The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel
Background and Purpose 9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we te...
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Veröffentlicht in: | British journal of pharmacology 2018-12, Vol.175 (23), p.4325-4337 |
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creator | Hou, Jian‐wen Fei, Yu‐dong Li, Wei Chen, Yi‐he Wang, Qian Xiao, Ying Wang, Yue‐peng Li, Yi‐gang |
description | Background and Purpose
9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9‐phenanthrol.
Experimental Approach
Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild‐type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX‐II) was used to amplify the late sodium current (INaL) and induce arrhythmias. Whole‐cell patch clamp technique was used to record APs and ionic currents.
Key Results
9‐Phenanthrol (10–50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX‐II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9‐phenanthrol modulated the gating properties of cardiac sodium channels and dose‐dependently inhibited INaL and peak sodium current (INaP) in VMs with an IC50 of 18 and 71.5 μM respectively. Its ability to inhibit INaL was further confirmed in PCs and HEK293 cells expressing SCN5A mutations.
Conclusions and Implications
Our results indicate that 9‐phenanthrol modulates the gating properties of cardiac sodium channels and inhibits INaL and INaP, which may contribute to its antiarrhythmic and cardioprotective effects. |
doi_str_mv | 10.1111/bph.14490 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6240128</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2134093277</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4430-56e379d1b4e216c38fbde9fc6860243a527cc6cfc194ff681c674505c13b258d3</originalsourceid><addsrcrecordid>eNp1kctKxDAUhoMoOo4ufAEJuHLRMbem7UbQwRsIutB1SNPURjpJTVLFnY_gM_okRkdFF2YTwvnynXP4AdjBaIbTOaiHboYZq9AKmGBW8CynJV4FE4RQkWFclhtgM4R7hFKxyNfBBkU4p5SwCRhuOg2jlzYYbSP0WukhOg8HF9PbyB4udC9DlNFYyKDqpLW6h8Z2pjYfYPX28jp02kobO-8S7pqxl1EHqKRvjFQwuMaMi--vW2CtlX3Q21_3FNyentzMz7PLq7OL-dFlphijKMu5pkXV4JppgrmiZVs3umoVLzkijMqcFEpx1SpcsbblJVa8YDnKFaY1ycuGTsHh0juM9UI3Km3jZS8GbxbSPwsnjfhbsaYTd-5RcMIQJmUS7H0JvHsYdYji3o3eppkFwZShipKiSNT-klLeheB1-9MBI_ERjkjhiM9wErv7e6Qf8juNBBwsgSfT6-f_TeL4-nypfAds8pxo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2134093277</pqid></control><display><type>article</type><title>The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel</title><source>MEDLINE</source><source>Wiley Online Library Free Content</source><source>Access via Wiley Online Library</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Hou, Jian‐wen ; Fei, Yu‐dong ; Li, Wei ; Chen, Yi‐he ; Wang, Qian ; Xiao, Ying ; Wang, Yue‐peng ; Li, Yi‐gang</creator><creatorcontrib>Hou, Jian‐wen ; Fei, Yu‐dong ; Li, Wei ; Chen, Yi‐he ; Wang, Qian ; Xiao, Ying ; Wang, Yue‐peng ; Li, Yi‐gang</creatorcontrib><description>Background and Purpose
9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9‐phenanthrol.
Experimental Approach
Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild‐type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX‐II) was used to amplify the late sodium current (INaL) and induce arrhythmias. Whole‐cell patch clamp technique was used to record APs and ionic currents.
Key Results
9‐Phenanthrol (10–50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX‐II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9‐phenanthrol modulated the gating properties of cardiac sodium channels and dose‐dependently inhibited INaL and peak sodium current (INaP) in VMs with an IC50 of 18 and 71.5 μM respectively. Its ability to inhibit INaL was further confirmed in PCs and HEK293 cells expressing SCN5A mutations.
