TMIC-30. COMPUTATIONAL CHARACTERIZATION OF SUPPRESSIVE IMMUNE MICROENVIRONMENTS IN GLIOBLASTOMA

Abstract The immunosuppressive microenvironment in glioblastoma (GBM) prevents efficient antitumoral immune response and thus enables tumor formation and growth. An understanding of the nature of immunosuppression is still largely lacking although it is important for successful cancer treatment through immune system modulation. To gain insight into immunosuppression in GBM, we performed a computational analysis to model the relative immune cell content and type of immune response in each GBM tumor sample from the Cancer Genome Atlas RNA-seq dataset. As a result, we uncovered high variability in immune system-related responses and in the composition of microenvironment across the cohort, suggesting immunological diversity. Immune cell compositions were associated with typical alterations, such as IDH mutation or inactivating NF1 mutation/deletion. Furthermore, our analysis identified three GBM subgroups presenting different adaptive immune responses: Negative, Humoral, and Cellular-like. These subgroups were linked to transcriptional GBM subtypes and typical genetic alterations. Interestingly, all the G-CIMP and IDH mutated samples were in the Negative group, which was also enriched by cases with focal amplification of CDK4 and MARCH9. Overall, our analysis revealed heterogeneity in the immune microenvironment of GBM and identified MARCH9 amplification as a possible new marker for immunosuppression. Characterization of diverse immune responses will facilitate patient stratification improving personalized immunotherapy in the future.