sugarcane molasses. The isolate L/25-7-86 also displayed high fermentation capacity when molasses was diluted with vinasse. In addition, stress tolerance was evaluated on the basis of growth in the presence of inhibitors (acetic acid, lactic acid, 5-hydroxymethylfurfural and sulfuric acid) and the results indicated that L/25-7-77 and L/25-7-79 congregated the highest score for cross-tolerance and fermentation capacity. Hence, these isolates, especially L/25-7-77, could serve as potential biological platform for the arduous task of fermenting complex substrates that contain inhibitors. The use of these yeasts was discussed in the context of second-generation ethanol and the environmental and economic implications of the use of vinasse, saving the use of water for substrate dilution.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-018-1541-3</identifier><identifier>PMID: 30622844</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Acetic acid ; Agriculture ; Baking yeast ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Chemistry ; Chemistry and Materials Science ; Cross-tolerance ; Dilution ; Distilleries ; Economic conditions ; Ethanol ; ethanol production ; Fermentation ; Hydroxymethylfurfural ; Inhibitors ; internal transcribed spacers ; Lactic acid ; loci ; Molasses ; Nucleotide sequence ; Original ; Original Article ; Saccharomyces cerevisiae ; sequence analysis ; Stem Cells ; stress tolerance ; Substrate inhibition ; Sugarcane ; sugarcane juice ; Sulfuric acid ; Vinasse ; Water conservation ; Yeast ; yeasts</subject><ispartof>3 Biotech, 2019, Vol.9 (1), p.6-6, Article 6</ispartof><rights>King Abdulaziz City for Science and Technology 2019</rights><rights>3 Biotech is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-48123d7b1f944538962f708bbc2b6b9f875b5418dda9231d8d520842fd59fdef3</citedby><cites>FETCH-LOGICAL-c503t-48123d7b1f944538962f708bbc2b6b9f875b5418dda9231d8d520842fd59fdef3</cites><orcidid>0000-0002-9727-0300</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312825/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312825/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30622844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cabañas, Keyla Tortoló</creatorcontrib><creatorcontrib>Peña-Moreno, Irina Charlot</creatorcontrib><creatorcontrib>Parente, Denise Castro</creatorcontrib><creatorcontrib>García, Antonio Bell</creatorcontrib><creatorcontrib>Gutiérrez, Roxana García</creatorcontrib><creatorcontrib>de Morais Jr, Marcos Antonio</creatorcontrib><title>Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>Eight yeast isolates identified as
Saccharomyces cerevisiae
were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79 showed high ethanol yield from sugarcane molasses. The isolate L/25-7-86 also displayed high fermentation capacity when molasses was diluted with vinasse. In addition, stress tolerance was evaluated on the basis of growth in the presence of inhibitors (acetic acid, lactic acid, 5-hydroxymethylfurfural and sulfuric acid) and the results indicated that L/25-7-77 and L/25-7-79 congregated the highest score for cross-tolerance and fermentation capacity. Hence, these isolates, especially L/25-7-77, could serve as potential biological platform for the arduous task of fermenting complex substrates that contain inhibitors. The use of these yeasts was discussed in the context of second-generation ethanol and the environmental and economic implications of the use of vinasse, saving the use of water for substrate dilution.</description><subject>Acetic acid</subject><subject>Agriculture</subject><subject>Baking yeast</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cross-tolerance</subject><subject>Dilution</subject><subject>Distilleries</subject><subject>Economic conditions</subject><subject>Ethanol</subject><subject>ethanol production</subject><subject>Fermentation</subject><subject>Hydroxymethylfurfural</subject><subject>Inhibitors</subject><subject>internal transcribed spacers</subject><subject>Lactic acid</subject><subject>loci</subject><subject>Molasses</subject><subject>Nucleotide sequence</subject><subject>Original</subject><subject>Original Article</subject><subject>Saccharomyces cerevisiae</subject><subject>sequence analysis</subject><subject>Stem Cells</subject><subject>stress tolerance</subject><subject>Substrate inhibition</subject><subject>Sugarcane</subject><subject>sugarcane juice</subject><subject>Sulfuric acid</subject><subject>Vinasse</subject><subject>Water conservation</subject><subject>Yeast</subject><subject>yeasts</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFkctq3TAQhk1paUKSB-imGLrpxq00six5UyghvUCgizSQnZClUazgI51KdiBvXxmnpxco0UZi5pt_ZvRX1StK3lFCxPtMGRDeECobylvasGfVMdCeNFww-fzwhpuj6iznO1IOp7yn5GV1xEgHINv2uDJXOKGZfQx1dPWVNmbUKe4eDObaYMJ7n73G2uc46bnEXEw1zqMOcar3Kdplq_WhnkcsEcwYDK5aPox-8HNM-bR64fSU8ezxPqmuP118P__SXH77_PX842VjOGFz00oKzIqBur5tOZN9B04QOQwGhm7onRR8KItKa3UPjFppORDZgrO8dxYdO6k-bLr7ZdihNRjmpCe1T36n04OK2qu_M8GP6jbeq45RkMCLwNtHgRR_LJhntfPZ4DTpgHHJCgBITwgV8mmUdrzrOkFZQd_8g97FJYXyEysFTAgQa2-6USbFnBO6w9yUqNVwtRmuiuFqNVytyq__XPhQ8cveAsAG5JIKt5h-t_6_6k9cO7av</recordid><startdate>2019</startdate><enddate>2019</enddate><creator>Cabañas, Keyla