Conclusions and Implications
Our results indicate that 9‐phenanthrol modulates the gating properties of cardiac sodium channels and inhibits INaL and INaP, which may contribute to its antiarrhythmic and cardioprotective effects.</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1111/bph.14490</identifier><identifier>PMID: 30153324</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animals ; Anti-Arrhythmia Agents - pharmacology ; Antiarrhythmics ; Cardiac arrhythmia ; Cardiac muscle ; Channel gating ; Dose-Response Relationship, Drug ; EKG ; Female ; Heart ; HEK293 Cells ; Humans ; Male ; Myocytes ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Phenanthrenes - pharmacology ; Purkinje cells ; Rabbits ; Research Paper ; Research Papers ; Sodium ; Sodium channels ; Transient receptor potential proteins ; TRPM Cation Channels - antagonists & inhibitors ; TRPM Cation Channels - metabolism ; Ventricle ; Wedges</subject><ispartof>British journal of pharmacology, 2018-12, Vol.175 (23), p.4325-4337</ispartof><rights>2018 The British Pharmacological Society</rights><rights>2018 The British Pharmacological Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4430-56e379d1b4e216c38fbde9fc6860243a527cc6cfc194ff681c674505c13b258d3</citedby><cites>FETCH-LOGICAL-c4430-56e379d1b4e216c38fbde9fc6860243a527cc6cfc194ff681c674505c13b258d3</cites><orcidid>0000-0003-3007-7212</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240128/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240128/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30153324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hou, Jian‐wen</creatorcontrib><creatorcontrib>Fei, Yu‐dong</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Chen, Yi‐he</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Xiao, Ying</creatorcontrib><creatorcontrib>Wang, Yue‐peng</creatorcontrib><creatorcontrib>Li, Yi‐gang</creatorcontrib><title>The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>Background and Purpose
9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9‐phenanthrol.
Experimental Approach
Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild‐type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX‐II) was used to amplify the late sodium current (INaL) and induce arrhythmias. Whole‐cell patch clamp technique was used to record APs and ionic currents.
Key Results
9‐Phenanthrol (10–50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX‐II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9‐phenanthrol modulated the gating properties of cardiac sodium channels and dose‐dependently inhibited INaL and peak sodium current (INaP) in VMs with an IC50 of 18 and 71.5 μM respectively. Its ability to inhibit INaL was further confirmed in PCs and HEK293 cells expressing SCN5A mutations.
Conclusions and Implications
Our results indicate that 9‐phenanthrol modulates the gating properties of cardiac sodium channels and inhibits INaL and INaP, which may contribute to its antiarrhythmic and cardioprotective effects.</description><subject>Animals</subject><subject>Anti-Arrhythmia Agents - pharmacology</subject><subject>Antiarrhythmics</subject><subject>Cardiac arrhythmia</subject><subject>Cardiac muscle</subject><subject>Channel gating</subject><subject>Dose-Response Relationship, Drug</subject><subject>EKG</subject><subject>Female</subject><subject>Heart</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Male</subject><subject>Myocytes</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Phenanthrenes - pharmacology</subject><subject>Purkinje cells</subject><subject>Rabbits</subject><subject>Research Paper</subject><subject>Research Papers</subject><subject>Sodium</subject><subject>Sodium channels</subject><subject>Transient receptor potential proteins</subject><subject>TRPM Cation Channels - antagonists & inhibitors</subject><subject>TRPM Cation Channels - metabolism</subject><subject>Ventricle</subject><subject>Wedges</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctKxDAUhoMoOo4ufAEJuHLRMbem7UbQwRsIutB1SNPURjpJTVLFnY_gM_okRkdFF2YTwvnynXP4AdjBaIbTOaiHboYZq9AKmGBW8CynJV4FE4RQkWFclhtgM4R7hFKxyNfBBkU4p5SwCRhuOg2jlzYYbSP0WukhOg8HF9PbyB4udC9DlNFYyKDqpLW6h8Z2pjYfYPX28jp02kobO-8S7pqxl1EHqKRvjFQwuMaMi--vW2CtlX3Q21_3FNyentzMz7PLq7OL-dFlphijKMu5pkXV4JppgrmiZVs3umoVLzkijMqcFEpx1SpcsbblJVa8YDnKFaY1ycuGTsHh0juM9UI3Km3jZS8GbxbSPwsnjfhbsaYTd-5RcMIQJmUS7H0JvHsYdYji3o3eppkFwZShipKiSNT-klLeheB1-9MBI_ERjkjhiM9wErv7e6Qf8juNBBwsgSfT6-f_TeL4-nypfAds8pxo</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Hou, Jian‐wen</creator><creator>Fei, Yu‐dong</creator><creator>Li, Wei</creator><creator>Chen, Yi‐he</creator><creator>Wang, Qian</creator><creator>Xiao, Ying</creator><creator>Wang, Yue‐peng</creator><creator>Li, Yi‐gang</creator><general>Blackwell