miRNA in plasma exosome is stable under different storage conditions
Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored sampl...
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| Veröffentlicht in: | Molecules (Basel, Switzerland) Switzerland), 2014-01, Vol.19 (2), p.1568-1575 |
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| creator | Ge, Qinyu Zhou, Youxia Lu, Jiafeng Bai, Yunfei Xie, Xueying Lu, Zuhong |
| description | Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored samples could be used for circulating RNA investigation because of RNase is unknown. The aim of the study was to determine the stability of circulating miRNA in exosomes and plasma. Exosomes were isolated from plasma samples by using ExoQuick Precipitation methods. RNA was extracted from exosomes and the corresponding plasma samples with a Qiagen miRNeasy Mini kit. The concentration of RNA was measured by a Qubit® RNA HS Assay Kit, and quantitative PCR was used for individual miRNA expression level detection. Results showed that exosomal miRNA showed extra stability under different storage conditions and no significant influence on plasma miRNA, except for short term storage at 4 °C. It is thus indicated that exosome miRNAs can be good biomarkers based on their stability under various storage conditions. |
| doi_str_mv | 10.3390/molecules19021568 |
| format | Article |
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Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored samples could be used for circulating RNA investigation because of RNase is unknown. The aim of the study was to determine the stability of circulating miRNA in exosomes and plasma. Exosomes were isolated from plasma samples by using ExoQuick Precipitation methods. RNA was extracted from exosomes and the corresponding plasma samples with a Qiagen miRNeasy Mini kit. The concentration of RNA was measured by a Qubit® RNA HS Assay Kit, and quantitative PCR was used for individual miRNA expression level detection. Results showed that exosomal miRNA showed extra stability under different storage conditions and no significant influence on plasma miRNA, except for short term storage at 4 °C. It is thus indicated that exosome miRNAs can be good biomarkers based on their stability under various storage conditions.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules19021568</identifier><identifier>PMID: 24473213</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biomarkers ; Biomarkers - blood ; Exosomes - metabolism ; Humans ; Investigations ; Laboratories ; MicroRNAs ; MicroRNAs - blood ; Nanoparticles ; Plasma ; RNA - chemistry ; RNA Stability ; RNA, Messenger - blood ; RNA, Messenger - chemistry ; Temperature</subject><ispartof>Molecules (Basel, Switzerland), 2014-01, Vol.19 (2), p.1568-1575</ispartof><rights>Copyright MDPI AG 2014</rights><rights>2014 by the authors. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-3e32974dcb19e0b928e8e5ac6aa6b540aa307dba5ff3a1ece79dea6e96f3a0af3</citedby><cites>FETCH-LOGICAL-c526t-3e32974dcb19e0b928e8e5ac6aa6b540aa307dba5ff3a1ece79dea6e96f3a0af3</cites><orcidid>0000-0002-1708-1949</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271968/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271968/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24473213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Qinyu</creatorcontrib><creatorcontrib>Zhou, Youxia</creatorcontrib><creatorcontrib>Lu, Jiafeng</creatorcontrib><creatorcontrib>Bai, Yunfei</creatorcontrib><creatorcontrib>Xie, Xueying</creatorcontrib><creatorcontrib>Lu, Zuhong</creatorcontrib><title>miRNA in plasma exosome is stable under different storage conditions</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored samples could be used for circulating RNA investigation because of RNase is unknown. The aim of the study was to determine the stability of circulating miRNA in exosomes and plasma. Exosomes were isolated from plasma samples by using ExoQuick Precipitation methods. RNA was extracted from exosomes and the corresponding plasma samples with a Qiagen miRNeasy Mini kit. The concentration of RNA was measured by a Qubit® RNA HS Assay Kit, and quantitative PCR was used for individual miRNA expression level detection. Results showed that exosomal miRNA showed extra stability under different storage conditions and no significant influence on plasma miRNA, except for short term storage at 4 °C. It is thus indicated that exosome miRNAs can be good biomarkers based on their stability under various storage conditions.