Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride
Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects...
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| description | Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects regeneration owing to deterioration of telomeric maintenance. We introduce
Aeolosoma viride—
a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of
A. viride
declines with age. We characterized the
A. viride
telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene
Avi-tert
. In adult
A. viride
, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of
Avi-tert
expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects
A. viride
regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in
A. viride
. |
| doi_str_mv | 10.1038/s41598-018-36396-y |
| format | Article |
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Aeolosoma viride—
a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of
A. viride
declines with age. We characterized the
A. viride
telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene
Avi-tert
. In adult
A. viride
, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of
Avi-tert
expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects
A. viride
regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in
A. viride
.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-36396-y</identifier><identifier>PMID: 30584242</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/32 ; 38 ; 38/22 ; 38/71 ; 45 ; 45/90 ; 631/181/735 ; 631/532/489 ; 631/80/103/560 ; Aeolosoma ; Aging ; Aging - genetics ; Animals ; Annelida - genetics ; Annelida - growth & development ; Annelida - physiology ; Cell proliferation ; Humanities and Social Sciences ; multidisciplinary ; Regeneration ; Science ; Science (multidisciplinary) ; Telomerase ; Telomerase - genetics ; Telomerase - metabolism ; Telomere Homeostasis ; Yeast</subject><ispartof>Scientific reports, 2018-12, Vol.8 (1), p.18078-13, Article 18078</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-7387202296aad2fb2111afe85569e4d49f04aaaaabc90bba0b86c95c9eb173ba3</citedby><cites>FETCH-LOGICAL-c511t-7387202296aad2fb2111afe85569e4d49f04aaaaabc90bba0b86c95c9eb173ba3</cites><orcidid>0000-0002-9857-068X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305377/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305377/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30584242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Chi-Fan</creatorcontrib><creatorcontrib>Sung, Tzu-Ling</creatorcontrib><creatorcontrib>Chen, Liuh-Yow</creatorcontrib><creatorcontrib>Chen, Jiun-Hong</creatorcontrib><title>Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects regeneration owing to deterioration of telomeric maintenance. We introduce
Aeolosoma viride—
a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of
A. viride
declines with age. We characterized the
A. viride
telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene
Avi-tert
. In adult
A. viride
, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of
Avi-tert
expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects
A. viride
regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in
A. viride
.</description><subject>14/32</subject><subject>38</subject><subject>38/22</subject><subject>38/71</subject><subject>45</subject><subject>45/90</subject><subject>631/181/735</subject><subject>631/532/489</subject><subject>631/80/103/560</subject><subject>Aeolosoma</subject><subject>Aging</subject><subject>Aging - genetics</subject><subject>Animals</subject><subject>Annelida - genetics</subject><subject>Annelida - growth & development</subject><subject>Annelida - physiology</subject><subject>Cell proliferation</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Regeneration</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Telomerase</subject><subject>Telomerase - genetics</subject><subject>Telomerase - metabolism</subject><subject>Telomere Homeostasis</subject><subject>Yeast</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1P3DAQhq2qqKBl_0APlaVeegn4K4l9qYRQC0hIXOBsTZLJrlFib-2Eav89XhYo9MBcbM08847HLyFfOTvhTOrTpHhpdMG4LmQlTVVsP5EjwVRZCCnE5zf3Q7JM6Z7lKIVR3Hwhh5KVWgkljkh_i0MYMSIdwfkJPfgWaTdH51cUciK6EGnEFXqMMLngc7ajsNrVnafTGmkfMa3_QmZzzePgOnqGYQgpjEAfXHQdHpODHoaEy-dzQe5-_7o9vyyuby6uzs-ui7bkfCpqqWvBhDAVQCf6RnDOoUddlpVB1SnTMwW7aFrDmgZYo6vWlK3BhteyAbkgP_e6m7kZsWvRTxEGu4luhLi1AZx9X_FubVfhwVb5S2RdZ4EfzwIx_JkxTXZ0qcVhAI9hTlbwislKC2Uy-v0_9D7M0ef1niiueK11psSeamNIKWL_-hjO7M5Ju3fSZiftk5N2m5u-vV3jteXFtwzIPZA2O6cw_pv9gewj0tCshg</recordid><startdate>20181224</startdate><enddate>20181224</enddate><creator>Chen, Chi-Fan</creator><creator>Sung, Tzu-Ling</creator><creator>Chen, Liuh-Yow</creator><creator>Chen, Jiun-Hong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9857-068X</orcidid></search><sort><creationdate>20181224</creationdate><title>Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride</title><author>Chen, Chi-Fan ; Sung, Tzu-Ling ; Chen, Liuh-Yow ; Chen, Jiun-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-7387202296aad2fb2111afe85569e4d49f04aaaaabc90bba0b86c95c9eb173ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>14/32</topic><topic>38</topic><topic>38/22</topic><topic>38/71</topic><topic>45</topic><topic>45/90</topic><topic>631/181/735</topic><topic>631/532/489</topic><topic>631/80/103/560</topic><topic>Aeolosoma</topic><topic>Aging</topic><topic>Aging - genetics</topic><topic>Animals</topic><topic>Annelida - genetics</topic><topic>Annelida - growth & development</topic><topic>Annelida - physiology</topic><topic>Cell proliferation</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Regeneration</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Telomerase</topic><topic>Telomerase - genetics</topic><topic>Telomerase - metabolism</topic><topic>Telomere Homeostasis</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Chi-Fan</creatorcontrib><creatorcontrib>Sung, Tzu-Ling</creatorcontrib><creatorcontrib>Chen, Liuh-Yow</creatorcontrib><creatorcontrib>Chen, Jiun-Hong</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Chi-Fan</au><au>Sung, Tzu-Ling</au><au>Chen, Liuh-Yow</au><au>Chen, Jiun-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-12-24</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>18078</spage><epage>13</epage><pages>18078-13</pages><artnum>18078</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Aging is a complex process involving declines in various cellular and physical functionalities, including regenerative ability. Telomere maintenance is thought to be necessary for regeneration, and telomere attrition is one mechanism that contributes to aging. However, it is unclear if aging affects regeneration owing to deterioration of telomeric maintenance. We introduce
Aeolosoma viride—
a freshwater annelid with strong regenerative abilities—as a new model for studying the effects of aging on telomere functions and regeneration. We show that the anterior regenerative ability of
A. viride
declines with age. We characterized the
A. viride
telomere sequence as being composed of TTAGGG repeats and identifyied the telomerase gene
Avi-tert
. In adult
A. viride
, telomerase was constantly active and telomere lengths were similar among different body sections and stably maintained with age. Notably, we found that regeneration did not result in telomere shortening at regenerating sites. Moreover, transient up-regulation of
Avi-tert
expression and telomerase activity was observed at regenerating sites, which might promote telomere lengthening to counteract telomere erosion resulting from cell proliferation. Our study suggests that although aging affects
A. viride
regeneration independent of steady-state telomere length, timely regulation of telomerase functions is critical for the regeneration process in
A. viride
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30584242</pmid><doi>10.1038/s41598-018-36396-y</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9857-068X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 14/32 38 38/22 38/71 45 45/90 631/181/735 631/532/489 631/80/103/560 Aeolosoma Aging Aging - genetics Animals Annelida - genetics Annelida - growth & development Annelida - physiology Cell proliferation Humanities and Social Sciences multidisciplinary Regeneration Science Science (multidisciplinary) Telomerase Telomerase - genetics Telomerase - metabolism Telomere Homeostasis Yeast |
| title | Telomere maintenance during anterior regeneration and aging in the freshwater annelid Aeolosoma viride |
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