In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep
[Display omitted] Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape poma...
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| Veröffentlicht in: | International journal of veterinary science and medicine 2018-12, Vol.6 (2), p.243-247 |
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| creator | Silva Soares, Suelen Carolina de Lima, Gilmar Cotrin Carlos Laurentiz, Antonio Féboli, Aline dos Anjos, Luciano Alves de Paula Carlis, Matheus Souza da Silva Filardi, Rosimeire da Silva de Laurentiz, Rosangela |
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Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep. |
| doi_str_mv | 10.1016/j.ijvsm.2018.11.005 |
| format | Article |
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Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep.</description><identifier>ISSN: 2314-4599</identifier><identifier>ISSN: 2314-4580</identifier><identifier>EISSN: 2314-4599</identifier><identifier>DOI: 10.1016/j.ijvsm.2018.11.005</identifier><identifier>PMID: 30564603</identifier><language>eng</language><publisher>United States: Elsevier B.V</publisher><subject>anthelmintics ; byproducts ; eggs ; flavonoids ; Gastrointestinal nematodes ; grape pomace ; grape pomace extracts ; grapes ; Haemonchus contortus ; hatching ; in vitro studies ; larvae ; larval development ; larvicides ; lethal dose 50 ; Nematoda ; nematode control ; nutrient content ; nutrients ; Ovicidal and larvicidal activities ; Phytochemical ; processing technology ; saponins ; sheep ; Short Communication ; Tannin ; tannins</subject><ispartof>International journal of veterinary science and medicine, 2018-12, Vol.6 (2), p.243-247</ispartof><rights>2018 Faculty of Veterinary Medicine, Cairo University</rights><rights>Faculty of Veterinary Medicine, Cairo University 2018</rights><rights>2018 Faculty of Veterinary Medicine, Cairo University. Published by Elsevier B.V. 2018 Faculty of Veterinary Medicine, Cairo University</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c613t-1b75b4edf6c53c814c45930d216707025f86bc2db8eabcae701926ef04a6c7ce3</citedby><cites>FETCH-LOGICAL-c613t-1b75b4edf6c53c814c45930d216707025f86bc2db8eabcae701926ef04a6c7ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286422/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6286422/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27479,27901,27902,53766,53768,59116,59117</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30564603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva Soares, Suelen Carolina</creatorcontrib><creatorcontrib>de Lima, Gilmar Cotrin</creatorcontrib><creatorcontrib>Carlos Laurentiz, Antonio</creatorcontrib><creatorcontrib>Féboli, Aline</creatorcontrib><creatorcontrib>dos Anjos, Luciano Alves</creatorcontrib><creatorcontrib>de Paula Carlis, Matheus Souza</creatorcontrib><creatorcontrib>da Silva Filardi, Rosimeire</creatorcontrib><creatorcontrib>da Silva de Laurentiz, Rosangela</creatorcontrib><title>In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep</title><title>International journal of veterinary science and medicine</title><addtitle>Int J Vet Sci Med</addtitle><description>[Display omitted]
Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep.</description><subject>anthelmintics</subject><subject>byproducts</subject><subject>eggs</subject><subject>flavonoids</subject><subject>Gastrointestinal nematodes</subject><subject>grape pomace</subject><subject>grape pomace extracts</subject><subject>grapes</subject><subject>Haemonchus contortus</subject><subject>hatching</subject><subject>in vitro studies</subject><subject>larvae</subject><subject>larval development</subject><subject>larvicides</subject><subject>lethal dose 50</subject><subject>Nematoda</subject><subject>nematode control</subject><subject>nutrient content</subject><subject>nutrients</subject><subject>Ovicidal and larvicidal activities</subject><subject>Phytochemical</subject><subject>processing technology</subject><subject>saponins</subject><subject>sheep</subject><subject>Short