</description><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Exosomes - metabolism</subject><subject>Humans</subject><subject>Investigations</subject><subject>Laboratories</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>Nanoparticles</subject><subject>Plasma</subject><subject>RNA - chemistry</subject><subject>RNA Stability</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - chemistry</subject><subject>Temperature</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1LAzEQhoMotlZ_gBdZ8OKlmmyyH7kIxW8oCqLnMJudrSm7m5rsiv57I62l6sHTfOSdh8m8hBwyesq5pGeNrVH3NXomacySNN8iQyZiOuZUyO2NfED2vJ_TIBIs2SWDWIiMx4wPyWVjHu8nkWmjRQ2-gQjfrbcNRsZHvoOixqhvS3RRaaoKHbZdaFsHM4y0bUvTGdv6fbJTQe3xYBVH5Pn66unidjx9uLm7mEzHOonTbsyRxzITpS6YRFrIOMccE9ApQFokggJwmpUFJFXFgaHGTJYIKco01BQqPiLnS-6iLxosddjGQa0WzjTgPpQFo36-tOZFzeybSuOMyTQPgJMVwNnXHn2nGuM11jW0aHuvWCIEZeE02f9SIQOUM_olPf4lndveteESAciZzLMkxBFhS5V21nuH1XpvRtWXneqPnWHmaPPD64lv__gnkEue3A</recordid><startdate>20140127</startdate><enddate>20140127</enddate><creator>Ge, Qinyu</creator><creator>Zhou, Youxia</creator><creator>Lu, Jiafeng</creator><creator>Bai, Yunfei</creator><creator>Xie, Xueying</creator><creator>Lu, Zuhong</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1708-1949</orcidid></search><sort><creationdate>20140127</creationdate><title>miRNA in plasma exosome is stable under different storage conditions</title><author>Ge, Qinyu ; Zhou, Youxia ; Lu, Jiafeng ; Bai, Yunfei ; Xie, Xueying ; Lu, Zuhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-3e32974dcb19e0b928e8e5ac6aa6b540aa307dba5ff3a1ece79dea6e96f3a0af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>Exosomes - metabolism</topic><topic>Humans</topic><topic>Investigations</topic><topic>Laboratories</topic><topic>MicroRNAs</topic><topic>MicroRNAs - blood</topic><topic>Nanoparticles</topic><topic>Plasma</topic><topic>RNA - chemistry</topic><topic>RNA Stability</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - chemistry</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Qinyu</creatorcontrib><creatorcontrib>Zhou, Youxia</creatorcontrib><creatorcontrib>Lu, Jiafeng</creatorcontrib><creatorcontrib>Bai, Yunfei</creatorcontrib><creatorcontrib>Xie, Xueying</creatorcontrib><creatorcontrib>Lu, Zuhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Qinyu</au><au>Zhou, Youxia</au><au>Lu, Jiafeng</au><au>Bai, Yunfei</au><au>Xie, Xueying</au><au>Lu, Zuhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miRNA in plasma exosome is stable under different storage conditions</atitle><jtitle>Molecules (Basel, Switzerland)</jtitle><addtitle>Molecules</addtitle><date>2014-01-27</date><risdate>2014</risdate><volume>19</volume><issue>2</issue><spage>1568</spage><epage>1575</epage><pages>1568-1575</pages><issn>1420-3049</issn><eissn>1420-3049</eissn><abstract>Exosomes are small membrane-bound vesicles secreted by most cell types. Exosomes contain various functional proteins, mRNAs and microRNAs (miRNAs) that could be used for diagnostic and therapeutic purposes. How we should store the samples before RNA isolation and whether those long term stored samples could be used for circulating RNA investigation because of RNase is unknown. The aim of the study was to determine the stability of circulating miRNA in exosomes and plasma. Exosomes were isolated from plasma samples by using ExoQuick Precipitation methods. RNA was extracted from exosomes and the corresponding plasma samples with a Qiagen miRNeasy Mini kit. The concentration of RNA was measured by a Qubit® RNA HS Assay Kit, and quantitative PCR was used for individual miRNA expression level detection. Results showed that exosomal miRNA showed extra stability under different storage conditions and no significant influence on plasma miRNA, except for short term storage at 4 °C. It is thus indicated that exosome miRNAs can be good biomarkers based on their stability under various storage conditions.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>24473213</pmid><doi>10.3390/molecules19021568</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1708-1949</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarkers Biomarkers - blood Exosomes - metabolism Humans Investigations Laboratories MicroRNAs MicroRNAs - blood Nanoparticles Plasma RNA - chemistry RNA Stability RNA, Messenger - blood RNA, Messenger - chemistry Temperature |
| title | miRNA in plasma exosome is stable under different storage conditions |
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