Morphine and ketamine treatment suppress the differentiation of T helper cells of patients with colorectal cancer in vitro
There have been conflicting reports regarding the effects of anesthetic and analgesic drugs on immune function in patients with cancer. The aim of the present study was to investigate changes to T helper (Th) cell populations in patients with colorectal cancer (CRC) and to assess the effects of morp...
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Veröffentlicht in: | Experimental and therapeutic medicine 2019-01, Vol.17 (1), p.935-942 |
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description | There have been conflicting reports regarding the effects of anesthetic and analgesic drugs on immune function in patients with cancer. The aim of the present study was to investigate changes to T helper (Th) cell populations in patients with colorectal cancer (CRC) and to assess the effects of morphine and ketamine on the differentiation of Th cells harvested from patients with CRC
. Peripheral blood samples were extracted from 20 patients with CRC and 20 healthy participants. Peripheral blood mononuclear cells were isolated and incubated in a solution containing phorbol-myristate-acetate (PMA) and ionomycin in the presence or absence of morphine or various ketamine concentrations (25, 50, and 100 µM). Samples were analyzed 4 h later. Th1 and Th2 cells were significantly increased by PMA and ionomycin stimulation; however, Th1 cells and the Th1/Th2 ratio in PMA and ionomycin treatments were significantly decreased in the CRC group compared with the control group. Following incubation with PMA and ionomycin, morphine significantly decreased Th1 cells and the Th1/Th2 ratio in the CRC group. Ketamine did not significantly affect levels of Th1 or Th2 cells or the Th1/Th2 ratio at a concentration of 25 µM; however, a significant increase in the Th1/Th2 ratio was observed at a concentration of 50 µM and, at 100 µM, a significant decrease in Th1 and Th2 cells and an increase in the Th1/Th2 ratio were observed. The present study suggests that CRC may shift the balance of Th1/Th2 towards Th2 by inducing an immunological response, morphine is able to suppress the differentiation of Th cells and decreases the Th1/Th2 ratio, and ketamine may affect the differentiation of Th cells in a dose-dependent manner. |
doi_str_mv | 10.3892/etm.2018.7035 |
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. Peripheral blood samples were extracted from 20 patients with CRC and 20 healthy participants. Peripheral blood mononuclear cells were isolated and incubated in a solution containing phorbol-myristate-acetate (PMA) and ionomycin in the presence or absence of morphine or various ketamine concentrations (25, 50, and 100 µM). Samples were analyzed 4 h later. Th1 and Th2 cells were significantly increased by PMA and ionomycin stimulation; however, Th1 cells and the Th1/Th2 ratio in PMA and ionomycin treatments were significantly decreased in the CRC group compared with the control group. Following incubation with PMA and ionomycin, morphine significantly decreased Th1 cells and the Th1/Th2 ratio in the CRC group. Ketamine did not significantly affect levels of Th1 or Th2 cells or the Th1/Th2 ratio at a concentration of 25 µM; however, a significant increase in the Th1/Th2 ratio was observed at a concentration of 50 µM and, at 100 µM, a significant decrease in Th1 and Th2 cells and an increase in the Th1/Th2 ratio were observed. The present study suggests that CRC may shift the balance of Th1/Th2 towards Th2 by inducing an immunological response, morphine is able to suppress the differentiation of Th cells and decreases the Th1/Th2 ratio, and ketamine may affect the differentiation of Th cells in a dose-dependent manner.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2018.7035</identifier><identifier>PMID: 30651883</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Analgesics ; Antigens ; Apoptosis ; Care and treatment ; Cell differentiation ; Chemotherapy ; Clinical medicine ; Colorectal cancer ; Cytokines ; Dosage and administration ; Drug therapy ; Immunology ; Ketamine ; Lymphocytes ; Morphine ; Mortality ; Narcotics ; Pain ; Patient outcomes ; Radiation therapy ; Studies ; Substance abuse treatment ; T cells ; Tumors</subject><ispartof>Experimental and therapeutic medicine, 2019-01, Vol.17 (1), p.935-942</ispartof><rights>COPYRIGHT 2019 