Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii)
The hybrid sturgeon ( Huso dauricus × Acipenser schrenckii ) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study, de novo sequencing was used to compare the difference in transc...
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| Veröffentlicht in: | Scientific reports 2018-12, Vol.8 (1), p.17925-18, Article 17925 |
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| description | The hybrid sturgeon (
Huso dauricus
×
Acipenser schrenckii
) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study,
de novo
sequencing was used to compare the difference in transcriptome in spleen of the infected and mock infected sturgeon with
Aeromonas hydrophila
. Among 187,244 unigenes obtained, 87,887 unigenes were annotated and 1,147 unigenes were associated with immune responses genes. Comparative expression analysis indicated that 2,723 differently expressed genes between the infected and mock-infected group were identified, including 1,420 up-regulated and 1,303 down-regulated genes. 283 differently expressed anti-bacterial immune related genes were scrutinized, including 168 up-regulated and 115 down-regulated genes. Ten of the differently expressed genes were further validated by qRT-PCR. In this study, toll like receptors (TLRs) pathway, NF-kappa B pathway, class A scavenger receptor pathway, phagocytosis pathway, mannose receptor pathway and complement pathway were shown to be up-regulated in
Aeromonas hydrophila
infected hybrid sturgeon. Additionally, 65,040 potential SSRs and 2,133,505 candidate SNPs were identified from the hybrid sturgeon spleen transcriptome. This study could provide an insight of host immune genes associated with bacterial infection in hybrid sturgeon. |
| doi_str_mv | 10.1038/s41598-018-36376-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6298973</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2158239969</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-6dde51d2678c67aa0624646c22f0815c9b4d51d519dd72f23dbc04c195b436e23</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EolXpC7BAltiURcD_iTdIowooUiU2ZW05tjPjktjBN6k0T8ID8WK4TFsKC7y5ls_n43t9EHpJyVtKePcOBJW6awjtGq54qxr2BB0zImTDOGNPH-2P0CnANalLMi2ofo6OOJGKdB0_RtNVsQlcifOSp4BtsuMeIuA84E0oecrJAt7tfcnzLo4WxzQEtwRfz_oSPYZlLduQEz67WCFjb9cS3Qo_f2xcnEOCUDC4XQnJfYvxzQv0bLAjhNO7eoK-fvxwdX7RXH759Pl8c9k4SenSKO-DpJ6ptnOqtZYoJpRQjrGBdFQ63QtfdUm19y0bGPe9I8JRLXvBVWD8BL0_-M5rPwXvQlqKHc1c4mTL3mQbzd9KijuzzTdGMd3plleDszuDkr-vARYzRXBhHG0KeQXDqOwY11rpir7-B73Oa6n_eE-Jtm0rxQ6UKxmghOGhGUrMbaDmEKipgZrfgZrbMV49HuPhyn18FeAHAKqUtqH8efs_tr8ASmWucg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2158234777</pqid></control><display><type>article</type><title>Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii)</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Jiang, Nan ; Fan, Yuding ; Zhou, Yong ; Wang, Weiling ; Ma, Jie ; Zeng, Lingbing</creator><creatorcontrib>Jiang, Nan ; Fan, Yuding ; Zhou, Yong ; Wang, Weiling ; Ma, Jie ; Zeng, Lingbing</creatorcontrib><description>The hybrid sturgeon (
Huso dauricus
×
Acipenser schrenckii
) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study,
de novo
sequencing was used to compare the difference in transcriptome in spleen of the infected and mock infected sturgeon with
Aeromonas hydrophila
. Among 187,244 unigenes obtained, 87,887 unigenes were annotated and 1,147 unigenes were associated with immune responses genes. Comparative expression analysis indicated that 2,723 differently expressed genes between the infected and mock-infected group were identified, including 1,420 up-regulated and 1,303 down-regulated genes. 283 differently expressed anti-bacterial immune related genes were scrutinized, including 168 up-regulated and 115 down-regulated genes. Ten of the differently expressed genes were further validated by qRT-PCR. In this study, toll like receptors (TLRs) pathway, NF-kappa B pathway, class A scavenger receptor pathway, phagocytosis pathway, mannose receptor pathway and complement pathway were shown to be up-regulated in
Aeromonas hydrophila
infected hybrid sturgeon. Additionally, 65,040 potential SSRs and 2,133,505 candidate SNPs were identified from the hybrid sturgeon spleen transcriptome. This study could provide an insight of host immune genes associated with bacterial infection in hybrid sturgeon.