Magnetic beads-based neuraminidase enzyme microreactor as a drug discovery tool for screening inhibitors from compound libraries and fishing ligands from natural products

•Neuraminidase functionalized-magnetic beads (NA-MBs) were prepared for the first time.•A screening assay for pure compound libraries was established using NA-MBs coupled with LC–MS/MS.•NA-MBs were successfully applied for the NA ligand fishing from Flos Lonicerae.•The NA-MBs based assay offers a no...

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Veröffentlicht in:	Journal of Chromatography A 2018-09, Vol.1568, p.123-130
Hauptverfasser:	Zhao, Yu-Mei, Wang, Lv-Huan, Luo, Si-Fan, Wang, Qi-Qin, Moaddel, Ruin, Zhang, Ting-Ting, Jiang, Zheng-Jin
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Products - chemistry Biological Products - pharmacology catalytic activity chromatography Chromatography, High Pressure Liquid Drug Discovery Drug Evaluation, Preclinical drugs enzyme activity Enzyme immobilization enzyme inhibition Enzyme Inhibitors - analysis Enzyme Inhibitors - chemistry Enzyme Inhibitors - pharmacology glycosides hydrolysis Image Processing, Computer-Assisted immobilized enzymes influenza Kinetics Ligands luteolin lycorine Magnetic beads Magnetics magnetism Microspheres Neuraminidase Neuraminidase - metabolism oseltamivir plant extracts Plant Extracts - analysis Reproducibility of Results screening Screening inhibitors secondary metabolites sialidase Small Molecule Libraries Tandem Mass Spectrometry therapeutics
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