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2019</rights><rights>Copyright © 2019, Spandidos Publications 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c578t-fcbdc1c3de03e458f350b7afde8e0c07d6e001703a6610e4e8ad5502986465633</citedby><cites>FETCH-LOGICAL-c578t-fcbdc1c3de03e458f350b7afde8e0c07d6e001703a6610e4e8ad5502986465633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307359/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307359/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30651883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Min</creatorcontrib><creatorcontrib>Zhou, Naibao</creatorcontrib><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Wang, Baosheng</creatorcontrib><creatorcontrib>Wang, Xiuqin</creatorcontrib><creatorcontrib>Wang, Xingwu</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Wang, Kaiguo</creatorcontrib><creatorcontrib>Xue, Fushan</creatorcontrib><title>Morphine and ketamine treatment suppress the differentiation of T helper cells of patients with colorectal cancer in vitro</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>There have been conflicting reports regarding the effects of anesthetic and analgesic drugs on immune function in patients with cancer. The aim of the present study was to investigate changes to T helper (Th) cell populations in patients with colorectal cancer (CRC) and to assess the effects of morphine and ketamine on the differentiation of Th cells harvested from patients with CRC
. Peripheral blood samples were extracted from 20 patients with CRC and 20 healthy participants. Peripheral blood mononuclear cells were isolated and incubated in a solution containing phorbol-myristate-acetate (PMA) and ionomycin in the presence or absence of morphine or various ketamine concentrations (25, 50, and 100 µM). Samples were analyzed 4 h later. Th1 and Th2 cells were significantly increased by PMA and ionomycin stimulation; however, Th1 cells and the Th1/Th2 ratio in PMA and ionomycin treatments were significantly decreased in the CRC group compared with the control group. Following incubation with PMA and ionomycin, morphine significantly decreased Th1 cells and the Th1/Th2 ratio in the CRC group. Ketamine did not significantly affect levels of Th1 or Th2 cells or the Th1/Th2 ratio at a concentration of 25 µM; however, a significant increase in the Th1/Th2 ratio was observed at a concentration of 50 µM and, at 100 µM, a significant decrease in Th1 and Th2 cells and an increase in the Th1/Th2 ratio were observed. The present study suggests that CRC may shift the balance of Th1/Th2 towards Th2 by inducing an immunological response, morphine is able to suppress the differentiation of Th cells and decreases the Th1/Th2 ratio, and ketamine may affect the differentiation of Th cells in a dose-dependent manner.</description><subject>Analgesics</subject><subject>Antigens</subject><subject>Apoptosis</subject><subject>Care and treatment</subject><subject>Cell differentiation</subject><subject>Chemotherapy</subject><subject>Clinical medicine</subject><subject>Colorectal cancer</subject><subject>Cytokines</subject><subject>Dosage and administration</subject><subject>Drug therapy</subject><subject>Immunology</subject><subject>Ketamine</subject><subject>Lymphocytes</subject><subject>Morphine</subject><subject>Mortality</subject><subject>Narcotics</subject><subject>Pain</subject><subject>Patient outcomes</subject><subject>Radiation therapy</subject><subject>Studies</subject><subject>Substance abuse treatment</subject><subject>T cells</subject><subject>Tumors</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptksFvFSEQxjdGY5vao1dD4sXLPmF5sOzFpGmsmtT00p4Jjx261F1Yga3Rv76z6Wu1pnAAht98MJOvqt4yuuGqaz5CmTYNZWrTUi5eVIes7ZqaUSZe7ve0U-ygOs75huIQkiklXlcHnEqBW35Y_fke0zz4AMSEnvyAYqb1UBKYMkEoJC_znCBnUgYgvXcOEoa9KT4GEh25JAOMMyRiYRzzGpnxDpFMfvkyEBvHmMAWMxJrgkXQB3LrS4pvqlfOjBmO9-tRdXX2-fL0a31-8eXb6cl5bUWrSu3srrfM8h4oh61Qjgu6a43rQQG1tO0lUMqwAUZKRmELyvRC0KZTciuF5Pyo-nSvOy-7CXqLf0tm1HPyk0m_dTReP70JftDX8VZLTlsuOhT4sBdI8ecCuejJ57VcEyAuWTfYai5b7DWi7_9Db-KSApaHlGxaqhQTf6lrM4L2wUV8166i-kS0DLWUbJDaPEPh7GHyNgZwHuNPEur7BJtizgncY42M6tUvGv2iV7_o1S_Iv_u3MY_0gzv4HeDUvIY</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Hou, Min</creator><creator>Zhou, Naibao</creator><creator>Li, Hao</creator><creator>Wang, Baosheng</creator><creator>Wang, Xiuqin</creator><creator>Wang, Xingwu</creator><creator>Jiang, Tao</creator><creator>Wang, Kaiguo</creator><creator>Xue, Fushan</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190101</creationdate><title>Morphine