Communication</subject><subject>Tannin</subject><subject>tannins</subject><issn>2314-4599</issn><issn>2314-4580</issn><issn>2314-4599</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNqNUk1vEzEQXSEQrUJ_ARLykUuCP3bt3QNIqOIjUiUucLZmvbOJI6-92E4g_x6nKaW9VPhia2be87yZV1WvGV0xyuS73cruDmlaccraFWMrSptn1SUXrF7WTdc9f_C-qK5S2tFyOkUFZS-rC0EbWUsqLqv92pODzTEQ8HmLbrI-W0PAZFvCRxJGsokwI5nDBAYJ_s6xJAlswPqUyQZSARcQpmw9OOJxghwGTCeoh7yP4NyRWD-iyTiQtEWcX1UvRnAJr-7uRfXj86fv11-XN9--rK8_3iyNZCIvWa-avsZhlKYRpmW1KXoEHTiTiirKm7GVveFD3yL0BlBR1nGJI61BGmVQLKr1mXcIsNNztBPEow5g9W0gxI2GWPQ61FyxoecwCma6WinaM2j7jolaNW3fd2Ph-nDmmvf9hINBXybhHpE-zni71Ztw0JK3sua8ELy9I4jh577MS082GXQOPIZ90pxz2naqlf9Rypq2aTpVprGoxLnUxJBSxPG-I0b1ySp6p2-tok9W0YzpYpWCevNQzD3mrzH-qS2LC3GCXyG6QWc4uhDHCN7YpMXTP7w_E2DZ78Fi1MlY9AYHG4sTygLskx3-AdtW6Ro</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Silva Soares, Suelen Carolina</creator><creator>de Lima, Gilmar Cotrin</creator><creator>Carlos Laurentiz, Antonio</creator><creator>Féboli, Aline</creator><creator>dos Anjos, Luciano Alves</creator><creator>de Paula Carlis, Matheus Souza</creator><creator>da Silva Filardi, Rosimeire</creator><creator>da Silva de Laurentiz, Rosangela</creator><general>Elsevier B.V</general><general>Taylor & Francis</general><general>Faculty of Veterinary Medicine, Cairo University</general><general>Taylor & Francis Group</general><scope>6I.</scope><scope>AAFTH</scope><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20181201</creationdate><title>In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep</title><author>Silva Soares, Suelen Carolina ; de Lima, Gilmar Cotrin ; Carlos Laurentiz, Antonio ; Féboli, Aline ; dos Anjos, Luciano Alves ; de Paula Carlis, Matheus Souza ; da Silva Filardi, Rosimeire ; da Silva de Laurentiz, Rosangela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c613t-1b75b4edf6c53c814c45930d216707025f86bc2db8eabcae701926ef04a6c7ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>anthelmintics</topic><topic>byproducts</topic><topic>eggs</topic><topic>flavonoids</topic><topic>Gastrointestinal nematodes</topic><topic>grape pomace</topic><topic>grape pomace extracts</topic><topic>grapes</topic><topic>Haemonchus contortus</topic><topic>hatching</topic><topic>in vitro studies</topic><topic>larvae</topic><topic>larval development</topic><topic>larvicides</topic><topic>lethal dose 50</topic><topic>Nematoda</topic><topic>nematode control</topic><topic>nutrient content</topic><topic>nutrients</topic><topic>Ovicidal and larvicidal activities</topic><topic>Phytochemical</topic><topic>processing technology</topic><topic>saponins</topic><topic>sheep</topic><topic>Short Communication</topic><topic>Tannin</topic><topic>tannins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva Soares, Suelen Carolina</creatorcontrib><creatorcontrib>de Lima, Gilmar Cotrin</creatorcontrib><creatorcontrib>Carlos Laurentiz, Antonio</creatorcontrib><creatorcontrib>Féboli, Aline</creatorcontrib><creatorcontrib>dos Anjos, Luciano Alves</creatorcontrib><creatorcontrib>de Paula Carlis, Matheus Souza</creatorcontrib><creatorcontrib>da Silva Filardi, Rosimeire</creatorcontrib><creatorcontrib>da Silva de Laurentiz, Rosangela</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Taylor & Francis Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International journal of veterinary science and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva Soares, Suelen Carolina</au><au>de Lima, Gilmar Cotrin</au><au>Carlos Laurentiz, Antonio</au><au>Féboli, Aline</au><au>dos Anjos, Luciano Alves</au><au>de Paula Carlis, Matheus Souza</au><au>da Silva Filardi, Rosimeire</au><au>da Silva de Laurentiz, Rosangela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep</atitle><jtitle>International journal of veterinary science and medicine</jtitle><addtitle>Int J Vet Sci Med</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>6</volume><issue>2</issue><spage>243</spage><epage>247</epage><pages>243-247</pages><issn>2314-4599</issn><issn>2314-4580</issn><eissn>2314-4599</eissn><abstract>[Display omitted]
Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep.</abstract><cop>United States</cop><pub>Elsevier B.V</pub><pmid>30564603</pmid><doi>10.1016/j.ijvsm.2018.11.005</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | anthelmintics byproducts eggs flavonoids Gastrointestinal nematodes grape pomace grape pomace extracts grapes Haemonchus contortus hatching in vitro studies larvae larval development larvicides lethal dose 50 Nematoda nematode control nutrient content nutrients Ovicidal and larvicidal activities Phytochemical processing technology saponins sheep Short Communication Tannin tannins |
| title | In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep |
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