Tripeptide binding in a proton‐dependent oligopeptide transporter
Proton‐dependent oligopeptide transporters (POTs) are important for the uptake of di‐/tripeptides in many organisms and for drug transport in humans. The binding mode of dipeptides has been well described. However, it is still debated how tripeptides are recognized. Here, we show that tripeptides of...
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| Veröffentlicht in: | FEBS letters 2018-10, Vol.592 (19), p.3239-3247 |
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| creator | Martinez Molledo, Maria Quistgaard, Esben M. Löw, Christian |
| description | Proton‐dependent oligopeptide transporters (POTs) are important for the uptake of di‐/tripeptides in many organisms and for drug transport in humans. The binding mode of dipeptides has been well described. However, it is still debated how tripeptides are recognized. Here, we show that tripeptides of the sequence Phe‐Ala‐Xxx bind with similar affinities as dipeptides to the POT transporter from Streptococcus thermophilus (PepTSt). We furthermore determined a 2.3‐Å structure of PepTSt in complex with Phe‐Ala‐Gln. The phenylalanine and alanine residues of the peptide adopt the same positions as previously observed for the Phe‐Ala dipeptide, while the glutamine side chain extends into a hitherto uncharacterized pocket. This pocket is adaptable in size and can likely accommodate a wide variety of peptide side chains. |
| doi_str_mv | 10.1002/1873-3468.13246 |
| format | Article |
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The binding mode of dipeptides has been well described. However, it is still debated how tripeptides are recognized. Here, we show that tripeptides of the sequence Phe‐Ala‐Xxx bind with similar affinities as dipeptides to the POT transporter from Streptococcus thermophilus (PepTSt). We furthermore determined a 2.3‐Å structure of PepTSt in complex with Phe‐Ala‐Gln. The phenylalanine and alanine residues of the peptide adopt the same positions as previously observed for the Phe‐Ala dipeptide, while the glutamine side chain extends into a hitherto uncharacterized pocket. This pocket is adaptable in size and can likely accommodate a wide variety of peptide side chains.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1002/1873-3468.13246</identifier><identifier>PMID: 30194725</identifier><language>eng</language><publisher>England: John Wiley and Sons Inc</publisher><subject>Communication ; major facilitator superfamily (MFS) ; membrane protein crystallography ; peptide binding ; peptide transporters ; POTs</subject><ispartof>FEBS letters, 2018-10, Vol.592 (19), p.3239-3247</ispartof><rights>2018 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.</rights><rights>2018 The Authors. 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This pocket is adaptable in size and can likely accommodate a wide variety of peptide side chains.</description><subject>Communication</subject><subject>major facilitator superfamily (MFS)</subject><subject>membrane protein crystallography</subject><subject>peptide binding</subject><subject>peptide transporters</subject><subject>POTs</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqFkLtOwzAUQC0EoqUws6GMLGn9ipMsSFC1gITEUmbLjW-KUWoHOwV14xP4Rr6ElLQVTEyW7ePjq4PQOcFDgjEdkSxlMeMiGxJGuThA_f3JIepjTHicpDnroZMQXnC7z0h-jHoMk5ynNOmj8cybGurGaIjmxmpjF5GxkYpq7xpnvz4-NdRgNdgmcpVZuB3ceGVD7XwD_hQdlaoKcLZdB-hpOpmN7-KHx9v78fVDXCRYiFgknBUlQEY1p1wrXGKq05IITaBkiU4ZFrxMOGCaFxlJFWMFJFByTQrIOWYDdNV569V8CbpoZ_KqkrU3S-XX0ikj_95Y8ywX7k0KSglORCu43Aq8e11BaOTShAKqSllwqyBbilCRU0FadNShhXcheCj33xAsN-nlJrTchJY_6dsXF7-n2_O71i0gOuDdVLD-zyenkxvamb8B8OCQig</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Martinez Molledo, Maria</creator><creator>Quistgaard, Esben M.</creator><creator>Löw, Christian</creator><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201810</creationdate><title>Tripeptide binding in a proton‐dependent oligopeptide transporter</title><author>Martinez Molledo, Maria ; Quistgaard, Esben M. ; Löw, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5066-6543cfee82d424da0f02d7f16d1ef35d73064f54e029c817a33ce5ef4d1ce9403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Communication</topic><topic>major facilitator superfamily (MFS)</topic><topic>membrane protein crystallography</topic><topic>peptide binding</topic><topic>peptide transporters</topic><topic>POTs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez Molledo, Maria</creatorcontrib><creatorcontrib>Quistgaard, Esben M.</creatorcontrib><creatorcontrib>Löw, Christian</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez Molledo, Maria</au><au>Quistgaard, Esben M.</au><au>Löw, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tripeptide binding in a proton‐dependent oligopeptide transporter</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2018-10</date><risdate>2018</risdate><volume>592</volume><issue>19</issue><spage>3239</spage><epage>3247</epage><pages>3239-3247</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Proton‐dependent oligopeptide transporters (POTs) are important for the uptake of di‐/tripeptides in many organisms and for drug transport in humans. 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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2018-10, Vol.592 (19), p.3239-3247 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6221056 |
| source | Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Communication major facilitator superfamily (MFS) membrane protein crystallography peptide binding peptide transporters POTs |
| title | Tripeptide binding in a proton‐dependent oligopeptide transporter |
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