Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis
Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multip...
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| Veröffentlicht in: | VirusDisease 2018-12, Vol.29 (4), p.540-543 |
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| creator | Marashi, Sayed Mahdi Mostafa, Aliehossadat Shoja, Zabihollah Nejati, Ahmad Shahmahmoodi, Shohreh Mollaei-Kandelous, Yaghoub Sahraian, Mohammad Ali Jalilvand, Somayeh |
| description | Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (
P
= 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group. |
| doi_str_mv | 10.1007/s13337-018-0481-1 |
| format | Article |
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P
= 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.</description><identifier>ISSN: 2347-3584</identifier><identifier>EISSN: 2347-3517</identifier><identifier>DOI: 10.1007/s13337-018-0481-1</identifier><identifier>PMID: 30539059</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Acquired immune deficiency syndrome ; AIDS ; Autoimmune diseases ; Biochemistry ; Biomedical and Life Sciences ; Brain research ; Care and treatment ; Cell Biology ; Disease ; DNA ; DNA sequencing ; Family medical history ; Genes ; Genomes ; Genomics ; Genotype & phenotype ; Genotypes ; Genotyping ; Health aspects ; Herpes viruses ; HIV ; Human immunodeficiency virus ; Infection ; K1 gene ; Life Sciences ; Lymphoma ; Maximum likelihood method ; Microbiology ; Multiple sclerosis ; Nucleotide sequence ; Nucleotide sequencing ; Peripheral blood ; Phylogenetics ; Polymerase chain reaction ; Protein Structure ; Sarcoma ; Short Communication ; Statistical analysis ; Viral infections</subject><ispartof>VirusDisease, 2018-12, Vol.29 (4), p.540-543</ispartof><rights>Indian Virological Society 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Indian Virological Society 2018.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4521-a283d992c9104ff400b4479248317ab27fcbe1c2f5f58b95e641a09444283e213</citedby><cites>FETCH-LOGICAL-c4521-a283d992c9104ff400b4479248317ab27fcbe1c2f5f58b95e641a09444283e213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261899/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2919606493?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,21389,27924,27925,33530,33531,33744,33745,41488,42557,43659,43805,51319,53791,53793,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30539059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marashi, Sayed Mahdi</creatorcontrib><creatorcontrib>Mostafa, Aliehossadat</creatorcontrib><creatorcontrib>Shoja, Zabihollah</creatorcontrib><creatorcontrib>Nejati, Ahmad</creatorcontrib><creatorcontrib>Shahmahmoodi, Shohreh</creatorcontrib><creatorcontrib>Mollaei-Kandelous, Yaghoub</creatorcontrib><creatorcontrib>Sahraian, Mohammad Ali</creatorcontrib><creatorcontrib>Jalilvand, Somayeh</creatorcontrib><title>Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis</title><title>VirusDisease</title><addtitle>VirusDis</addtitle><addtitle>Virusdisease</addtitle><description>Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (
P
= 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. 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A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (
P
= 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>30539059</pmid><doi>10.1007/s13337-018-0481-1</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acquired immune deficiency syndrome AIDS Autoimmune diseases Biochemistry Biomedical and Life Sciences Brain research Care and treatment Cell Biology Disease DNA DNA sequencing Family medical history Genes Genomes Genomics Genotype & phenotype Genotypes Genotyping Health aspects Herpes viruses HIV Human immunodeficiency virus Infection K1 gene Life Sciences Lymphoma Maximum likelihood method Microbiology Multiple sclerosis Nucleotide sequence Nucleotide sequencing Peripheral blood Phylogenetics Polymerase chain reaction Protein Structure Sarcoma Short Communication Statistical analysis Viral infections |
| title | Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis |
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