and ketamine treatment suppress the differentiation of T helper cells of patients with colorectal cancer in vitro</title><author>Hou, Min ; Zhou, Naibao ; Li, Hao ; Wang, Baosheng ; Wang, Xiuqin ; Wang, Xingwu ; Jiang, Tao ; Wang, Kaiguo ; Xue, Fushan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c578t-fcbdc1c3de03e458f350b7afde8e0c07d6e001703a6610e4e8ad5502986465633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analgesics</topic><topic>Antigens</topic><topic>Apoptosis</topic><topic>Care and treatment</topic><topic>Cell differentiation</topic><topic>Chemotherapy</topic><topic>Clinical medicine</topic><topic>Colorectal cancer</topic><topic>Cytokines</topic><topic>Dosage and administration</topic><topic>Drug therapy</topic><topic>Immunology</topic><topic>Ketamine</topic><topic>Lymphocytes</topic><topic>Morphine</topic><topic>Mortality</topic><topic>Narcotics</topic><topic>Pain</topic><topic>Patient outcomes</topic><topic>Radiation therapy</topic><topic>Studies</topic><topic>Substance abuse treatment</topic><topic>T cells</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Hou, Min</creatorcontrib><creatorcontrib>Zhou, Naibao</creatorcontrib><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Wang, Baosheng</creatorcontrib><creatorcontrib>Wang, Xiuqin</creatorcontrib><creatorcontrib>Wang, Xingwu</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Wang, Kaiguo</creatorcontrib><creatorcontrib>Xue, Fushan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Min</au><au>Zhou, Naibao</au><au>Li, Hao</au><au>Wang, Baosheng</au><au>Wang, Xiuqin</au><au>Wang, Xingwu</au><au>Jiang, Tao</au><au>Wang, Kaiguo</au><au>Xue, Fushan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphine and ketamine treatment suppress the differentiation of T helper cells of patients with colorectal cancer in vitro</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>17</volume><issue>1</issue><spage>935</spage><epage>942</epage><pages>935-942</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>There have been conflicting reports regarding the effects of anesthetic and analgesic drugs on immune function in patients with cancer. The aim of the present study was to investigate changes to T helper (Th) cell populations in patients with colorectal cancer (CRC) and to assess the effects of morphine and ketamine on the differentiation of Th cells harvested from patients with CRC
. Peripheral blood samples were extracted from 20 patients with CRC and 20 healthy participants. Peripheral blood mononuclear cells were isolated and incubated in a solution containing phorbol-myristate-acetate (PMA) and ionomycin in the presence or absence of morphine or various ketamine concentrations (25, 50, and 100 µM). Samples were analyzed 4 h later. Th1 and Th2 cells were significantly increased by PMA and ionomycin stimulation; however, Th1 cells and the Th1/Th2 ratio in PMA and ionomycin treatments were significantly decreased in the CRC group compared with the control group. Following incubation with PMA and ionomycin, morphine significantly decreased Th1 cells and the Th1/Th2 ratio in the CRC group. Ketamine did not significantly affect levels of Th1 or Th2 cells or the Th1/Th2 ratio at a concentration of 25 µM; however, a significant increase in the Th1/Th2 ratio was observed at a concentration of 50 µM and, at 100 µM, a significant decrease in Th1 and Th2 cells and an increase in the Th1/Th2 ratio were observed. The present study suggests that CRC may shift the balance of Th1/Th2 towards Th2 by inducing an immunological response, morphine is able to suppress the differentiation of Th cells and decreases the Th1/Th2 ratio, and ketamine may affect the differentiation of Th cells in a dose-dependent manner.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>30651883</pmid><doi>10.3892/etm.2018.7035</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Analgesics Antigens Apoptosis Care and treatment Cell differentiation Chemotherapy Clinical medicine Colorectal cancer Cytokines Dosage and administration Drug therapy Immunology Ketamine Lymphocytes Morphine Mortality Narcotics Pain Patient outcomes Radiation therapy Studies Substance abuse treatment T cells Tumors |
title | Morphine and ketamine treatment suppress the differentiation of T helper cells of patients with colorectal cancer in vitro |
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