Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract
Siwei Jianghuang Decoction Powder (SWJH) documented originally in the Four Medical Tantras-Blue Glaze exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyll...
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| creator | Lai, Xianrong Tong, Dong Ai, Xiaopeng Wu, Jiasi Luo, Yu Zuo, Fang Wei, Zhicheng Li, Yanqiao Huang, Wanyi Wang, Wenqian Jiang, Qing Meng, Xianli Zeng, Yong Wang, Ping |
| description | Siwei Jianghuang Decoction Powder (SWJH) documented originally in
the Four Medical Tantras-Blue Glaze
exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression. |
| doi_str_mv | 10.1038/s41598-018-35148-2 |
| format | Article |
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the Four Medical Tantras-Blue Glaze
exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-35148-2</identifier><identifier>PMID: 30420600</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/51 ; 38/1 ; 38/77 ; 64/60 ; 692/163/2743/137/138 ; 692/4022/1585/2759/1419 ; 82/1 ; 82/51 ; 82/80 ; 96/63 ; Blood ; Body weight ; Creatinine ; Diabetes ; Diabetes mellitus ; Diabetic nephropathy ; Enzyme-linked immunosorbent assay ; Excretion ; Fibrosis ; Food intake ; Gene expression ; Glucose ; Humanities and Social Sciences ; Hypoxia ; Kidneys ; mRNA ; multidisciplinary ; Nephropathy ; Powder ; Principal components analysis ; Rodents ; Science ; Science (multidisciplinary) ; Transforming growth factor ; Transforming growth factor-b1 ; Urea ; Uric acid ; Urine ; Vascular endothelial growth factor</subject><ispartof>Scientific reports, 2018-11, Vol.8 (1), p.16707-11, Article 16707</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-ed884055864f29d6f0a00c0c912cf21dd130eb7ca1605ae8d29a8bcdd86ef8323</citedby><cites>FETCH-LOGICAL-c474t-ed884055864f29d6f0a00c0c912cf21dd130eb7ca1605ae8d29a8bcdd86ef8323</cites><orcidid>0000-0002-1133-1505</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232159/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6232159/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30420600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lai, Xianrong</creatorcontrib><creatorcontrib>Tong, Dong</creatorcontrib><creatorcontrib>Ai, Xiaopeng</creatorcontrib><creatorcontrib>Wu, Jiasi</creatorcontrib><creatorcontrib>Luo, Yu</creatorcontrib><creatorcontrib>Zuo, Fang</creatorcontrib><creatorcontrib>Wei, Zhicheng</creatorcontrib><creatorcontrib>Li, Yanqiao</creatorcontrib><creatorcontrib>Huang, Wanyi</creatorcontrib><creatorcontrib>Wang, Wenqian</creatorcontrib><creatorcontrib>Jiang, Qing</creatorcontrib><creatorcontrib>Meng, Xianli</creatorcontrib><creatorcontrib>Zeng, Yong</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><title>Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Siwei Jianghuang Decoction Powder (SWJH) documented originally in
the Four Medical Tantras-Blue Glaze
exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression.</description><subject>13/1</subject><subject>13/51</subject><subject>38/1</subject><subject>38/77</subject><subject>64/60</subject><subject>692/163/2743/137/138</subject><subject>692/4022/1585/2759/1419</subject><subject>82/1</subject><subject>82/51</subject><subject>82/80</subject><subject>96/63</subject><subject>Blood</subject><subject>Body weight</subject><subject>Creatinine</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetic nephropathy</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Excretion</subject><subject>Fibrosis</subject><subject>Food intake</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Humanities and Social Sciences</subject><subject>Hypoxia</subject><subject>Kidneys</subject><subject>mRNA</subject><subject>multidisciplinary</subject><subject>Nephropathy</subject><subject>Powder</subject><subject>Principal components analysis</subject><subject>Rodents</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transforming growth factor</subject><subject>Transforming growth factor-b1</subject><subject>Urea</subject><subject>Uric acid</subject><subject>Urine</subject><subject>Vascular endothelial growth 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Decoction Powder extract</title><author>Lai, Xianrong ; Tong, Dong ; Ai, Xiaopeng ; Wu, Jiasi ; Luo, Yu ; Zuo, Fang ; Wei, Zhicheng ; Li, Yanqiao ; Huang, Wanyi ; Wang, Wenqian ; Jiang, Qing ; Meng, Xianli ; Zeng, Yong ; Wang, Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-ed884055864f29d6f0a00c0c912cf21dd130eb7ca1605ae8d29a8bcdd86ef8323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>13/1</topic><topic>13/51</topic><topic>38/1</topic><topic>38/77</topic><topic>64/60</topic><topic>692/163/2743/137/138</topic><topic>692/4022/1585/2759/1419</topic><topic>82/1</topic><topic>82/51</topic><topic>82/80</topic><topic>96/63</topic><topic>Blood</topic><topic>Body weight</topic><topic>Creatinine</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetic nephropathy</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Excretion</topic><topic>Fibrosis</topic><topic>Food intake</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Humanities and Social Sciences</topic><topic>Hypoxia</topic><topic>Kidneys</topic><topic>mRNA</topic><topic>multidisciplinary</topic><topic>Nephropathy</topic><topic>Powder</topic><topic>Principal components analysis</topic><topic>Rodents</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Transforming growth factor</topic><topic>Transforming growth factor-b1</topic><topic>Urea</topic><topic>Uric acid</topic><topic>Urine</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lai, Xianrong</creatorcontrib><creatorcontrib>Tong, Dong</creatorcontrib><creatorcontrib>Ai, Xiaopeng</creatorcontrib><creatorcontrib>Wu, Jiasi</creatorcontrib><creatorcontrib>Luo, Yu</creatorcontrib><creatorcontrib>Zuo, 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Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-11-12</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>16707</spage><epage>11</epage><pages>16707-11</pages><artnum>16707</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Siwei Jianghuang Decoction Powder (SWJH) documented originally in
the Four Medical Tantras-Blue Glaze
exhibited beneficial effects on diabetic nephropathy (DN) via combined synergistically action of multiple formula components including Curcumae longae Rhizoma, Berberidis dictyophyllae Cortex, Phyllanthi Fructus and Tribuli Fructus. This study investigated the effects of SWJH on DN in db/db mice and possible underlying mechanisms. The ten weeks old db/db mice treated with SWJH by intra-gastric administration once a day for 8 weeks. After 8 weeks, body weight, water and food intake of mice were recorded. The level of fasting blood glucose (FBG) was measured. Serum creatinine (Scr), blood urea nitrogen (BUN), urine microalbumin (UMAlb), serum uric acid (UA) and urinary albumin excretion (UAE) were detected. An enzyme-linked immunosorbent assay was performed to test serum vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). Real-time PCR and Western blot analysis were used to test mRNA and protein expression of hypoxia inducible factor-1α (HIF-1α), VEGF and TGF-β1 in kidney tissue. SWJH treatment significantly reduced the levels of FBG, Scr, BUN, UMAlb, UA and UAE and retarded renal fibrosis. SWJH treatment further significantly reduced serum TGF-β1 level and downregulated the expression of HIF-1α, VEGF and TGF-β1 at both mRNA and protein levels. Principal component analysis and partial least squares regression and hierarchical cluster analysis demonstrated that SWJH treatment significantly ameliorated renal damage in DN mice. These consequences suggested that SWJH formulations were effective in the treatment of DN through regulating the HIF-1α, VEGF and TGF-β1 overexpression.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30420600</pmid><doi>10.1038/s41598-018-35148-2</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-1133-1505</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/51 38/1 38/77 64/60 692/163/2743/137/138 692/4022/1585/2759/1419 82/1 82/51 82/80 96/63 Blood Body weight Creatinine Diabetes Diabetes mellitus Diabetic nephropathy Enzyme-linked immunosorbent assay Excretion Fibrosis Food intake Gene expression Glucose Humanities and Social Sciences Hypoxia Kidneys mRNA multidisciplinary Nephropathy Powder Principal components analysis Rodents Science Science (multidisciplinary) Transforming growth factor Transforming growth factor-b1 Urea Uric acid Urine Vascular endothelial growth factor |
| title | Amelioration of diabetic nephropathy in db/db mice treated with tibetan medicine formula Siwei Jianghuang Decoction Powder extract |
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