Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Proteins are an important class of biological macromolecules that play many key roles in cellular functions including gene expression, catalyzing metabolic reactions, DNA repair and replication. Therefore, a detailed understanding of these processes provides critical information on how cells functio...

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Veröffentlicht in:Journal of visualized experiments 2018-10 (140)
Hauptverfasser: Ahdash, Zainab, Lau, Andy M, Martens, Chloe, Politis, Argyris
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container_title Journal of visualized experiments
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creator Ahdash, Zainab
Lau, Andy M
Martens, Chloe
Politis, Argyris
description Proteins are an important class of biological macromolecules that play many key roles in cellular functions including gene expression, catalyzing metabolic reactions, DNA repair and replication. Therefore, a detailed understanding of these processes provides critical information on how cells function. Integrative structural MS methods offer structural and dynamical information on protein complex assembly, complex connectivity, subunit stoichiometry, protein oligomerization and ligand binding. Recent advances in integrative structural MS have allowed for the characterization of challenging biological systems including large DNA binding proteins and membrane proteins. This protocol describes how to integrate diverse MS data such as native MS and ion mobility-mass spectrometry (IM-MS) with molecular dynamics simulations to gain insights into a helicase-nuclease DNA repair protein complex. The resulting approach provides a framework for detailed studies of ligand binding to other protein complexes involved in important biological processes.
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subjects Chemistry
Ion Mobility Spectrometry
Ligands
Mass Spectrometry
Molecular Dynamics Simulation
Multiprotein Complexes - chemistry
Multiprotein Complexes - metabolism
Protein Binding
Protein Conformation
Proteins - chemistry
Proteins - metabolism
title Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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