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-36376-2</identifier><identifier>PMID: 30560883</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/250/2499 ; 631/326/41/2533 ; Acipenser schrenckii ; Aeromonas hydrophila ; Aeromonas hydrophila - immunology ; Aeromonas hydrophila - pathogenicity ; Animals ; Aquaculture ; Bacteria ; Bacterial diseases ; Economic importance ; Female ; Fish ; Fish Proteins - genetics ; Fishes - genetics ; Fishes - immunology ; Fishes - microbiology ; Gene expression ; Gene Expression Profiling - veterinary ; Gene Expression Regulation ; Gene Regulatory Networks ; Humanities and Social Sciences ; Huso dauricus ; Immune response ; Male ; Mannose ; Molecular Sequence Annotation ; multidisciplinary ; NF-κB protein ; Phagocytosis ; Polymorphism, Single Nucleotide ; Scavenger receptors ; Science ; Science (multidisciplinary) ; Sequence Analysis, RNA - veterinary ; Single-nucleotide polymorphism ; Spleen ; Spleen - chemistry ; Spleen - immunology ; Spleen - microbiology</subject><ispartof>Scientific reports, 2018-12, Vol.8 (1), p.17925-18, Article 17925</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-6dde51d2678c67aa0624646c22f0815c9b4d51d519dd72f23dbc04c195b436e23</citedby><cites>FETCH-LOGICAL-c511t-6dde51d2678c67aa0624646c22f0815c9b4d51d519dd72f23dbc04c195b436e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298973/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6298973/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30560883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Nan</creatorcontrib><creatorcontrib>Fan, Yuding</creatorcontrib><creatorcontrib>Zhou, Yong</creatorcontrib><creatorcontrib>Wang, Weiling</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Zeng, Lingbing</creatorcontrib><title>Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii)</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The hybrid sturgeon (
Huso dauricus
×
Acipenser schrenckii
) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study,
de novo
sequencing was used to compare the difference in transcriptome in spleen of the infected and mock infected sturgeon with
Aeromonas hydrophila
. Among 187,244 unigenes obtained, 87,887 unigenes were annotated and 1,147 unigenes were associated with immune responses genes. Comparative expression analysis indicated that 2,723 differently expressed genes between the infected and mock-infected group were identified, including 1,420 up-regulated and 1,303 down-regulated genes. 283 differently expressed anti-bacterial immune related genes were scrutinized, including 168 up-regulated and 115 down-regulated genes. Ten of the differently expressed genes were further validated by qRT-PCR. In this study, toll like receptors (TLRs) pathway, NF-kappa B pathway, class A scavenger receptor pathway, phagocytosis pathway, mannose receptor pathway and complement pathway were shown to be up-regulated in
Aeromonas hydrophila
infected hybrid sturgeon. Additionally, 65,040 potential SSRs and 2,133,505 candidate SNPs were identified from the hybrid sturgeon spleen transcriptome. This study could provide an insight of host immune genes associated with bacterial infection in hybrid sturgeon.</description><subject>631/250/2499</subject><subject>631/326/41/2533</subject><subject>Acipenser schrenckii</subject><subject>Aeromonas hydrophila</subject><subject>Aeromonas hydrophila - immunology</subject><subject>Aeromonas hydrophila - pathogenicity</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Economic importance</subject><subject>Female</subject><subject>Fish</subject><subject>Fish Proteins - genetics</subject><subject>Fishes - genetics</subject><subject>Fishes - immunology</subject><subject>Fishes - microbiology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - veterinary</subject><subject>Gene Expression Regulation</subject><subject>Gene Regulatory Networks</subject><subject>Humanities and Social Sciences</subject><subject>Huso dauricus</subject><subject>Immune response</subject><subject>Male</subject><subject>Mannose</subject><subject>Molecular Sequence Annotation</subject><subject>multidisciplinary</subject><subject>NF-κB protein</subject><subject>Phagocytosis</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Scavenger receptors</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sequence Analysis, RNA - veterinary</subject><subject>Single-nucleotide polymorphism</subject><subject>Spleen</subject><subject>Spleen - chemistry</subject><subject>Spleen - immunology</subject><subject>Spleen - microbiology</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1DAUhS0EolXpC7BAltiURcD_iTdIowooUiU2ZW05tjPjktjBN6k0T8ID8WK4TFsKC7y5ls_n43t9EHpJyVtKePcOBJW6awjtGq54qxr2BB0zImTDOGNPH-2P0CnANalLMi2ofo6OOJGKdB0_RtNVsQlcifOSp4BtsuMeIuA84E0oecrJAt7tfcnzLo4WxzQEtwRfz_oSPYZlLduQEz67WCFjb9cS3Qo_f2xcnEOCUDC4XQnJfYvxzQv0bLAjhNO7eoK-fvxwdX7RXH759Pl8c9k4SenSKO-DpJ6ptnOqtZYoJpRQjrGBdFQ63QtfdUm19y0bGPe9I8JRLXvBVWD8BL0_-M5rPwXvQlqKHc1c4mTL3mQbzd9KijuzzTdGMd3plleDszuDkr-vARYzRXBhHG0KeQXDqOwY11rpir7-B73Oa6n_eE-Jtm0rxQ6UKxmghOGhGUrMbaDmEKipgZrfgZrbMV49HuPhyn18FeAHAKqUtqH8efs_tr8ASmWucg</recordid><startdate>20181218</startdate><enddate>20181218</enddate><creator>Jiang, Nan</creator><creator>Fan, Yuding</creator><creator>Zhou, Yong</creator><creator>Wang, Weiling</creator><creator>Ma, Jie</creator><creator>Zeng, Lingbing</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20181218</creationdate><title>Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii)</title><author>Jiang, Nan ; Fan, Yuding ; Zhou, Yong ; Wang, Weiling ; Ma, Jie ; Zeng, Lingbing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-6dde51d2678c67aa0624646c22f0815c9b4d51d519dd72f23dbc04c195b436e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>631/250/2499</topic><topic>631/326/41/2533</topic><topic>Acipenser schrenckii</topic><topic>Aeromonas hydrophila</topic><topic>Aeromonas hydrophila - immunology</topic><topic>Aeromonas hydrophila - pathogenicity</topic><topic>Animals</topic><topic>Aquaculture</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Economic importance</topic><topic>Female</topic><topic>Fish</topic><topic>Fish Proteins - genetics</topic><topic>Fishes - genetics</topic><topic>Fishes - immunology</topic><topic>Fishes - microbiology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - veterinary</topic><topic>Gene Expression Regulation</topic><topic>Gene Regulatory Networks</topic><topic>Humanities and Social Sciences</topic><topic>Huso dauricus</topic><topic>Immune response</topic><topic>Male</topic><topic>Mannose</topic><topic>Molecular Sequence Annotation</topic><topic>multidisciplinary</topic><topic>NF-κB protein</topic><topic>Phagocytosis</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Scavenger receptors</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sequence Analysis, RNA - veterinary</topic><topic>Single-nucleotide polymorphism</topic><topic>Spleen</topic><topic>Spleen - chemistry</topic><topic>Spleen - immunology</topic><topic>Spleen - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Nan</creatorcontrib><creatorcontrib>Fan, Yuding</creatorcontrib><creatorcontrib>Zhou, Yong</creatorcontrib><creatorcontrib>Wang, Weiling</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Zeng, Lingbing</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Nan</au><au>Fan, Yuding</au><au>Zhou, Yong</au><au>Wang, Weiling</au><au>Ma, Jie</au><au>Zeng, Lingbing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii)</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-12-18</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>17925</spage><epage>18</epage><pages>17925-18</pages><artnum>17925</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The hybrid sturgeon (
Huso dauricus
×
Acipenser schrenckii
) is an economically important species in China. With the increasing aquaculture of hybrid sturgeon, the bacterial diseases are a great concern of the industry. In this study,
de novo
sequencing was used to compare the difference in transcriptome in spleen of the infected and mock infected sturgeon with
Aeromonas hydrophila
. Among 187,244 unigenes obtained, 87,887 unigenes were annotated and 1,147 unigenes were associated with immune responses genes. Comparative expression analysis indicated that 2,723 differently expressed genes between the infected and mock-infected group were identified, including 1,420 up-regulated and 1,303 down-regulated genes. 283 differently expressed anti-bacterial immune related genes were scrutinized, including 168 up-regulated and 115 down-regulated genes. Ten of the differently expressed genes were further validated by qRT-PCR. In this study, toll like receptors (TLRs) pathway, NF-kappa B pathway, class A scavenger receptor pathway, phagocytosis pathway, mannose receptor pathway and complement pathway were shown to be up-regulated in
Aeromonas hydrophila
infected hybrid sturgeon. Additionally, 65,040 potential SSRs and 2,133,505 candidate SNPs were identified from the hybrid sturgeon spleen transcriptome. This study could provide an insight of host immune genes associated with bacterial infection in hybrid sturgeon.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30560883</pmid><doi>10.1038/s41598-018-36376-2</doi><tpages>18</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/250/2499 631/326/41/2533 Acipenser schrenckii Aeromonas hydrophila Aeromonas hydrophila - immunology Aeromonas hydrophila - pathogenicity Animals Aquaculture Bacteria Bacterial diseases Economic importance Female Fish Fish Proteins - genetics Fishes - genetics Fishes - immunology Fishes - microbiology Gene expression Gene Expression Profiling - veterinary Gene Expression Regulation Gene Regulatory Networks Humanities and Social Sciences Huso dauricus Immune response Male Mannose Molecular Sequence Annotation multidisciplinary NF-κB protein Phagocytosis Polymorphism, Single Nucleotide Scavenger receptors Science Science (multidisciplinary) Sequence Analysis, RNA - veterinary Single-nucleotide polymorphism Spleen Spleen - chemistry Spleen - immunology Spleen - microbiology |
| title | Transcriptome analysis of Aeromonas hydrophila infected hybrid sturgeon (Huso dauricus×Acipenser schrenckii) |
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