microRNA-200a functions as a tumor suppressor by targeting FOXA1 in glioma
microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR-200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR-200a in glioma as well as the regulatory mechanism of miR-200a in gl...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2019-01, Vol.17 (1), p.221-229 |
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| creator | Chen, Xiaofeng Liu, Kun Yang, Ping Kuang, Weiping Huang, Hongxing Tu, Ewen Li, Bo Zhu, Yong Zhou, Bin Yan, Lin |
| description | microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR-200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR-200a in glioma as well as the regulatory mechanism of miR-200a in glioma cell proliferation, survival and invasion. RT-qPCR and western blotting were performed to examine mRNA and protein expression. An MTT assay, an EdU incorporation cell proliferation assay and a transwell assay were utilized to assess cell survival, proliferation and invasion. The results indicated that the miR-200a levels were significantly reduced in glioma tissues compared with normal brain tissues. Levels were also downregulated in glioma cell lines when compared with those in normal human astrocyte cells. Furthermore, low miR-200a expression was associated with advanced progression of glioma. The overexpression of miR-200a inhibited glioma cell proliferation, survival and invasion. Results also identified that FOXA1 was a target gene of miR-200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR-200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR-200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR-200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR-200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma. |
| doi_str_mv | 10.3892/etm.2018.6895 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6307448</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A571367765</galeid><sourcerecordid>A571367765</sourcerecordid><originalsourceid>FETCH-LOGICAL-c552t-46eac3b947b738120c8fa4b0e28b4300e452c6941059228f78039b08419510a73</originalsourceid><addsrcrecordid>eNptkc9rFTEQxxdRbKk9epUFL172OfmdXIRHsbZSLIiCt5BNs2vKbvJMdoX-986jz2rFJJAh-cx3mPk2zUsCG6YNfRuWeUOB6I3URjxpjokytCNAxNNDDEaTo-a01lvAJSTRWjxvjhhIQZSWx83HOfqSP3_adhTAtcOa_BJzqq3D0y7rnEtb192uhFox7O_axZUxLDGN7fn1ty1pY2rHKebZvWieDW6q4fRwnzRfz99_Obvorq4_XJ5trzovBF06LoPzrDdc9YppQsHrwfEeAtU9ZwCBC-ql4QSEoVQPSgMzPWhOjCDgFDtp3t3r7tZ-Djc-pKW4ye5KnF25s9lF-_gnxe92zD-tZKA41yjw5iBQ8o811MXOsfowTS6FvFZLcXQMywqO6Ot_0Nu8loTtISWpQkEBf6jRTcHGNGSs6_eidisUYVIpKZDa_IfCfRPQhJzCEPH9UUJ3n4AO1VrC8NAjAbv336L_du-_3fuP_Ku_B_NA_3ab_QJa-qcw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2162707450</pqid></control><display><type>article</type><title>microRNA-200a functions as a tumor suppressor by targeting FOXA1 in glioma</title><source>PubMed Central</source><creator>Chen, Xiaofeng ; Liu, Kun ; Yang, Ping ; Kuang, Weiping ; Huang, Hongxing ; Tu, Ewen ; Li, Bo ; Zhu, Yong ; Zhou, Bin ; Yan, Lin</creator><creatorcontrib>Chen, Xiaofeng ; Liu, Kun ; Yang, Ping ; Kuang, Weiping ; Huang, Hongxing ; Tu, Ewen ; Li, Bo ; Zhu, Yong ; Zhou, Bin ; Yan, Lin</creatorcontrib><description>microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR-200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR-200a in glioma as well as the regulatory mechanism of miR-200a in glioma cell proliferation, survival and invasion. RT-qPCR and western blotting were performed to examine mRNA and protein expression. An MTT assay, an EdU incorporation cell proliferation assay and a transwell assay were utilized to assess cell survival, proliferation and invasion. The results indicated that the miR-200a levels were significantly reduced in glioma tissues compared with normal brain tissues. Levels were also downregulated in glioma cell lines when compared with those in normal human astrocyte cells. Furthermore, low miR-200a expression was associated with advanced progression of glioma. The overexpression of miR-200a inhibited glioma cell proliferation, survival and invasion. Results also identified that FOXA1 was a target gene of miR-200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR-200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR-200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR-200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR-200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2018.6895</identifier><identifier>PMID: 30651786</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Age ; Apoptosis ; Brain cancer ; Breast cancer ; Cell growth ; Development and progression ; Esophageal cancer ; Gastric cancer ; Gene expression ; Genetic aspects ; Glioma ; Gliomas ; Hospitals ; Liver cancer ; Medical prognosis ; Metastasis ; MicroRNA ; MicroRNAs ; Motility ; Prognosis ; Roles ; Transcription factors</subject><ispartof>Experimental and therapeutic medicine, 2019-01, Vol.17 (1), p.221-229</ispartof><rights>COPYRIGHT 2019 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2019</rights><rights>Copyright: © Chen et al. 