A monovalent ion in the DNA binding interface of the eukaryotic junction-resolving enzyme GEN1

Abstract GEN1 is a member of the FEN/EXO family of structure-selective nucleases that cleave 1 nt 3′ to a variety of branchpoints. For each, the H2TH motif binds a monovalent ion and plays an important role in binding one helical arm of the substrates. We investigate here the importance of this meta...

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Veröffentlicht in:	Nucleic acids research 2018-11, Vol.46 (20), p.11089-11098
Hauptverfasser:	Liu, Yijin, Freeman, Alasdair DJ, Déclais, Anne-Cécile, Lilley, David M J
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalytic Domain - genetics Chaetomium - enzymology Chaetomium - genetics Chaetomium - metabolism Cloning, Molecular Crystallography, X-Ray DNA Cleavage DNA, Cruciform - metabolism DNA, Fungal - metabolism Escherichia coli Holliday Junction Resolvases - chemistry Holliday Junction Resolvases - genetics Holliday Junction Resolvases - metabolism Ions - chemistry Protein Binding Protein Interaction Domains and Motifs - genetics Structural Biology Substrate Specificity - genetics
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