Publishing Ltd</general><general>John Wiley and Sons Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3007-7212</orcidid></search><sort><creationdate>201812</creationdate><title>The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel</title><author>Hou, Jian‐wen ; Fei, Yu‐dong ; Li, Wei ; Chen, Yi‐he ; Wang, Qian ; Xiao, Ying ; Wang, Yue‐peng ; Li, Yi‐gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4430-56e379d1b4e216c38fbde9fc6860243a527cc6cfc194ff681c674505c13b258d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Anti-Arrhythmia Agents - pharmacology</topic><topic>Antiarrhythmics</topic><topic>Cardiac arrhythmia</topic><topic>Cardiac muscle</topic><topic>Channel gating</topic><topic>Dose-Response Relationship, Drug</topic><topic>EKG</topic><topic>Female</topic><topic>Heart</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Male</topic><topic>Myocytes</topic><topic>Myocytes, Cardiac - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Phenanthrenes - pharmacology</topic><topic>Purkinje cells</topic><topic>Rabbits</topic><topic>Research Paper</topic><topic>Research Papers</topic><topic>Sodium</topic><topic>Sodium channels</topic><topic>Transient receptor potential proteins</topic><topic>TRPM Cation Channels - antagonists & inhibitors</topic><topic>TRPM Cation Channels - metabolism</topic><topic>Ventricle</topic><topic>Wedges</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Jian‐wen</creatorcontrib><creatorcontrib>Fei, Yu‐dong</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Chen, Yi‐he</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Xiao, Ying</creatorcontrib><creatorcontrib>Wang, Yue‐peng</creatorcontrib><creatorcontrib>Li, Yi‐gang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Jian‐wen</au><au>Fei, Yu‐dong</au><au>Li, Wei</au><au>Chen, Yi‐he</au><au>Wang, Qian</au><au>Xiao, Ying</au><au>Wang, Yue‐peng</au><au>Li, Yi‐gang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>2018-12</date><risdate>2018</risdate><volume>175</volume><issue>23</issue><spage>4325</spage><epage>4337</epage><pages>4325-4337</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><abstract>Background and Purpose
9‐Phenanthrol, known as a specific inhibitor of the transient receptor potential melastatin 4 (TRMP4) channel, has been shown to modulate cardiac electrical activity and exert antiarrhythmic effects. However, its pharmacological effects remain to be fully explored. Here, we tested the hypothesis that cardiac sodium current inhibition contributes to the cardioprotective effect of 9‐phenanthrol.
Experimental Approach
Single ventricular myocytes (VMs) and Purkinje cells (PCs) were enzymatically isolated from rabbits. Arterially perfused rabbit wedge preparations were also used, and transmural electrocardiogram and endocardial action potentials (APs) were simultaneously recorded. Wild‐type and mutated human recombinant SCN5A were expressed in HEK293 cells. Anemonia toxin II (ATX‐II) was used to amplify the late sodium current (INaL) and induce arrhythmias. Whole‐cell patch clamp technique was used to record APs and ionic currents.
Key Results
9‐Phenanthrol (10–50 μM) stabilized ventricular repolarization and abolished arrhythmias induced by ATX‐II in both isolated VMs, PCs and wedge preparations. Further study revealed that 9‐phenanthrol modulated the gating properties of cardiac sodium channels and dose‐dependently inhibited INaL and peak sodium current (INaP) in VMs with an IC50 of 18 and 71.5 μM respectively. Its ability to inhibit INaL was further confirmed in PCs and HEK293 cells expressing SCN5A mutations.
Conclusions and Implications
Our results indicate that 9‐phenanthrol modulates the gating properties of cardiac sodium channels and inhibits INaL and INaP, which may contribute to its antiarrhythmic and cardioprotective effects.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>30153324</pmid><doi>10.1111/bph.14490</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3007-7212</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Anti-Arrhythmia Agents - pharmacology Antiarrhythmics Cardiac arrhythmia Cardiac muscle Channel gating Dose-Response Relationship, Drug EKG Female Heart HEK293 Cells Humans Male Myocytes Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism Phenanthrenes - pharmacology Purkinje cells Rabbits Research Paper Research Papers Sodium Sodium channels Transient receptor potential proteins TRPM Cation Channels - antagonists & inhibitors TRPM Cation Channels - metabolism Ventricle Wedges |
title | The transient receptor potential melastatin 4 channel inhibitor 9‐phenanthrol modulates cardiac sodium channel |
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