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-46eac3b947b738120c8fa4b0e28b4300e452c6941059228f78039b08419510a73</citedby><cites>FETCH-LOGICAL-c552t-46eac3b947b738120c8fa4b0e28b4300e452c6941059228f78039b08419510a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307448/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307448/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30651786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xiaofeng</creatorcontrib><creatorcontrib>Liu, Kun</creatorcontrib><creatorcontrib>Yang, Ping</creatorcontrib><creatorcontrib>Kuang, Weiping</creatorcontrib><creatorcontrib>Huang, Hongxing</creatorcontrib><creatorcontrib>Tu, Ewen</creatorcontrib><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Zhu, Yong</creatorcontrib><creatorcontrib>Zhou, Bin</creatorcontrib><creatorcontrib>Yan, Lin</creatorcontrib><title>microRNA-200a functions as a tumor suppressor by targeting FOXA1 in glioma</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR-200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR-200a in glioma as well as the regulatory mechanism of miR-200a in glioma cell proliferation, survival and invasion. RT-qPCR and western blotting were performed to examine mRNA and protein expression. An MTT assay, an EdU incorporation cell proliferation assay and a transwell assay were utilized to assess cell survival, proliferation and invasion. The results indicated that the miR-200a levels were significantly reduced in glioma tissues compared with normal brain tissues. Levels were also downregulated in glioma cell lines when compared with those in normal human astrocyte cells. Furthermore, low miR-200a expression was associated with advanced progression of glioma. The overexpression of miR-200a inhibited glioma cell proliferation, survival and invasion. Results also identified that FOXA1 was a target gene of miR-200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR-200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR-200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR-200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR-200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma.</description><subject>Age</subject><subject>Apoptosis</subject><subject>Brain cancer</subject><subject>Breast cancer</subject><subject>Cell growth</subject><subject>Development and progression</subject><subject>Esophageal cancer</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Glioma</subject><subject>Gliomas</subject><subject>Hospitals</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>Motility</subject><subject>Prognosis</subject><subject>Roles</subject><subject>Transcription factors</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkc9rFTEQxxdRbKk9epUFL172OfmdXIRHsbZSLIiCt5BNs2vKbvJMdoX-986jz2rFJJAh-cx3mPk2zUsCG6YNfRuWeUOB6I3URjxpjokytCNAxNNDDEaTo-a01lvAJSTRWjxvjhhIQZSWx83HOfqSP3_adhTAtcOa_BJzqq3D0y7rnEtb192uhFox7O_axZUxLDGN7fn1ty1pY2rHKebZvWieDW6q4fRwnzRfz99_Obvorq4_XJ5trzovBF06LoPzrDdc9YppQsHrwfEeAtU9ZwCBC-ql4QSEoVQPSgMzPWhOjCDgFDtp3t3r7tZ-Djc-pKW4ye5KnF25s9lF-_gnxe92zD-tZKA41yjw5iBQ8o811MXOsfowTS6FvFZLcXQMywqO6Ot_0Nu8loTtISWpQkEBf6jRTcHGNGSs6_eidisUYVIpKZDa_IfCfRPQhJzCEPH9UUJ3n4AO1VrC8NAjAbv336L_du-_3fuP_Ku_B_NA_3ab_QJa-qcw</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Chen, Xiaofeng</creator><creator>Liu, Kun</creator><creator>Yang, Ping</creator><creator>Kuang, Weiping</creator><creator>Huang, Hongxing</creator><creator>Tu, Ewen</creator><creator>Li, Bo</creator><creator>Zhu, Yong</creator><creator>Zhou, Bin</creator><creator>Yan, Lin</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. 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Results also identified that FOXA1 was a target gene of miR-200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR-200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR-200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR-200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR-200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30651786</pmid><doi>10.3892/etm.2018.6895</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age Apoptosis Brain cancer Breast cancer Cell growth Development and progression Esophageal cancer Gastric cancer Gene expression Genetic aspects Glioma Gliomas Hospitals Liver cancer Medical prognosis Metastasis MicroRNA MicroRNAs Motility Prognosis Roles Transcription factors |
| title | microRNA-200a functions as a tumor suppressor by targeting FOXA1 in glioma |
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