Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold
Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in t...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2018-12, Vol.70 (6), p.1487-1498 |
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| creator | Kazem-Arki, Mandana Kabiri, Mahboubeh Rad, Iman Roodbari, Nasim Hayati Hosseinpoor, Hoorieh Mirzaei, Samaneh Parivar, Kazem Hanaee-Ahvaz, Hana |
| description | Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors. |
| doi_str_mv | 10.1007/s10616-018-0226-4 |
| format | Article |
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Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-018-0226-4</identifier><identifier>PMID: 30083791</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Autografts ; Biochemistry ; Biomedicine ; Biotechnology ; Blood platelets ; Body fat ; Bone growth ; Bones ; Calcium phosphates ; Cell culture ; Cell differentiation ; Cell growth ; Chemistry ; Chemistry and Materials Science ; Coatings ; Culture media ; Growth factors ; Liposuction ; Mesenchymal stem cells ; Mesenchyme ; Original ; Original Article ; Osteogenesis ; Penicillin ; Plasma ; Polymers ; Polyvinyl alcohol ; Regeneration ; Stem cells ; Surgeons ; Tissue engineering</subject><ispartof>Cytotechnology (Dordrecht), 2018-12, Vol.70 (6), p.1487-1498</ispartof><rights>Springer Science+Business Media B.V., part of Springer Nature 2018</rights><rights>Copyright Springer Science & Business Media 2018</rights><rights>Springer Science+Business Media B.V., part of Springer Nature 2018.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-81bb476e38fb7bc29c10e53287d50c58d9f7a39c3ecac831773ef3532d97417c3</citedby><cites>FETCH-LOGICAL-c535t-81bb476e38fb7bc29c10e53287d50c58d9f7a39c3ecac831773ef3532d97417c3</cites><orcidid>0000-0001-9249-4279</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269372/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918272529?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,21367,27901,27902,33721,33722,41464,42533,43781,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30083791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kazem-Arki, Mandana</creatorcontrib><creatorcontrib>Kabiri, Mahboubeh</creatorcontrib><creatorcontrib>Rad, Iman</creatorcontrib><creatorcontrib>Roodbari, Nasim Hayati</creatorcontrib><creatorcontrib>Hosseinpoor, Hoorieh</creatorcontrib><creatorcontrib>Mirzaei, Samaneh</creatorcontrib><creatorcontrib>Parivar, Kazem</creatorcontrib><creatorcontrib>Hanaee-Ahvaz, Hana</creatorcontrib><title>Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.</description><subject>Autografts</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Blood platelets</subject><subject>Body fat</subject><subject>Bone growth</subject><subject>Bones</subject><subject>Calcium phosphates</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell growth</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Coatings</subject><subject>Culture media</subject><subject>Growth factors</subject><subject>Liposuction</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>Original</subject><subject>Original Article</subject><subject>Osteogenesis</subject><subject>Penicillin</subject><subject>Plasma</subject><subject>Polymers</subject><subject>Polyvinyl alcohol</subject><subject>Regeneration</subject><subject>Stem cells</subject><subject>Surgeons</subject><subject>Tissue engineering</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU1rFTEYhYMo9lr9AW5kwI2b6JtkZpJsBCn1AwoW0XXIJG9uU2aSazK30H9vLrfWD9BVIOc5JzkcQp4zeM0A5JvKYGQjBaYocD7S_gHZsEEKClKqh2QDmgPVMOoT8qTWawDQkonH5EQAKCE125D5PF3Z5HDBtHY5dLmumLeYout8DAFLu492jTkdVOvjLlekHku8Qd81eOkcznPtptvu8stlt-Rmi01KNuUQp5L3tavOhpBn_5Q8Cnau-OzuPCXf3p9_PftILz5_-HT27oK6QQwrVWyaejmiUGGSk-PaMcBBcCX9AG5QXgdphXYCnXVKMCkFBtEAr2XPpBOn5O0xd7efFvSudSh2NrsSF1tuTbbR_KmkeGW2-caMfNRC8hbw6i6g5O97rKtZYj30tAlbIcNB9ZqNUuuGvvwLvc77klo9wzVTXPKB_59iPbQoPkCj2JFyJddaMNx_mYE5LG6Oi5u2uDksbvrmefF713vHz4kbwI9AbVLaYvn19L9TfwAg1LdF</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Kazem-Arki, Mandana</creator><creator>Kabiri, Mahboubeh</creator><creator>Rad, Iman</creator><creator>Roodbari, Nasim Hayati</creator><creator>Hosseinpoor, Hoorieh</creator><creator>Mirzaei, Samaneh</creator><creator>Parivar, Kazem</creator><creator>Hanaee-Ahvaz, Hana</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9249-4279</orcidid></search><sort><creationdate>20181201</creationdate><title>Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold</title><author>Kazem-Arki, Mandana ; 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The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30083791</pmid><doi>10.1007/s10616-018-0226-4</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9249-4279</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Autografts Biochemistry Biomedicine Biotechnology Blood platelets Body fat Bone growth Bones Calcium phosphates Cell culture Cell differentiation Cell growth Chemistry Chemistry and Materials Science Coatings Culture media Growth factors Liposuction Mesenchymal stem cells Mesenchyme Original Original Article Osteogenesis Penicillin Plasma Polymers Polyvinyl alcohol Regeneration Stem cells Surgeons Tissue engineering |
| title | Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold |
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