Tortoló</creator><creator>Peña-Moreno, Irina Charlot</creator><creator>Parente, Denise Castro</creator><creator>García, Antonio Bell</creator><creator>Gutiérrez, Roxana García</creator><creator>de Morais Jr, Marcos Antonio</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9727-0300</orcidid></search><sort><creationdate>2019</creationdate><title>Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors</title><author>Cabañas, Keyla Tortoló ; Peña-Moreno, Irina Charlot ; Parente, Denise Castro ; García, Antonio Bell ; Gutiérrez, Roxana García ; de Morais Jr, Marcos Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-48123d7b1f944538962f708bbc2b6b9f875b5418dda9231d8d520842fd59fdef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetic acid</topic><topic>Agriculture</topic><topic>Baking yeast</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cross-tolerance</topic><topic>Dilution</topic><topic>Distilleries</topic><topic>Economic conditions</topic><topic>Ethanol</topic><topic>ethanol production</topic><topic>Fermentation</topic><topic>Hydroxymethylfurfural</topic><topic>Inhibitors</topic><topic>internal transcribed spacers</topic><topic>Lactic acid</topic><topic>loci</topic><topic>Molasses</topic><topic>Nucleotide sequence</topic><topic>Original</topic><topic>Original Article</topic><topic>Saccharomyces cerevisiae</topic><topic>sequence analysis</topic><topic>Stem Cells</topic><topic>stress tolerance</topic><topic>Substrate inhibition</topic><topic>Sugarcane</topic><topic>sugarcane juice</topic><topic>Sulfuric acid</topic><topic>Vinasse</topic><topic>Water conservation</topic><topic>Yeast</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cabañas, Keyla Tortoló</creatorcontrib><creatorcontrib>Peña-Moreno, Irina Charlot</creatorcontrib><creatorcontrib>Parente, Denise Castro</creatorcontrib><creatorcontrib>García, Antonio Bell</creatorcontrib><creatorcontrib>Gutiérrez, Roxana García</creatorcontrib><creatorcontrib>de Morais Jr, Marcos Antonio</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cabañas, Keyla Tortoló</au><au>Peña-Moreno, Irina Charlot</au><au>Parente, Denise Castro</au><au>García, Antonio Bell</au><au>Gutiérrez, Roxana García</au><au>de Morais Jr, Marcos Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2019</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>6</spage><epage>6</epage><pages>6-6</pages><artnum>6</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>Eight yeast isolates identified as
Saccharomyces cerevisiae
were recovered from molasses-using Cuban distilleries and discriminated by nucleotide sequence analysis of ITS locus. The isolates L/25-7-81 and L/25-7-86 showed the highest ethanol yield from sugarcane juice, while L/25-7-12 and L/25-7-79 showed high ethanol yield from sugarcane molasses. The isolate L/25-7-86 also displayed high fermentation capacity when molasses was diluted with vinasse. In addition, stress tolerance was evaluated on the basis of growth in the presence of inhibitors (acetic acid, lactic acid, 5-hydroxymethylfurfural and sulfuric acid) and the results indicated that L/25-7-77 and L/25-7-79 congregated the highest score for cross-tolerance and fermentation capacity. Hence, these isolates, especially L/25-7-77, could serve as potential biological platform for the arduous task of fermenting complex substrates that contain inhibitors. The use of these yeasts was discussed in the context of second-generation ethanol and the environmental and economic implications of the use of vinasse, saving the use of water for substrate dilution.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>30622844</pmid><doi>10.1007/s13205-018-1541-3</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9727-0300</orcidid><oa>free_for_read</oa></addata></record> |
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| source | SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acetic acid Agriculture Baking yeast Bioinformatics Biomaterials Biotechnology Cancer Research Chemistry Chemistry and Materials Science Cross-tolerance Dilution Distilleries Economic conditions Ethanol ethanol production Fermentation Hydroxymethylfurfural Inhibitors internal transcribed spacers Lactic acid loci Molasses Nucleotide sequence Original Original Article Saccharomyces cerevisiae sequence analysis Stem Cells stress tolerance Substrate inhibition Sugarcane sugarcane juice Sulfuric acid Vinasse Water conservation Yeast yeasts |
| title | Selection of Saccharomyces cerevisiae isolates for ethanol production in the presence of inhibitors |
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